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Genetics and Inheritance of Nontarget-Site Resistances to Atrazine and Mesotrione in a Waterhemp (Amaranthus tuberculatus) Population from Illinois

Published online by Cambridge University Press:  20 January 2017

Janel Huffman ,
Nicholas E. Hausman ,
Aaron G. Hager ,
Dean E. Riechers  and
Patrick J. Tranel
Janel Huffman
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Nicholas E. Hausman
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Aaron G. Hager
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Dean E. Riechers
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
Patrick J. Tranel*
Affiliation:
Department of Crop Sciences, University of Illinois, Urbana, IL 61801
*
Corresponding author's E-mail: tranel@illinois.edu
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Abstract

A waterhemp population (McLean County resistant, MCR) from McLean County, Illinois is resistant to both mesotrione and atrazine by elevated rates of herbicide metabolism. Research was conducted to investigate the inheritance of these resistance traits. Resistant and sensitive plants were crossed to obtain reciprocal F1 populations, which were then used to create pseudo-F2 and backcross (to sensitive parent; BCS) populations. The various populations were evaluated with whole-plant herbicide efficacy studies in a greenhouse. The responses of the F1 populations to both mesotrione and atrazine were intermediate when compared with parental populations. In the case of atrazine, BCS and F2 populations segregated 1 : 1 and 1 : 3, respectively, for susceptibility (S) : resistance (R), at a dose that controlled the sensitive parent but not the F1 or resistant parent. For mesotrione, variability was observed within the F1 populations, suggesting that mesotrione resistance is multigenic and the resistant parents used in the cross were not homozygous at the resistance loci. Furthermore, at low mesotrione doses, more F2 plants survived than expected on the basis of a single-gene trait, whereas at high doses, fewer F2 plants survived than expected. Dry weight data confirmed the conclusions obtained from survival data. Specifically, atrazine responses segregated into two discrete classes (R and S) in both the F2 and BCS populations, whereas mesotrione responses showed continuous distributions of phenotypes in F2 and BCS populations. We conclude that metabolism-based atrazine resistance in MCR is conferred by a single major gene, whereas inheritance of mesotrione resistance in this population is complex.

Keywords

Atrazinemesotrionecommon waterhemp, Amaranthus tuberculatus (Moq.) Sauer var. rudis (Sauer) Costea and Tardif AMATU.Genetic inheritanceherbicide metabolismHPPDmonogenicmultigenic
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 63 , Issue 4 , December 2015 , pp. 799 - 809
Copyright
Copyright © Weed Science Society of America 

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Footnotes

Associate Editor for this paper: Christopher Preston, University of Adelaide.

References

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