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Genetic diversity of biennial wormwood

Published online by Cambridge University Press:  20 January 2017

Lemma W. Mengistu ,
Michael J. Christoffers  and
George O. Kegode
Michael J. Christoffers
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
George O. Kegode
Affiliation:
Department of Plant Sciences, North Dakota State University, Fargo, ND 58105
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Abstract

Biennial wormwood is native to North America and has become an important weed problem in soybean and dry bean fields of North Dakota, South Dakota, and Minnesota in the United States and in the prairie provinces of Canada. Intersimple sequence repeat (ISSR) markers were used to study the genetic diversity among six biennial wormwood and one annual wormwood populations. Deoxyribonucleic acid (DNA) sequences from internal transcribed spacer (ITS1 and ITS2) regions of ribosomal DNA and morphological diversity among the biennial and annual wormwood populations were also studied. High levels of genetic diversity were evident with Nei's gene diversity statistic (h) = 0.40 for biennial wormwood and h = 0.36 for annual wormwood. Total diversity of six biennial wormwood populations was HT = 0.40, and 22% of this diversity was among populations (GST = 0.22). Estimated gene flow among biennial wormwood populations was low (Nm = 0.9), and high levels of differentiation may be due in part to low levels of genetic exchange among biennial wormwood populations. Although biennial wormwood behaves more like an annual than a biennial, the ISSR, ITS, and morphological studies show that the two species are dissimilar.

Keywords

Annual wormwood, Artemisia annua L. ARTANbiennial wormwood, Artemisia biennis Willd. ARTBIdry bean, Phaseolus vulgaris L.soybean, Glycine max L. MerrGenetic diversityintersimple sequence repeat markersinternal transcribed spacers of nuclear ribosomal DNA
Type
Weed Biology and Ecology
Information
Weed Science , Volume 52 , Issue 1 , February 2004 , pp. 53 - 60
Copyright
Copyright © Weed Science Society of America 

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