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Proteomic identification of a fucosyltransferase from petals of milk thistle, Silybum marianum

Published online by Cambridge University Press:  16 July 2014

Siddhartha Kumar Mishra ,
Neelam S. Sangwan ,
Manoj Kumar Srivastava ,
Bhawana Mishra  and
Rajender Singh Sangwan
Siddhartha Kumar Mishra
Affiliation:
CSIR – Central Institute of Medicinal and Aromatic Plants, PO – CIMAP, Lucknow226 015, India
Neelam S. Sangwan
Affiliation:
CSIR – Central Institute of Medicinal and Aromatic Plants, PO – CIMAP, Lucknow226 015, India
Manoj Kumar Srivastava
Affiliation:
Department of Biochemistry, J.C. Bose Institute of Life Sciences, Bundelkhand University, Jhansi284 128, India
Bhawana Mishra
Affiliation:
CSIR – Central Institute of Medicinal and Aromatic Plants, PO – CIMAP, Lucknow226 015, India
Rajender Singh Sangwan*
Affiliation:
CSIR – Central Institute of Medicinal and Aromatic Plants, PO – CIMAP, Lucknow226 015, India
*
* Corresponding author. E-mail: sangwan.lab@gmail.com
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Abstract

Fucosyltransferases are a group of enzymes that catalyse the transfer of l-fucose from a donor substrate to an acceptor molecule. Silybum marianum is also called ‘milk thistle’ due to its characteristic flower shape. It produces two major flavonoids: silymarin and silybin. The plant and its major secondary metabolites are used for treatment/recovery after chronic liver disease, liver rehabilitation after hepatitis and treatment of gallbladder disease. These compounds also act as antioxidants for scavenging free radicals and inhibiting lipid peroxidation. We identified two peptide motifs (YYEAYLSHADEK and TTPDPSCGR designated as motif 1 and motif 2, respectively) of a fucosyltransferase derived from S. marianum that are highly conserved in its counterparts across the plant species and sources. The nature and properties of the motifs are discussed in terms of their putative participation in catalysis and enzyme/active site conformation.

Keywords

conserved motifsfucosylationfucosyltransferasesproteomicsSilybum marianum
Type
Research Article
Information
Plant Genetic Resources , Volume 12 , Issue S1: Special issue on the 3rd International Symposium on “Genomics of Plant Genetic Resources” , July 2014 , pp. S95 - S98
Copyright
Copyright © NIAB 2014 

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