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Effect of various final concentrations of glycerol in Tris and milk diluents on post-thawing survival rates of Baluchi ram spermatozoa

Published online by Cambridge University Press:  20 November 2017

Y. J. Ahangari  and
M. Nowrozi
Y. J. Ahangari
Affiliation:
University of Agricultural Science and Natural Resources, Gorgan, I R Iran E-mail:, yjahangari@yahoo.co.uk
M. Nowrozi
Affiliation:
Agricultural Research Centre, Khorasan, I R Iran
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Extract

Glycerol is the most commonly used protective substance in diluents for freezing ram semen (Salamon and Maxwell, 2000). Colas (1975) examined two concentrations of 2 and 4% glycerol for freezing ram semen and recommended an optimal level of 4% glycerol, although it may be slightly toxic to spermatozoa. The optimum concentration of glycerol for freezing ram semen is also influenced by type of diluent (Salamon and Maxwell, 2000). The objective of this study was to investigate the effect of three levels of glycerol 3, 4 and 5% and two types of diluents on motility and live rates of Baluchi ram spermatozoa.

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2004 , 2004 , pp. 186
Copyright
Copyright © The British Society of Animal Science 2004

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References

Colas, G. 1975. Effect of initial freezing temperature, addition of glycerol and dilution on the survival and fertilizing ability of deep-frozen ram semen. J. Reprod. Fert. 42:277–285.CrossRefGoogle ScholarPubMed
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