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Voltage clamp and tracer flux data: effects of a restricted extra-cellular space

Published online by Cambridge University Press:  17 March 2009

David Attwell ,
David Eisner  and
Ira Cohen
David Attwell
Affiliation:
University Laboratory of Physiology, Parks Road, Oxford, England, and Department of Physiology & Biophysics, SUNY at Stony Brook, NY 11794, U.S.A.
David Eisner
Affiliation:
University Laboratory of Physiology, Parks Road, Oxford, England, and Department of Physiology & Biophysics, SUNY at Stony Brook, NY 11794, U.S.A.
Ira Cohen
Affiliation:
University Laboratory of Physiology, Parks Road, Oxford, England, and Department of Physiology & Biophysics, SUNY at Stony Brook, NY 11794, U.S.A.
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Extract

In the original Hodgkin & Huxley analysis of the membrane currents in squid giant axon, the time dependence of the currents was attributed to voltage-dependent changes in the permeability of the membrane to the ions involved. According to this theoretical framework, the time constants for the rate of change of current should be unique functions of the membrane potential, and should be independent of the previous history of the voltage and current flowing. In recent years, however, there has been increasing awareness of the fact that, for many physiological preparations, the space just outside the cell membrane is not in good diffusive contact with the bulk extra-cellular fluid perfusing the preparation. Consequently, when current flows across the cell membrane, there are changes in the ion concentrations in this so-called ‘restricted extra-cellular space’ (RECS).

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 12 , Issue 3 , August 1979 , pp. 213 - 261
Copyright
Copyright © Cambridge University Press 1979

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