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Some recent applications of the use of paramagnetic centres to probe biological systems using nuclear magnetic resonance

Published online by Cambridge University Press:  17 March 2009

Arabella T. Morris  and
Raymond A. Dwek
Arabella T. Morris
Affiliation:
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU
Raymond A. Dwek
Affiliation:
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX13QU
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Extract

The explosion of the use of paramagnetic probes to study biological problems by nuclear magnetic resonance (n.m.r.) stems from the large and easily quantifiable perturbations they produce. The main reason for these perturbations is that the magnetic moments of unpaired electrons are about 103 times greater than nuclear magnetic moments and consequently they generate much greater local fields.Such fields can give rise to large shifts in nuclear resonances; but if these fields fluctuate at the appropriate frequencies they will also cause very efficient relaxation in the nuclear resonances. It is possible to select probes that result mainly in one of these parameters being perturbed so that the terms shift and relaxation probe are now well established in the literature.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 10 , Issue 4 , November 1977 , pp. 421 - 484
Copyright
Copyright © Cambridge University Press 1977

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