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Stress responses of tomato fruit tissue submitted to massive doses of ionising radiation

Published online by Cambridge University Press:  05 December 2011

C. Triantaphylidès ,
N. Banzet ,
J. M. Ferullo ,
C. Larrigaudière  and
L. Nespoulous
C. Triantaphylidès*
Affiliation:
Département de Physiologie Végétale et Ecosystemes, Section de Radiophysiologie Végétale, Commissariat à L'Energie Atomique, Centre de Cadarache, 13108 Saint-Paul-lez-Durance Cedex, France
N. Banzet
Affiliation:
Département de Physiologie Végétale et Ecosystemes, Section de Radiophysiologie Végétale, Commissariat à L'Energie Atomique, Centre de Cadarache, 13108 Saint-Paul-lez-Durance Cedex, France
J. M. Ferullo
Affiliation:
Département de Physiologie Végétale et Ecosystemes, Section de Radiophysiologie Végétale, Commissariat à L'Energie Atomique, Centre de Cadarache, 13108 Saint-Paul-lez-Durance Cedex, France
C. Larrigaudière
Affiliation:
Département de Physiologie Végétale et Ecosystemes, Section de Radiophysiologie Végétale, Commissariat à L'Energie Atomique, Centre de Cadarache, 13108 Saint-Paul-lez-Durance Cedex, France
L. Nespoulous
Affiliation:
Département de Physiologie Végétale et Ecosystemes, Section de Radiophysiologie Végétale, Commissariat à L'Energie Atomique, Centre de Cadarache, 13108 Saint-Paul-lez-Durance Cedex, France
*
*To whom correspondence should be addressed.
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Abstract

In plant tissue, massive doses of ionising radiation (0.5–3 kGy) induce an oxidative burst due to the overproduction of oxygen-centred free radicals. Changes in the protein metabolism of cherry tomato fruits were investigated in response to this peculiar stress. Although DNA damage definitively arrested cell division, the changes observed on a short-term basis were attributed to genetic regulation. Changes in protein metabolism were also maintained long term. Gamma-induced proteins (GIPs) were classified according to their induction kinetics. Group 1 proteins were induced immediately after the treatment and their synthesis was stopped within 24 h. During the same time period, global protein synthesis was restored and a new set of GIPs was induced. The function of these proteins is not yet known; but they may be involved in physiological disorders triggered by irradiation or in repair processes. Short-term typical changes involve the synthesis of ACC synthase – the ethylene pathway regulating enzyme - and most probably of some LMW-HSPs. A non-relevant response to irradiation has also been discovered, namely the long-term accumulation of chitinases. Irradiation induces both specific and non-specific responses which can be analysed by comparison with other types of oxidative stress and some GIPs seem to be specific to the treatment. The ability of irradiation to induce such different responses might be profitably applied for a better understanding of the oxidative mechanisms involved in signal transduction during environmental stress situations.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 102: Oxygen and Environmental Stress in Plants , 1994 , pp. 97 - 105
Copyright
Copyright © Royal Society of Edinburgh 1994

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Footnotes

1Current address: Dept. STA, Faculté des Sciences de l'Agriculture et de l'Alimentation, Université Laval, Québec Canada, G1K 7P4.
2Current address: IRTA-UPC Alcade Rovira Roure 177, 25006 Lleida, Spain.

References

Abdel-Kader, A. S., Mooris, L. L. & Maxie, E. C. 1968. Physiological studies of gamma irradiated tomato fruits. I: Effects on respiratory rate, ethylene production and ripening. Proceedings of the American Society for Horticultural Science 92, 553–67.Google Scholar
Akamine, E. K. & Goo, T. 1971. Respiration of gamma-irradiated fresh fruits. Journal of Food Science 36, 1074–7.CrossRefGoogle Scholar
Boller, T. 1993. Antimicrobial functions of plant hydrolases, chitinase and β-1,3-glucanase. In Fritig, B. & Legrand, M. (Eds) Mechanisms of plant defense responses, pp. 391–400. Dordrecht: Kluwer Academic Publ.CrossRefGoogle Scholar
Brady, C. J. 1987. Fruit ripening. Annual Review of Plant Physiology 38 155–78.CrossRefGoogle Scholar
Broglie, R. & Broglie, K. 1993. Chitinases and plant protection. In Fritig, B. & Legrand, M. (Eds) Mechanisms of plant defense responses, pp. 411–21. Dordrecht: Kluwer Academic Publ.CrossRefGoogle Scholar
Casarett, A. P. 1968. Radiation chemistry and effects of gamma radiation on the cell. In Casarett, A. P. (Ed.) Radiation biology. Englewood Cliffs, NJ: Prentice-Hall.Google Scholar
Chagvardieff, P., Dimon, B., Carrier, P. & Triantaphylidès, C. 1989. Cell division arrest by gammairradiation in photoautotrophic suspension culture of Euphorbia characias: maintenance of photosynthetic capacity and overaccumulation of sucrose. Plant Cell, Tissue and Organ Culture 19, 141–9.CrossRefGoogle Scholar
Collinge, D. B., Kragh, K. M., Mikkelsen, J. D., Nielsen, K. K., Rasmussen, U. & Vad, K. 1993. Plant chitinases. The Plant Journal 3, 31–40.CrossRefGoogle ScholarPubMed
Conyers, S. M. & Kidwell, D. A. 1991. Chromogenic substrates for horseradich peroxidase. Analytical Biochemistry 192, 207–11.CrossRefGoogle ScholarPubMed
Elstner, E. F. 1991. Mechanisms of oxygen activation in different compartments of plants. In Pell, E. & Steffen, K. (Eds) Active oxygen/oxidative stress and plant metabolism, pp. 13–25. Rockville: Amer. Soc. Plant Physiol.Google Scholar
Elstner, E. F., Wagner, G. A. & Schutz, W. 1988. Activated oxygen in green plants in relation to stress situations. Current Topics in Plant Biochemistry and Physiology 7, 159–87.Google Scholar
Farr, S. B. & Kogoma, T. 1991. Oxidative stress responses in Escherichia coli and Salmonella tiphymurium. Microbiological Reviews 55, 561–85.CrossRefGoogle Scholar
Ferullo, J. M. & Nespoulous, L. 1991. Two-dimensional electrophoresis of plant proteins with Phastsystem using nonequilibrium pH gradient separation. Analytical Biochemistry 198, 131–3.CrossRefGoogle ScholarPubMed
Ferullo, J. M., Montoya, N. & Triantaphylides, C. 1993. Role of ethylene in the differential induction of acidic, neutral and basic chitinases in pericarp of irradiated cherry tomato fruits. In Fritig, B. & Legrand, M. (Eds) Mechanisms of plant defense responses, p. 178. Dordrecht: Kluwer Academic Publ.CrossRefGoogle Scholar
Ferullo, J. M., Nespoulous, L. & Triantaphylides, C. 1994. Gamma ray-induced changes in protein synthesis of tomato pericarp. Plant Cell and Environment 17 (in press).CrossRefGoogle Scholar
Frylink, L., Dubery, I. A. & Schabort, J. C. 1987. Biochemical changes involved in stress response and ripening behaviour of gamma irradiated mango fruit. Phytochemistry 26, 681–6.Google Scholar
Gidrol, X., Kogoma, T. & Farr, S. B. 1992. Oxidative stress responses in bacteria. In: Encyclopedia of Microbiology, Vol. 3, pp. 315–26. San Diego: Academic Press.Google Scholar
Iacazio, G., Langrand, G., Baratti, J., Buono, G. & Triantaphylidès, C. 1990. Preparative, enzymatic synthesis of linoleic acid (13S)-hydroperoxide using soybean lipoxygenase-1. Journal of Organic Chemistry 55, 1690–1.CrossRefGoogle Scholar
Inoue, M. & van Huystee, R. B. 1984. Age-dependent effects of gamma-exposure on form, growth and peroxidase activity of cultured peanut cells. Environmental and Experimental Botany 20, 161–8.CrossRefGoogle Scholar
Kader, A. A. 1986. Potential applications of ionizing radiation in postharvest handling of fresh fruits and vegetables. Food Technology 40, 117–21.Google Scholar
Ku, L. L. & Romani, R. J. 1970. The ribosomes of pear fruit. Their synthesis during the climacteric and the age-related compensatory response to ionizing radiation. Plant Physiology 45, 401–7.CrossRefGoogle ScholarPubMed
Larrigaudière, C., Latché, A., Pech, J. C. & Triantaphylidès, C. 1990. Short-term effects of gammairradiation on 1-aminocyclopropane-l-carboxylic acid metabolism in early climacteric cherry tomatoes. Plant Physiology 92, 577–81.CrossRefGoogle Scholar
Larrigaudière, C., Latché, A., Pech, J. C. & Triantaphylidès, C. 1991. Relationship between stress ethylene production induced by gamma irradiation and ripening of cherry tomatoes. Journal of American Society for Horticultural Science 116, 1000–3.CrossRefGoogle Scholar
Lee, T. H., McGlasson, W. B. & Edwards, R. A. 1968. Effect of gamma radiation on tomato fruit picked at four stages of development. Radiation Botany 8, 259–67.CrossRefGoogle Scholar
McGlasson, W. B. & Lee, T. H. 1971. Damage and repair of protein in gamma irradiated tomato fruit. Radiation Biology 11, 239–41.CrossRefGoogle Scholar
McGlasson, W. B. 1985. Ethylene and fruit ripening. Horticultural Science 20, 51–4.Google Scholar
Meyer, Y., Grosset, J., Chartier, Y. & Cleyet-Marel, J.-C. 1988. Preparation by two-dimensional electrophoresis of proteins for antibody production: antibodies against proteins whose synthesis is reduced by auxin in tobacco mesophyll protoplasts. Electrophoresis 9, 704–12.CrossRefGoogle ScholarPubMed
Pendharkar, M. B. & Nair, P. M. 1975. Induction of phenylalanine ammonia-lyase (PAL) in gamma irradiated potatoes. Radiation Botany 15, 191–7.CrossRefGoogle Scholar
Riov, J. & Goren, R. 1970. Effect of gamma radiation and ethylene on protein synthesis in peel of mature grapefruit. Radiation Botany 10, 155–60.CrossRefGoogle Scholar
Riov, J., Monselise, S. P. & Kalian, R. S. 1970. Radiation damage to grape fruit in relation to ethylene production and phenylalanine ammonia-lyase activity. Radiation Botany 10, 281–6.CrossRefGoogle Scholar
Romani, R. J. 1966. Biochemical responses of plant systems to large doses of ionizing radiation. Radiation Botany 6, 87–104.CrossRefGoogle Scholar
Romani, R. J. 1984. Respiration, ethylene, senescence, and homeostasis in an integrated view of postharvest life. Canadian Journal of Botany 62, 2950–5.CrossRefGoogle Scholar
Scandalios, J. G. 1993. Oxygen stress and superoxide dismutases. Plant Physiology 101, 7–12.CrossRefGoogle ScholarPubMed
Urbain, W. M. 1986. Fruits, vegetables and nuts. In Schweigert, B. S. (Ed.) Food irradiation, pp. 170–216. New York: Academic Press.CrossRefGoogle Scholar
Verma, D. P. S. & van Huystee, R. B. 1971. Aberrant recovery of protein synthesis after massive irradiation of Arachis hypogaea, L. cells in vitro. Radiation Research 48, 531–41.CrossRefGoogle Scholar
von Sonntag, C. 1987. In von Sountag, C. (Ed.) The chemical basis of radiation biology, pp. 31–56. London: Taylor & Francis.Google Scholar
Yang, S. F. & Hoffman, E. 1984. Ethylene biosynthesis and its regulation in higher plants. Annual Review of Plant Physiology 35, 155–89.CrossRefGoogle Scholar
Young, R. E. 1965. Effect of ionising radiation on respiration and ethylene production of avocado fruit. Nature 205, 1113–4.CrossRefGoogle ScholarPubMed