Hostname: page-component-77c89778f8-vsgnj Total loading time: 0 Render date: 2024-07-21T17:26:11.192Z Has data issue: false hasContentIssue false
  • English
  • Français

The relationship between the mitotic activity and moisture content of recalcitrant seeds of Acer saccharinum (L.) during maturation, post-maturation drying and germination

Published online by Cambridge University Press:  22 February 2007

L.E. Kozeko  and
V.M. Troyan
L.E. Kozeko*
Affiliation:
N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska 2, 252601 Kiev, Ukraine
V.M. Troyan
Affiliation:
N.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Tereshchenkivska 2, 252601 Kiev, Ukraine
*
*Correspondence Email: rochem@ukrpack.net
Get access Rights & Permissions [Opens in a new window]

Abstract

The decline of embryo moisture content from approx. 82 to 53% in 1997 and 56% in 1998 in recalcitrant seeds of Acer saccharinum during maturation was accompanied by decreased mitotic activity in the meristems and an increase in the percentage of cells in the G1 phase of the cell cycle. DNA synthesis and mitosis in the root apex ceased at approx. 53% embryo moisture content, and 67% of the cells were arrested in the G1 phase. During post-maturation drying, cell division in the shoot apex and embryonic leaves continued as long as the embryo moisture content was higher than 50 and 45%, respectively. Mitotic activity in the drying embryo may be controlled by its moisture level. Increased proliferation of the root, shoot and leaf meristems of the mature seeds was already recorded at 24 h of germination, before the root protruded through the seed coat. However, the increase in the embryo moisture content was small – from 56 to 59%. In the 3 d seedlings (10–15 mm long) the mitotic index reached 8% in the root apex and 12% in the shoot apex with leaves. Placing mature seeds in moist conditions might be necessary for the switch from proliferation decline towards its activation. Thus, in contrast with orthodox seeds, the preservation of cell division capacity and increased mitotic activity may be essential for rapid germination immediately after shedding in mature Acer seeds.

Keywords

Acer saccharinumdormancyElymus elymoidesgerminationmaturationmitotic activitymoisture contentrecalcitrant seed
Type
Research Article
Information
Seed Science Research , Volume 10 , Issue 3 , September 2000 , pp. 225 - 232
Copyright
Copyright © Cambridge University Press 2000

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Avanzi, S., Brunori, A. and D'Amato, F. (1969) Sequential development of meristems in the embryo of Triticum durum. A DNA autoradiographic and cytophotometric analysis. Developmental Biology 20, 368–377.CrossRefGoogle ScholarPubMed
Arnason, T.J., El-Sadek, L.M. and Minocha, J.L. (1966) Effects of some variations of treatment-methods on mutation frequency in barley treated with some monofunctional alkylating agents. Canadian Journal of Genetics and Cytology 8, 746–755.CrossRefGoogle Scholar
Berjak, P., Farrant, J.M. and Pammenter, N.W. (1989) The basis of recalcitrant seed behaviour. pp. 89–108in Taylorson, R.B. (Ed.) Recent advances in the development and germination of seeds. New York, Plenum Press.CrossRefGoogle Scholar
Bewley, J.D. and Black, M. (1994) Seeds. Physiology of development and germination (2nd edition). pp. 35–197, New York, Plenum Press.CrossRefGoogle Scholar
Bino, R.J., Lanteri, S., Verhoeven, H.A. and Kraak, H.L. (1993) Flow cytometric determination of nuclear replication stages in seed tissues. Annals of Botany 72, 181–187.CrossRefGoogle Scholar
Brunori, A. (1967) Relationship between DNA synthesis and water content during ripening of Vicia faba seeds. Caryologia 20, 333–338.CrossRefGoogle Scholar
Chin, H.F., Hor, Y.L. and Lassim, M.B.M. (1984) Identification of recalcitrant seeds. Seed Science and Technology 12, 429–436.Google Scholar
Crèvecoeur, M., Deltour, R. and Bronchart, R. (1976) Cytological study on water stress during germination of Zea mays. Planta 132, 31–41.CrossRefGoogle Scholar
Deltour, R. (1985) Nuclear activation during early germination of the higher plant embryo. Journal of Cell Science 75, 43–83.CrossRefGoogle ScholarPubMed
Deltour, R. and Jacqmard, A. (1974) Relation between water stress and DNA synthesis during germination of Zea mays L. Annals of Botany 38, 529–534.CrossRefGoogle Scholar
Elder, R.H. and Osborne, D.J. (1993) Function of DNA synthesis and DNA repair in the survival of embryos during early germination and in dormancy. Seed Science Research 3, 43–53.CrossRefGoogle Scholar
Finch-Savage, W.E., Bergervoet, J.H.W., Bino, R.J., Clay, H.A. and Groot, S.P.C. (1998) Nuclear replication activity during seed development, dormancy breakage and germination in three tree species: Norway maple (Acer platanoides L.), sycamore (Acer pseudoplatanus L.) and cherry (Prunus avium L.). Annals of Botany 81, 519–526.CrossRefGoogle Scholar
Hamada, S. and Fujita, S. (1983) DAPI staining improved for quantitative cytofluorometry. Histochemistry 79, 219–226.CrossRefGoogle ScholarPubMed
Hong, T.D. and Ellis, R.H. (1996) Ex situ biodiversity conservation by seed storage: multiple-criteria keys to estimate seed storage behaviour. Seed Science and Technology 25, 157–161.Google Scholar
ISTA (1993) International rules for seed testing. Rules 1993. Seed Science and Technology 21, Supplement, 1–75.Google Scholar
Musatenko, L.I., Berestetsky, V.A., Vedenicheva, N.P., Generalova, V.N., Martyn, G.I. and Sytnik, K.M. (1995) Phytohormones and structure of cells of Acer saccharinum seed embryo. Biologia Plantarum 37, 553–559.CrossRefGoogle Scholar
Nir, I., Klein, S. and Poljakoff-Mayber, A. (1969) Effect of moisture stress on submicroscopic structure of maize roots. Australian Journal of Biological Sciences 22, 17–33.CrossRefGoogle Scholar
Pammenter, N.W. and Berjak, P. (1999) A review of recalcitrant seed physiology in relation to desiccationtolerance mechanisms. Seed Science Research 9, 13–37.CrossRefGoogle Scholar
Roberts, E.H. (1973) Predicting the storage life of seeds. Seed Science and Technology 1, 499–514.Google Scholar
Sacandé, M., Groot, S.P.C., Hoekstra, F.A., de Castro, R.D. and Bino, R.J. (1997) Cell cycle events in developing neem (Azadirachta indica) seeds: are they related to intermediate storage behaviour? Seed Science Research 7, 161–168.CrossRefGoogle Scholar
Sgorbati, S., Levi, M., Sparvoli, E., Trezzi, F. and Lucchini, G. (1986) Cytometry and flow cytometry of 4',6-diamidino-2-phenylindole (DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiologia Plantarum 68, 471–476.CrossRefGoogle Scholar
Shmatko, I.G. and Kabluchko, O.I. (1980) Effect of water deficit stress on shoot meristem tissues of wheat. Ukrainsky Botanichesky Zhurnal 37, N6, 55–57.Google Scholar
Tetteroo, F.A.A., Bino, R.J., Bergervoet, J.H.W. and Hasenack, B. (1995) Effect of ABA and slow drying on DNA replication in carrot (Daucus carota) embryoids. Physiologia Plantarum 95, 154–158.CrossRefGoogle Scholar
Troyan, V., Kolesnikov, V., Kalinin, F., Zelenin, A. and Ilchenko, L. (1984) The periodicity of the change in the rate of RNA synthesis, endogenous RNA polymerase activity and DNA availability to acridine orange during the first cell cycle in the root meristematic cells of germinating Pisum sativum L. Plant Science Letters 33, 213–219.CrossRefGoogle Scholar
Yanishevsky, R.M. and Stein, G.H. (1981) Regulation of the cell cycle in eukaryotic cells. International Review of Cytology 69, 223–259.CrossRefGoogle ScholarPubMed