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Characterization of microsatellite markers for Narcissus dubius, N. cuatrecasasii, N. assoanus and N. rupicola (Amaryllidaceae)

Published online by Cambridge University Press:  23 November 2018

Daniel Barranco Open the ORCID record for Daniel Barranco [Opens in a new window] ,
Violeta I. Simón-Porcar  and
Juan Arroyo
Daniel Barranco*
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
Violeta I. Simón-Porcar
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
Juan Arroyo
Affiliation:
Departamento de Biología Vegetal y Ecología, Universidad de Sevilla, Sevilla, Spain
*
*Corresponding author. E-mail: dnbarranco@gmail.com
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Abstract

The genus Narcissus L. (Amaryllidaceae) provides a model system to study the evolution and maintenance of sexual polymorphisms. In this study, we characterized microsatellite markers for N. dubius, N. cuatrecasasii, N. assoanus and N. rupicola for studies of genetic diversity and paternity analyses to investigate the stability of stylar dimorphism. We proved 40 new primer pairs from a genomic library of N. papyraceus and 12 microsatellite markers characterized also for N. papyraceus in a previous study (52 primer pairs overall). Twenty markers amplified, but their transferability and variability were different among species. Polymorphism was tested at least on 74 individuals and one population per species. The number of polymorphic loci per species ranged from four to eight. The number of alleles per locus ranged from two to 19 and the observed heterozygosity and gene diversity, from 0.107 to 0.729 and 0.103 to 0.894, respectively. These markers can be used for studies of genetic diversity and paternity analyses among individuals of N. dubius, N. cuatrecasasii, N. assoanus and N. rupicola to study the stability of stylar dimorphism.

Keywords

Narcissus dubiusN. cuatrecasasiiN. assoanusN. rupicolaAmaryllidaceaemicrosatellite
Type
Short Communication
Information
Plant Genetic Resources , Volume 17 , Issue 3 , June 2019 , pp. 285 - 288
Copyright
Copyright © NIAB 2018 

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