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Fibrolytic enzymes increase the hydrolysis and rate of fermentation of pure substrates in vitro

Published online by Cambridge University Press:  20 November 2017

D. Colombatto ,
F. L. Mould ,
M. K. Bhat  and
E. Owen
D. Colombatto
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, UK FMS Division, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
F. L. Mould
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, UK
M. K. Bhat
Affiliation:
FMS Division, Institute of Food Research, Colney Lane, Norwich NR4 7UA, UK
E. Owen
Affiliation:
Department of Agriculture, The University of Reading, Earley Gate, PO Box 236, Reading RG6 6AT, UK
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Extract

Treatment of forage with enzyme mixtures can increase rate of degradation in vitro (Colombatto et al., 2000a). However, the complexity of natural forage makes it difficult to determine what fractions are most affected by enzyme treatment. The use of pure substrates (e.g. cellulose and xylan), provides a way of evaluating enzyme mode of action. Therefore, the present study examined the effects of pre-treating avicel and xylan with an enzyme mixture (EM) on a) the reducing sugars produced during treatment before rumen fermentation and b) the gas production profiles during in vitro rumen fermentation using the Reading Pressure Technique (RPT) (Mauricio et al., 1999).

Type
Poster Presentations
Information
Proceedings of the British Society of Animal Science , Volume 2001 , 2001 , pp. 125
Copyright
Copyright © The British Society of Animal Science 2001

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References

Colombatto, D; Mould, F L; Bhat, M K & Owen, E. 2000a. Effects of adding fibrolytic enzymes to alfalfa stems at start of incubation, on the degradation of dry matter and fibre using the ANKOM fermentation system. Proceedings of the 3rd European Symposium on Feed Enzymes. p 48–49.Google Scholar
Colombatto, D; Mould, F L; Bhat, M K & Owen, E. 2000b. Biochemical and in vitro assessment of six enzyme preparations as potential feed additives. Proceedings of BSAS 2000, p 54.Google Scholar
Mauricio, R.M.; Mould, F. L.; Owen, E.; Channa, K. S. and Theodorou, M. K. 1999. A semi-automated in vitro gas production technique for ruminant feedstuff evaluation. Animal Feed Science and Technology, 79: 321–330.CrossRefGoogle Scholar