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Genetic variability in trait-specific rice germplasm groups based on coefficient of parentage, SSR markers and fertility restoration

Published online by Cambridge University Press:  12 May 2014

Pawan Khera ,
Akhilesh Kumar Singh ,
Rahul Priyadarshi ,
Durga Khandekar ,
Rajani K Allu ,
Chitkale Hiremath ,
Raj Kumar ,
Rashmi Mohan ,
K. Ulaganathan  and
Vinay Shenoy
Pawan Khera
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India International Crops Research Institute for the Semi-Arid Tropics (ICRISAT), Hyderabad, 502 324, India
Akhilesh Kumar Singh*
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Rahul Priyadarshi
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Durga Khandekar
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Rajani K Allu
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Chitkale Hiremath
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Raj Kumar
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
Rashmi Mohan
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
K. Ulaganathan
Affiliation:
Centre for Plant Molecular Biology, Osmania University, Hyderabad500 007, Andhra Pradesh, India
Vinay Shenoy
Affiliation:
Barwale Foundation, Barwale Chambers, # 3-6-666, Street No. 10, Himayathnagar, Hyderabad500 029, Andhra Pradesh, India
*
*Corresponding author. E-mail: akhileshsingh@barwalefoundation.org
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Abstract

To maximize heterosis, it is important to understand the genetic diversity of germplasm and associate useful phenotypic traits such as fertility restoration for hybrid rice breeding. The objectives of the present study were to characterize genetic diversity within a set of rice germplasm groups using coefficient of parentage (COP) values and 58 simple sequence repeat (SSR) markers for 124 genotypes having different attributes such as resistance/tolerance to various biotic and abiotic stresses. These lines were also used for identifying prospective restorers and maintainers for wild abortive-cytoplasmic male sterile (CMS) line. The mean COP value for all the lines was 0.11, indicating that the genotypes do not share common ancestry. The SSR analysis generated a total of 268 alleles with an average of 4.62 alleles per locus. The mean polymorphism information content value was 0.53, indicating that the markers selected were highly polymorphic. Grouping based on COP analysis revealed three major clusters pertaining to the indica, tropical japonica and japonica lines. A similar grouping pattern with some variation was also observed for the SSR markers. Fertility restoration phenotype based on the test cross of the 124 genotypes with a CMS line helped identify 23 maintainers, 58 restorers and 43 genotypes as either partial maintainers or partial restorers. This study demonstrates that COP analysis along with molecular marker analysis might encourage better organization of germplasm diversity and its use in hybrid rice breeding. Potential restorers identified in the study can be used for breeding high-yielding stress-tolerant medium-duration rice hybrids, while maintainers would prove useful for developing new rice CMS lines.

Keywords

coefficient of parentagecytoplasmic male sterilitygenetic diversityhybrid ricemaintainersmolecular markersrestorersspikelet fertility
Type
Research Article
Information
Plant Genetic Resources , Volume 13 , Issue 1 , April 2015 , pp. 56 - 67
Copyright
Copyright © NIAB 2014 

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