Hostname: page-component-788cddb947-wgjn4 Total loading time: 0 Render date: 2024-10-18T16:06:10.752Z Has data issue: false hasContentIssue false
  • English
  • Français

Mass Propagation and Conservation of Podophyllum emodi Wall, an Endangered Medicinal Plant of the Himalaya

Published online by Cambridge University Press:  12 February 2007

Amit Chandra Kharkwal ,
Om Prakash ,
Amita Bhattacharya  and
Paramvir Singh Ahuja
Amit Chandra Kharkwal
Affiliation:
Institute of Himalayan Bioresource Technology Post Box No. 6, Palampur-176061, H.P, India
Om Prakash
Affiliation:
Institute of Himalayan Bioresource Technology Post Box No. 6, Palampur-176061, H.P, India
Amita Bhattacharya*
Affiliation:
Institute of Himalayan Bioresource Technology Post Box No. 6, Palampur-176061, H.P, India
Paramvir Singh Ahuja
Affiliation:
Institute of Himalayan Bioresource Technology Post Box No. 6, Palampur-176061, H.P, India
*
* Corresponding author. E-mail: amitabhatta@yahoo.co.uk
Get access Rights & Permissions [Opens in a new window]

Abstract

The technique of excised embryos was employed to facilitate the propagation of Podophyllum emodi derived from seeds collected at the different elevation zones of Himachal Pradesh, India. Seed germination was low, irrespective of stage of seed development or zone of collection. Germination was improved significantly (89.14%) in the partially mature and mature seeds collected from alpine and temperate zones when excised embryos were cultured on basal B5 medium. Leaf emergence and plant establishment in the field was also significantly higher in the plants raised through this technique, despite hypocotyl dormancy. The technique was successfully employed for the production of plants to be reintroduced in large numbers into their habitat in the Great Himalayan National Park.

Keywords

conservationexcised embryo culturemass propagationPodophyllum emodi
Type
Research Article
Information
Plant Genetic Resources , Volume 2 , Issue 1 , April 2004 , pp. 51 - 57
Copyright
© NIAB 2004

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

IHBT publication number 2215.

References

Airi, S, Rawal, RS, Dhar, U and Purohit, AN (1997) Population studies on P. hexandrum—a dwindling medicinal plant of the Himalaya. Plant Genetic Resources Newsletter 110: 29–34.Google Scholar
Arumugam, N and Bhojwani, SS (1990) Somatic embryogenesis in tissue cultures of Podophyllum hexandrum. Canadian Journal of Botany 68: 487–491.Google Scholar
Badhawar, RL and Sharma, BK (1963) A note on the germination of Podophyllum seeds. Indian Forestry 89: 445–447.Google Scholar
Bewley, JD and Black, M (1982) Mobilization of reserves. In: Physiology and Biochemistry of Seeds in Relation to Germination, Vol. 1. Heidelberg: Springer-Verlag, pp. 177–244.Google Scholar
Bhadula, SK, Singh, A, Lata, H, Kuniyal, CP and Purohit, AN (1996) Genetic resources of Podophyllum hexandrum Royle, an endangered medicinal species from Garhwal Himalaya. International Plant Genetic Resources Newsletter 106: 26–29.Google Scholar
Broomhead, AJ and Dewick, PM (1990) Tumor-inhibitory aryltetralin lignans in Himalayan mayapple, Podophyllum versipelle, Diphylleia cymosa, and Diphylleia grayi. Phytochemistry 29: 3831–3837.Google Scholar
Canel, C, Moraes, RM, Dayan, F and Ferreira, D (2000) Molecules of interest: podophyllotoxin. Phytochemistry 54: 115–120.Google Scholar
Collins, GB and Grosser, JW (1984) Culture of embryos. In: Vasil, IK (ed.) Cell Culture and Somatic Cell Genetics of Plants, Vol. 1. New York: Academic Press, pp. 241–257.Google Scholar
Dayan, FE, Canel, C, Kuhajek, J and Moraes, RM (2003) Purification and characterization of a β-glucosidase from Podophyllum peltatum catalyzing the conversion of podophyllotoxin-4-O-β-glucosidase to its podophyllotoxin aglycone. Biochimica et Biophysica Acta 1646(1-2): 157–163.Google Scholar
Foster, S (1989) Phytogeographic and botanical considerations of medicinal plants in Eastern Asia and Eastern North America. Herbs, Spices and Medicinal Plants 4: 115–144.Google Scholar
Fujii, Y (1991) Podophyllum spp. In vitro regeneration and the production of Podophyllotoxin. In: Bajaj, YPS (ed.) Biotechnology in Agriculture and Forestry, Vol. 15. Medicinal and Aromatic Plants III. Berlin: Springer-Verlag, pp. 362–375.Google Scholar
Gamborg, OL, Miller, RA and Ojima, V (1968) Nutrient requirements of suspension cultures of soybean root cells. Experimental Cell Research 50: 151–158.Google Scholar
Gomez, KA and Gomez, AA (1984) Statistical Procedures for Agricultural Research, 2nd edn. New York: John Wiley and Sons.Google Scholar
Jackson, DE and Dewick, PM (1984) Aryltetralin lignans from Podophyllum hexandrum and Podophyllum pleianthu. Phytochemistry 23: 1147–1152.Google Scholar
Jackson, DE and Dewick, PM (1985) Tumor inhibitory aryltetralin lignans from Podophyllum pleianthum. Phytochemistry 24: 2407–2409.Google Scholar
Kamil, WM and Dewick, PM (1986) Biosynthetic relationships of aryltetralin lignans to dibenzylbutyrolactone lignans. Phytochemistry 25: 2093–2102.Google Scholar
Korner, C (2001) Alpine ecosystems. In: Levin, SA (ed.) Encyclopedia of Biodiversity, Vol. 1. New York: Academic Press, pp. 133–144.Google Scholar
Lloyd, GB and McCown, BH (1980) Commercially feasible micro-propagation of mountain laurel (Kalmia latifolia) by use of shoot tip culture. Proceedings of the International Plant Propagation Society 30: 421–437.Google Scholar
Mallet, J (1996) The genetics of biological diversity: from varieties to species. In: Gatson, J (ed.) Biodiversity—A Biology of Number and Differences. Oxford: Blackwell Science, pp. 13–53.Google Scholar
McCown, BH and Sellmer, JC (1987) General media and vessels suitable for woody plant culture. In: Bonga, JM and Durjan, DJ (eds) Cell and Tissue Culture in Forestry, Vol. I. General Principle and Biotechnology. Amsterdam: Martinus Nijhoff Publishers, pp. 4–16.Google Scholar
Moraes, RM, Burandt, C, Ganzera, M, Li, X, Khan, I and Canel, C (2000) The American mayapple revisited—Podophyllum, peltatum—still a potential cash crop. Economic Botany 54: 471–476.Google Scholar
Moraes, RM, Bedir, E, Barrett, H, Burandt, C Jr, Canel, C and Khan, I (2001a) Evaluation of Podophyllum peltatum L. accessions for podophyllotoxin production. Planta Medica 68: 341–344.Google Scholar
Moraes, RM, Lata, H, Bedir, E, Maqbool, M and Cushman, K (2001b) The American mayapple and its potential for podophyllotoxin production. In: Janick, J (ed.) Trends in New Crops and New Uses. Alexandria, VA: ASHS Press, pp. 527–532.Google Scholar
Murashige, T and Skoog, F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Planta 15: 473–497.Google Scholar
Nadeem, M, Paini, LMS, Purohit, AN, Pandey, H and Nandi, SK (2000) Propagation and conservation of Podophyllum hexandrum Royle: an important medicinal herb. Biological Conservation 92: 121–129.Google Scholar
Parker, MA (1989) Disease impact and local genetic diversity in the clonal plant Podophyllum peltatum. Evolution 43: 540–547.Google Scholar
Purohit, AN and Nautiyal, MC (1986) Inhibitory effect of cotyledons on plumular development in two alpine rosettes. Canadian Journal of Botany 66: 205–206.Google Scholar
Rust, RW and Roth, RR (1981) Seed production and seedling establishment in the mayapple, Podophyllum peltatum L. American Midland Naturalist 105: 51–60.Google Scholar
Sadowska, A, Wiweger, M, Lata, B and Obidoska, G (1997) In vitro propagation of Podophyllum peltatum L. by the cultures of embrya and divided embrya. Biologia Plantarum 39: 331–336.Google Scholar
Silander, JA Jr (2001) Temperate forests. In: Levin, SA (ed.) Encyclopedia of Biodiversity, Vol. 5. New York: Academic Press, pp. 607–635.Google Scholar
Singh, A, Purohit, AN, Bhadula, SK, Lata, H, Kuniyal, CP and Chandra, S (1999) Seed production potential and germination behaviour in populations of Podophyllum hexandrum Royle. Journal of Plant Biology 26: 51–57.Google Scholar
Ziegler, P (1995) Carbohydrate degradation during germination. In: Kigel, J and Galili, G (eds) Seed Development and Germination. New York: Marcel Dekker, pp. 447–474.Google Scholar