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Multiplex PCR effectively identifies tetraploid Triticum AABB – or AAGG-genome species

Published online by Cambridge University Press:  10 July 2017

Ken-Ichi Tanno ,
Ayaka Takeuchi ,
Eri Akahori ,
Keiko Kobayashi ,
Taihachi Kawahara  and
Kyoko Yamane
Ken-Ichi Tanno
Affiliation:
Graduate School of Sciences and Technology for Innovation, Yamaguchi University, Yoshida 1677-1, Yamaguchi City, Yamaguchi 753-8515, Japan
Ayaka Takeuchi
Affiliation:
Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu City, Gifu 501-1193, Japan
Eri Akahori
Affiliation:
Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu City, Gifu 501-1193, Japan
Keiko Kobayashi
Affiliation:
Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu City, Gifu 501-1193, Japan
Taihachi Kawahara
Affiliation:
Graduate School of Agriculture, Kyoto University, Nakajo, Mozume, Muko City, Kyoto 617-0001, Japan
Kyoko Yamane*
Affiliation:
Faculty of Applied Biological Sciences, Gifu University, 1-1, Yanagido, Gifu City, Gifu 501-1193, Japan
*
*Corresponding author. E-mail: kyamane@gifu-u.ac.jp
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Abstract

We developed a multiplex PCR DNA marker for quick and easy identification of the AAGG-genome timopheevii lineage, including Triticum timopheevii, Triticum araraticum and hexaploid Triticum zhukovskyi (AAAmAmGG), and the AABB-genome emmer wheat lineage, including Triticum durum, Triticum dicoccum and Triticum dicoccoides. Distinguishing between tetraploid AAGG- and AABB-genome wheat species based on morphology is known to be difficult. This multiplex PCR system is based on the simultaneous PCR amplification of two chloroplast regions, matK and rbcL. The matK region molecularly distinguishes the two lineages, whereas the rbcL region is a positive control amplicon. We also examined whether the simple sequence repeat is a fixed mutation within species, using genetic resources in the collection of KOMUGI, Kyoto University, which comprises accessioned species collected across diverse geographical areas. The multiplex PCR marker distinguished AAGG from AABB species with complete accuracy.

Keywords

emmergenotypingmatKrbcLtimopheeviiTriticum
Type
Short Communication
Information
Plant Genetic Resources , Volume 16 , Issue 3 , June 2018 , pp. 279 - 283
Copyright
Copyright © NIAB 2017 

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