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XII.—The Metabolism of Sodium Bromosulphthalein in the Fowl*

Published online by Cambridge University Press:  11 June 2012

J. G. Campbell
J. G. Campbell
Affiliation:
British Empire Cancer Campaign Unit, Poultry Research Centre, Edinburgh, 9
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Synopsis

Evidence is presented that, in fowls, as in a number of mammals, intravenously administered sodium bromosulphthalein is excreted by the liver into the bile in several forms. These metabolites are not glucuronides, esters or sulphates, and in the fowl do not have the chromatographic mobilities or other characteristics of any of the amino acids described as conjugating with BSP in mammals. In all, three forms of BSP are detectable in fowl bile after administration of the dye, one, with the highest Rf. is unchanged BSP, the others are associated with ninhydrin positive material, and have lower Rf. values. Conjugation has been studied in young chickens and in adults, and the effects of sex hormones, cortisone and liver damage have been assessed. It is shown that a proportion of the injected BSP may be retained for some time in the liver as a colourless form, so that only prolonged alkaline hydrolysis of liver extract and homogenate results in its liberation as a detectable coloured form. The significance of this, and the effect of sex hormones in influencing the amount of conjugation and thus possibly affecting the rate of liver clearance of BSP is discussed, and evidence is presented that there exists an apparent inverse relationship between the amount of œstrogen as assessed by BSP liver-clearance test and the relative proportions of the two BSP conjugates in the bile. Also direct relationship may exist between the amount of œstrogen and the proportion of colourless BSP found in the liver.

Type
Research Article
Information
Proceedings of the Royal Society of Edinburgh, Section B: Biological Sciences , Volume 67 , Issue 4 , 1960 , pp. 319 - 332
Copyright
Copyright © Royal Society of Edinburgh 1960

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Footnotes

*

This paper was assisted in publication by a grant from the Carnegie Trust for the Universities of Scotland.

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