Distiller’s dried grains with solubles (DDGS) is a coproduct of bioethanol production from cereal grains. Due to the selective removal of starch during the production process, the protein and fibre in DDGS is approximately three times more concentrated compared with the starting material. In Europe, wheat is the main cereal used in bioethanol production and DDGS derived from this process has been traditionally fed to ruminants. Modern bioethanol plants produce DDGS with high protein quality and DDGS is therefore increasingly used in monogastric rations. However, the high fibre content remains a challenge to animal performance. An increased intestinal viscosity is the primary concern when animals are raised on diets with high levels of soluble fibres. Feed enzymes are widely used to neutralise this antinutritional effect. The major part of the dietary fibre in wheat and wheat DDGS consists of insoluble fibre, which does not cause viscosity problems, but traps valuable nutrients in the feed matrix. At least a partial degradation of this fibre is required to release the nutrients in the gastrointestinal tract. Fibre fermentation in the hindgut of monogastric animals may represent a significant energy salvage due to the production of volatile fatty acids and contribute to the maintenance of the intestinal health status. This paper presents an in vitro comparison of the functionality of fibre from both whole wheat and wheat DDGS in monogastric nutrition, and shows how feed enzymes may improve the nutritional quality of DDGS.