Hostname: page-component-78c5997874-s2hrs Total loading time: 0 Render date: 2024-11-19T09:14:00.638Z Has data issue: false hasContentIssue false
  • English
  • Français

Contrasting tocol ratios associated with seed longevity in rice variety groups

Published online by Cambridge University Press:  10 October 2017

Jae-Sung Lee ,
Jieun Kwak ,
Mi-Ra Yoon ,
Jeom-Sig Lee  and
Fiona R. Hay
Jae-Sung Lee
Affiliation:
T. T. Chang Genetic Resources Center, International Rice Research Institute, Los Baños, College, Laguna, 4031, Philippines
Jieun Kwak
Affiliation:
National Institute of Crop Science, Rural Development Administration, Suwon, Gyunggi-do, Republic of Korea
Mi-Ra Yoon
Affiliation:
National Institute of Crop Science, Rural Development Administration, Suwon, Gyunggi-do, Republic of Korea
Jeom-Sig Lee
Affiliation:
National Institute of Crop Science, Rural Development Administration, Suwon, Gyunggi-do, Republic of Korea
Fiona R. Hay*
Affiliation:
T. T. Chang Genetic Resources Center, International Rice Research Institute, Los Baños, College, Laguna, 4031, Philippines
*
*Correspondence Email: f.hay@irri.org
Get access Rights & Permissions [Opens in a new window]

Abstract

Vitamin E (tocols) is a key metabolite for efficient scavenging of lipid peroxy radicals that cause membrane breakdown during seed ageing. However, in rice, this hypothesis has been tested for very few lines only and without considering intraspecific variation in genomic structure. Here, we present a correlation study between tocols and seed longevity using a diverse rice panel. Seeds of 20 rice accessions held in the International Rice Genebank at the International Rice Research Institute, representing Aus, Basmati/Sadri, Indica, temperate Japonica and tropical Japonica variety groups, were used for tocols analysis (quantification of α-, β-, γ- and δ-tocopherol/tocotrienol by ultra-performance liquid chromatography) and storage experiments at 45°C and 10.9% seed moisture content. To examine the effects of DNA-haplotype on the phenotype, the 700 K high-density single-nucleotide polymorphism marker dataset was utilized. Both seed longevity (time for viability to fall to 50%; p50) and tocols content varied across variety groups related to the heterogeneity in the genetic architecture. Among eight types of tocol homologues, α-tocopherol and γ-tocotrienol were significantly correlated with p50 (negatively and positively, respectively). While temperate Japonica varieties were most abundant in α-tocopherol, Indica varieties recorded 1.3- to 1.7-fold higher γ-tocotrienol than those of other groups. We conclude that the specific ratio of tocol homologues rather than total tocols content plays an important role in the seed longevity mechanism.

Keywords

riceseed longevitytocoltocopherol
Type
Research Papers
Information
Seed Science Research , Volume 27 , Issue 4 , December 2017 , pp. 273 - 280
Check for updates
Copyright
Copyright © Cambridge University Press 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

†

First authors

References

Aggarwal, B.B., Sundaram, C., Prasad, S. and Kannappan, R. (2010) Tocotrienols, the vitamin E of the 21st century: its potential against cancer and other chronic diseases. Biochemical Pharmacology 80, 1613–1631.Google Scholar
Chen, D., Li, Y., Fang, T., Shi, X. and Chen, X. (2015) Specific roles of tocopherols and tocotrienols in seed longevity and germination tolerance to abiotic stress in transgenic rice. Plant Science 244, 31–39.CrossRefGoogle ScholarPubMed
Goufo, P. and Trindade, H. (2013) Rice antioxidants: phenolic acids, flavonoids, anthocyanins, proanthocyanidins, tocopherols, tocotrienols, c-oryzanol, and phytic acid. Food Science and Nutrition 2, 75–104.Google Scholar
Hameed, A., Rasheed, A., Gul, B. and Khan, M.A. (2014) Salinity inhibits seed germination of perennial halophytes Limonium stocksii and Suaeda fruticosa by reducing water uptake and ascorbate dependent antioxidant system. Environmental and Experimental Botany 107, 32–38.Google Scholar
Hay, F.R. and Probert, R.J. (1995) The effect of different drying conditions and maturity on desiccation tolerance and seed longevity in Digitalis purpurea L. Annals of Botany 76, 639–647.Google Scholar
Hay, F.R. and Probert, R.J. (2013) Advances in seed conservation of wild plant species: a review of recent research. Conservation Physiology 1, 1–11.CrossRefGoogle ScholarPubMed
Hay, F.R., Timple, S. and van Duijn, B. (2015) Can chlorophyll fluorescence be used to determine the optimal time to harvest rice seeds for long-term genebank storage? Seed Science Research 25, 321–334.Google Scholar
Jiang, Q. (2014) Natural forms of vitamin E: metabolism, antioxidant, and anti-inflammatory activities and their role in disease prevention and therapy. Free Radical Biology and Medicine 72, 76–90.Google Scholar
Kadoma, Y., Ishihara, M., Okada, N. and Fujisawa, S. (2006) Free radical interaction between vitamin E (α-, β-, γ- and δ-tocopherol), ascorbate and flavonoids. In Vivo 20, 823–828.Google Scholar
Kamal-Eldin, A. and Appelqvist, L.A. (1996) The chemistry and antioxidant properties of tocopherols and tocotrienols. Lipids 31, 671–701.CrossRefGoogle ScholarPubMed
Kim, N.H., Kwak, J., Baik, J.Y., Yoon, M.R., Lee, J.S., Yoon, S.W. and Kim, I.H. (2015) Changes in lipid substances in rice during grain development. Phytochemistry 116, 170–179.Google Scholar
Kim, H.J (2014) Effect of α-, β-, γ-, and δ-tocotrienol on the oxidative stability of lard. Journal of the American Oil Chemists’ Society 91, 777–782.Google Scholar
Ko, S.N., Kim, C.J., Kim, H., Kim, C.T., Chung, S.H., Tae, B.S. and Kim, I.H. (2003) Tocol levels in milling fractions of some cereal grains and soybean. Journal of the American Oil Chemists’ Society 80, 585–589.Google Scholar
Liebler, D.C. (1993) The role of metabolism in the antioxidant function of vitamin E. Critical Reviews in Toxicology 23, 147–169.Google Scholar
Lehner, A., Mamadou, N., Poels, P., Côme, D., Bailly, C. and Corbineau, F. (2008) Changes in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activities in the embryo during ageing in wheat grains. Journal of Cereal Science 47, 555–565.Google Scholar
Lushchak, V.I. and Semchuk, N.M. (2012) Tocopherol biosynthesis: chemistry, regulation and effects of environmental factors. Acta Physiologiae Plantarum 34, 1607–1628.Google Scholar
McCouch, S.R., Wright, M.H., Tung, C.-W., Maron, L.G., McNally, K.L., Fitzgerald, M., Singh, N., DeClerck, G., Agosto-Perez, F., Korniliev, P., Greenberg, A.J., Naredo, M.E.B., Mercado, S.M.Q., Harrington, S.E., Shi, Y., Branchini, D.A., Kuser-Falcaõ, P.R., Leung, H., Ebana, K., Yano, M., Eizenga, G., McClung, A. and Mezey, J. (2016) Open access resources for genome-wide association mapping in rice. Nature Communications; doi: 10.1038/ncomms10532 Google ScholarPubMed
McNally, K.L., Childs, K.L., Bohnert, R., Davidson, R.M., Zhao, K., Ulat, V.J., Zeller, G., Clark, R.M., Hoen, D.R., Bureau, T.E., Stokowski, R., Ballinger, D.G., Frazer, K.A., Cox, D.R., Padhukasahasram, B., Bustamante, C.D., Weigel, D., Mackill, D.J., Bruskiewich, R.M., Ratsch, G., Buell, C.R., Leung, H. and Leach, J.E. (2009) Genomewide SNP variation reveals relationships among landraces and modern varieties of rice. Proceedings of the National Academy of Sciences of the United States of America 106, 12273–12278.Google Scholar
Nagel, M., Kranner, I., Neumann, K., Rolletschek, H., Seal, C.E., Colville, L., Fernández-Marín, B. and Börner, A. (2015) Genome-wide association mapping and biochemical markers reveal that seed ageing and longevity are intricately affected by genetic background and developmental and environmental conditions in barley. Plant, Cell and Environment 38, 1011–1022.Google Scholar
Noctor, G. and Foyer, C.G. (1998) Ascorbate and glutathione: keeping active oxygen under control. Annual Review of Plant Physiology and Plant Molecular Biology 49, 249–279.Google Scholar
Probert, R., Adams, J., Coneybeer, J., Crawford, A. and Hay, F. (2007) Seed quality for conservation is critically affected by pre-storage factors. Australian Journal of Botany 55, 326–355.Google Scholar
Sattler, S.E., Cahoon, E.B., Coughlan, S.J. and DellaPenna, D. (2003) Characterization of tocopherol cyclases from higher plants and cyanobacteria. evolutionary implications for tocopherol synthesis and function. Plant Physiology 132, 2184–2195.CrossRefGoogle ScholarPubMed
Sattler, S.E., Gilliland, L.U., Magallanes-Lundback, M., Pollard, M. and DellaPenna, D. (2004) Vitamin E is essential for seed longevity and for preventing lipid peroxidation during germination. The Plant Cell 16, 1419–1432.Google Scholar
The 3,000 Rice Genomes Project (2014) The 3,000 rice genomes project. Giga Science 3, 7.Google Scholar
Traber, M.G. and Stevens, J.F. (2011) Vitamins C and E: beneficial effects from a mechanistic perspective. Free Radical Biology and Medicine 51, 1000–1013.Google Scholar
Whitehouse, K.J., Hay, F.R. and Ellis, R.H. (2015) Increases in the longevity of desiccation-phase developing rice seeds: response to high-temperature drying depends on harvest moisture content. Annals of Botany 116, 247–259. doi: 10.1093/aob/mcv091 Google Scholar
Wang, X., Song, Y.-E. and Li, J.-Y. (2013) High expression of tocochromanol biosynthesis genes increases the vitamin E level in a new line of giant embryo rice. Journal of Agricultural and Food Chemistry 61, 5860–5869.Google Scholar
Zhao, K., Tung, C.-W., Eizenga, G.C., Wright, M.H., Ali, M.L., Price, A.H., Norton, G.J., Islam, M.R., Reynolds, A., Mezey, J., Mcclung, A.M., Bustamante, C.D. and McCouch, S.R. (2011) Genome-wide association mapping reveals a rich genetic architecture of complex traits in Oryza sativa . Nature Communication; doi: 10.1038/ncomms1467 Google Scholar
Supplementary material: File

Lee et al supplementary material

Table S1

Download Lee et al supplementary material(File)
File 38.7 KB
Supplementary material: File

Lee et al supplementary material

Table S1

Download Lee et al supplementary material(File)
File 40.1 KB
Supplementary material: File

Lee et al supplementary material

Figure S1

Download Lee et al supplementary material(File)
File 133.1 KB
Supplementary material: File

Lee et al supplementary material

Figure S1

Download Lee et al supplementary material(File)
File 135.6 KB