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Applications of Pulse Radiolysis to Protein Chemistry

Published online by Cambridge University Press:  17 March 2009

M. H. Klapper  and
M. Faraggi
M. H. Klapper
Affiliation:
Ohio State University, Columbus, Ohio, U.S.A., and Nuclear Research Centre-Negev, Beer- Sheva, Israel
M. Faraggi
Affiliation:
Ohio State University, Columbus, Ohio, U.S.A., and Nuclear Research Centre-Negev, Beer- Sheva, Israel
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Extract

Since its introduction, pulse radiolysis has been an important technique for examining the properties of organic and inorganic radicals, and for enumerating those reactions responsible for cellular damage by ionizing radiation. Biochemists, and biophysicists outside the area of radiation biology appear, perhaps for historical reasons, to have an incomplete appreciation of the technique's potential. Protein chemists in particular, have been only dimly aware of the numerous reports of, and the significant results obtained from pulse radiolysis studies of proteins. Our purpose here is to bring some of these results together in order to emphasize the power and usefulness of pulse radiolysis experiments both for elucidating enzyme reaction mechanisms, and for gaining information on the structure of proteins in aqueous solutions. Reviews containing related, or in part the same material to be covered here have appeared previously; for example, Land (1970), Adams et al. (1972a), Shafferman & Stein (1975), Adams & Wardman (1977). This review updates these earlier works, but more importantly approaches the topic of protein pulse radiolysis with a different emphasis.

Type
Articles
Information
Quarterly Reviews of Biophysics , Volume 12 , Issue 4 , November 1979 , pp. 465 - 519
Copyright
Copyright © Cambridge University Press 1979

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