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Genetic relationships and genotype tracing in date palms (Phoenix dactylifera L.) in Oman, based on microsatellite markers

Published online by Cambridge University Press:  01 April 2008

Ishaq Ahmed Al-Ruqaishi
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
Michael Davey
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
Peter Alderson
Affiliation:
Faculty of Health and Biological Sciences, University of Nottingham, Malaysia Campus, Jalan Broga 43500, Semenyih, Malaysia
Sean Mayes*
Affiliation:
School of Biosciences, University of Nottingham, Sutton Bonington Campus, Loughborough LE12 5RD, UK
*
*Corresponding author. E-mail: sean.mayes@nottingham.ac.uk

Abstract

Microsatellite markers (SSRs) were used to screen and analyse the genetic diversity among clonal genotypes of date palm (Phoenix dactylifera L.) derived by somatic embryogenesis in Oman. Twenty-one palms, representing 14 Omani, five Bahraini, one Iraqi and one Moroccan genotype, were screened with ten microsatellite markers. All primer pairs produced an amplification product in the expected size range and detected high levels of polymorphism among the analysed samples. Correspondence analysis revealed that the genotypes from Bahrain and Iraq showed a close relationship with accessions already grown in Oman. The genotype from Morocco (Medjool) appeared distinct from the rest of the material. Three independent clonal lines derived from a single Khalas Aldahra genotype were found to give identical genetic fingerprints. The value of this work for date palm production and conservation in Oman is discussed.

Type
Short Communication
Copyright
Copyright © NIAB 2008

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References

Anonymous(2002) Statistical Year Book. Issue 12. Muscat, Oman: Directorate General of National Statistics, Ministry of Agriculture and Fisheries.Google Scholar
Ben Abdallah, A, Stiti, K and Lepoivre, P (2000) Identification de cultivars de palmier dattier (Phoenix dactylifera L.) par l'amplification ale'atoire d'ADN (RAPD). Cahiers d'Agriculture 9: 103107.Google Scholar
Billotte, N, Marseillac, N, Brottier, P, Noyer, JL, Jacquemoud-Collet, JP, Moreau, C, Couvreur, T, Chevallier, MH, Pintaud, JC and Risterucci, AM (2004) Nuclear microsatellite marker for date palm (Phoenix dactylifera): characterization and utility across the genus Phoenix and in other palm genera. Molecular Ecology Notes 4: 256258.CrossRefGoogle Scholar
Goodman, MM (1972) Distance analysis in biology. Systematic Zoology 21: 174186.CrossRefGoogle Scholar
Sedra, MH, Lashermes, P, Trouslot, P, Combes, MC and Hamon, S (1998) Identification and genetic diversity analysis of date palm (Phoenix dactylifera L.) varieties from Morocco using RAPD markers. Euphytica 103: 7582.CrossRefGoogle Scholar
Trifi, M, Rhouma, A and Marrakchi, M (2000) Phylogenetic relationships in Tunisian date palm (Phoenix dactylifera L.) germplasm collection using DNA amplification fingerprinting. Agronomie 20: 665671.CrossRefGoogle Scholar
Zaid, A and Arias-Jiménez, EJ (2002) Date Palm Cultivation. FAO Plant Production and Protection No. 156, Rev. 1. Rome: FAO, p. 110.Google Scholar
Zehdi, S, Sakka, H, Rhouma, A, Ould, MSA, Marrakchi, M and Trifi, M (2004a) Analysis of Tunisian date palm germplasm using simple sequence repeat primers. African Journal of Biotechnology 3: 215219.CrossRefGoogle Scholar
Zehdi, S, Trifi, M, Billotte, N, Marrackhi, M and Pintaud, JC (2004b) Genetic diversity of Tunisian date palms (Phoenix dactylifera L.) revealed by nuclear microsatellite polymorphism. Hereditas 141: 278287.CrossRefGoogle Scholar