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NMR studies of enzymatic rates in vitro and in vivo by magnetization transfer

Published online by Cambridge University Press:  17 March 2009

J. R. Alger  and
R. G. Shulman
J. R. Alger
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven CT 06511
R. G. Shulman
Affiliation:
Department of Molecular Biophysics and Biochemistry, Yale University, New Haven CT 06511
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Extract

Magnetization transfer techniques are specialized NMR experiments which can measure the rate of chemical reactions while concentrations of products and reactants are maintained constant. These techniques are being used to measure the rates of enzyme catalysed reactions in a variety of living systems and in vitro. The magnetization transfer measurements in vivo of the ATP synthetase and the creatine kinase reactions have been particularly useful in describing rates of major energy transducing reactions involving ATP and phosphocreatine. As a result, a wide range of biomedicai scientists are becoming aware of the potentials of these techniques. The purpose of this review is thus threefold: first, to present a concise, conceptual review of the underlying principles for these non-specialists; secondly, to review the important biochemical applications of the method which have appeared, and thirdly, to discuss potential applications and limitations of the method.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 17 , Issue 1 , February 1984 , pp. 83 - 124
Copyright
Copyright © Cambridge University Press 1984

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