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The effect of aerated hydration on DNA synthesis in embryos of Brassica oleracea L.

Published online by Cambridge University Press:  19 September 2008

J. M. Thornton ,
A. R. S. Collins  and
A. A. Powell
J. M. Thornton*
Affiliation:
Department of Biological Sciences, Heriot-Watt University, Riccarton, Edinburgh EH14 4AS, UK
A. R. S. Collins
Affiliation:
Rowett Research Institute, Greenburn Road, Aberdeen AB2 9SB, UK
A. A. Powell
Affiliation:
Department of Agriculture, University of Aberdeen, Aberdeen AB9 1UD, UK
*
* Correspondence
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Abstract

Ageing causes a delay in the onset of DNA replication in seeds. Aerated hydration for 8 h, a treatment to invigorate seeds, resulted in a reduction of about 12 h in the time difference in the onset of DNA synthesis between unaged and aged embryos of brassica seed. This effect is consistent with a recovery process occurring during aerated hydration of the seed involving the repair of accumulated DNA damage. The occurrence of hydroxyurea-resistant 3H-thymidine incorporation in aged Brussels sprouts embryos during the period 16–32 h from the start of hydration supports this interpretation.

Keywords

BrassicaDNA repairreplication
Type
Research Papers
Information
Seed Science Research , Volume 3 , Issue 3 , September 1993 , pp. 195 - 199
Copyright
Copyright © Cambridge University Press 1993

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