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Characterization of microporous membranes using confocal scanning laser microscopy influorescence mode

Published online by Cambridge University Press:  15 December 2000

C. Charcosset  and
J.-C. Bernengo
C. Charcosset*
Affiliation:
Laboratoire d'Automatique et de Génie des Procédés, UCB Lyon 1, UPRES A-Q 5007 CNRS, ESCPE-Lyon, Bât. 308 G, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France
J.-C. Bernengo
Affiliation:
UCB Lyon 1, Centre Commun de Quantimétrie, 8 avenue Rockfeller, 69373 Lyon Cedex 08, France
*
charcosset@lagep.univ-lyon1.fr
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Abstract

Confocal Scanning Laser Microscopy (CSLM) in fluorescence mode was used to characterize microporous membranes. Two microfiltration membranes were investigated: a mixed ester (cellulose nitrate/cellulose acetate) 1.2 μm-rated membrane and a polycarbonate track-etched membrane with cylindrical pores of 2 μm diameter. Optical sections of the membranes stained with rhodamine and mounted in glycerol were performed at 1 μm intervals, from 0 to 10 μm. CSLM was found useful for microporous membrane characterization, as it gives some insight into bulk membrane morphology.

Keywords

Type
Research Article
Information
The European Physical Journal - Applied Physics , Volume 12 , Issue 3 , December 2000 , pp. 195 - 199
Copyright
© EDP Sciences, 2000

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References

L.J. Zeman, A.L. Zydney, Microfiltration and Ultrafiltration, Principles and Applications (Ed: Marcel Dekker, Inc., 1996).
J.B. Pawley, Handbook of Biological Confocal Microscopy (Plenum Press, New York, 1995).
B. Matsumoto, Cell Biological Applications of Confocal Microscopy (Academic Press, Inc., San Diego, 1993).
J.K. Stevens, L.R. Mills, J.E. Trogadis, Three-Dimensional Confocal Microscopy: Volume Investigation of Biological Systems (Cell Biology: A Series of Monographs, Academic Press, Inc., San Diego, 1994).
A.E. Ribbe, TRIP 5, 333 (1997).
A. Ljunglöf, P. Bergvall, R. Bhikhabhai, R. Hjorth, plasmid DNA in individual chromatography adsorbent particles by confocal scanning laser microscopy, J. Chromatogr. A 844, 129 (1999).
C. Charcosset, A. Cherfi, J.-C. Bernengo, Chem. Eng. Sci. (in press).
C. Charcosset, J.-C. Bernengo, J. Membrane Sci. 168, 53 (2000).
A. Longin, C. Souchier, M. Ffrench, P.-A. Bryon, J. Histochem. Cytochem. 41, 1833 (1993).
L. Zeman, J. Membrane Sci. 71, 233 (1992).