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Genetic structure analysis and genetic fingerprinting of pomegranate cultivars (Punica granatum L.) by using SCoT molecular markers

Published online by Cambridge University Press:  10 July 2023

Elham Sadeghi Seresht ,
Hamid Reza Karimi Open the ORCID record for Hamid Reza Karimi [Opens in a new window] ,
Khalil Malekzadeh ,
Seyed Hossein Mirdehghan  and
Ali Akbar Mohamadi Mirik
Elham Sadeghi Seresht
Affiliation:
Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
Hamid Reza Karimi*
Affiliation:
Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
Khalil Malekzadeh
Affiliation:
Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
Seyed Hossein Mirdehghan
Affiliation:
Department of Horticultural Science, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
Ali Akbar Mohamadi Mirik
Affiliation:
Department of Genetics and Crop Production, Faculty of Agriculture, Vali-e-Asr University of Rafsanjan, Rafsanjan, Iran
*
Corresponding author: Hamid Reza Karimi; E-mail: hrkarimi2017@gmail.com
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Abstract

Genetic diversity and genetic relatedness among 50 genotypes from eight countries, including Iran, Afghanistan, Turkmenistan, Syria, Lebanon, India, Yemen, and the United States located in two continents of Asia and the America, were assessed using SCoT markers. A total of 213 bands were produced; 100% of them were polymorphic; the average polymorphism information content (PIC) was 0.39. The mean Nei's gene diversity and Shannon's index were 0.33 and 0.49, respectively. Analysis of molecular variance suggested significant genetic differences within pomegranate populations. 99% of variance occurs within the populations, whereas 1% of the variation was recorded among the populations of pomegranate. Cluster analysis using SCoT markers able to group genotypes based on their geographical origins. Based on cluster analysis, the genotypes studied were divided into two main groups. The first group included most Asian genotypes, while American genotypes along with some Asian genotypes were in the second group. In the first group, Iranian genotypes were grouped with genotypes from Afghanistan and India. In the second group, the genotypes belonging to the America were in the same group as most of the genotypes of Turkmenistan. According to the present study, SCoT markers can be used to evaluate genetic diversity, identification and DNA fingerprinting pomegranate genotypes of different origins. This information can be used in breeding programs and the management of pomegranate collections.

Keywords

Genetic diversitypopulation structurePunica granatum LSCoT
Type
Research Article
Information
Plant Genetic Resources , Volume 21 , Issue 1 , February 2023 , pp. 37 - 44
Copyright
Copyright © The Author(s), 2023. Published by Cambridge University Press on behalf of National Institute of Agricultural Botany.

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