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Recommendations for measurement and presentation of biochemical equilibrium data*

Prepared by the Interunion Commission on Biothermodynamics†

Published online by Cambridge University Press:  17 March 2009

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Abstract

Equilibrium data are of importance in describing and understanding of biochemical systems. At present there is marked variation among different investigators in the choice of experimental conditions for the study of similar or identical reactions and in the manner of reporting the data. In many cases the description of the system does not provide all the essential information that would permit reproduction of the experiments. This can lead to confusion and difficulties in correlating the results of different workers.

Equilibrium studies in biochemistry involve special problems that are not encountered in general chemistry. The attainment of biochemical equilibria commonly involves the addition of a specific enzyme to the system to catalyse the reaction studied; sometimes two or more enzymes must be added. In addition the enzyme may require the presence of certain cofactors, such as metal ions. The reactants, or products, or both, may bind or release protons or other ions during the reaction under study.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 9 , Issue 3 , August 1976 , pp. 439 - 456
Copyright
Copyright © Cambridge University Press 1976

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References

REFERENCES

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