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Rapid and efficient purification of seed messenger RNA without phenol:chloroform extraction

Published online by Cambridge University Press:  19 September 2008

S. K. Pramanik ,
T. L. Reynolds ,
S. A. Maclsaac  and
J. D. Bewley
S. K. Pramanik*
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
T. L. Reynolds
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
S. A. Maclsaac
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
J. D. Bewley
Affiliation:
Department of Botany, University of Guelph, Guelph, Ontario, N1G 2W1, Canada
*
* Correspondence
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Abstract

A novel method has been devised to extract poly (A+)RNA from seed tissues. The advantages of this technique include the speed of extraction and the elimination of the hazardous phenol:chloroform extraction step which usually precedes oligo (dT)-cellulose column chromatography. Instead, the crude supernatant, containing a high concentration of SDS and salts to effectively inhibit RNase activity, is passed directly over the oligo (dT)-cellulose column. The resultant isolated poly (A+)RNA is of high yield and purity and is suitable for in vitro translation and Northern blot analysis.

Keywords

seed messenger RNApoly (A+)RNA extraction technique
Type
Short Communication
Information
Seed Science Research , Volume 3 , Issue 2 , June 1993 , pp. 137 - 139
Copyright
Copyright © Cambridge University Press 1993

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References

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