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An ELISA technique for the determination of soya bean lectin in animal feeds

Published online by Cambridge University Press:  22 November 2017

R.I. Prince ,
B.G. Miller ,
M. Bailey ,
E. Telemo ,
D. Patel  and
F.J. Bourne
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Extract

Soya bean (Glycine max) contains large quantities of lectin, which has been implicated as an important anti-nutritional component of animal feeds; but the significance of this has previously been difficult to assess, since no simple method has been available to accurately measure lectin concentration.

We have now developed an assay method suitable for the routine screening of animal feeds using minimal laboratory apparatus. The technique relies upon an enzyme linked immunusorbent assay (ELISA) system, to give a visible colour indication of the presence of SBL.

Samples (100 ul) are serialy diluted on microtitre plates prior coated with affinity purified rabbit anti-SBL antibody. Bound lectins are then detected using a second anti-SBL antibody raised in sheep, followed by an alkaline phosphatase conjugated pig anti (goat) IgG. The addition of p-nitrophenyl phosphate disodium (Sigma 104) substrate gives a yellow colour in the presence of lectin.

Type
General
Information
Proceedings of the British Society of Animal Production (1972) , Volume 1988 , March 1988 , pp. 88
Copyright
Copyright © The British Society of Animal Production 1988

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