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Reduced Translocation Is Associated with Tolerance of Common Lambsquarters (Chenopodium album) to Glyphosate

Published online by Cambridge University Press:  20 January 2017

Melinda K. Yerka ,
Andrew T. Wiersma ,
R. Bradley Lindenmayer ,
Philip Westra ,
William G. Johnson ,
Natalia de Leon  and
David E. Stoltenberg
Melinda K. Yerka
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
Andrew T. Wiersma
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
R. Bradley Lindenmayer
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
Philip Westra
Affiliation:
Bioagricultural Science and Pest Management, Colorado State University, Fort Collins, CO 80523-1170
William G. Johnson
Affiliation:
Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN 47909
Natalia de Leon
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
David E. Stoltenberg*
Affiliation:
Department of Agronomy, University of Wisconsin-Madison, 1575 Linden Drive, Madison, WI 53705
*
Corresponding author's E-mail: destolte@wisc.edu
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Abstract

Common lambsquarters tolerance to glyphosate is problematic because of the species' widespread distribution, competitive ability with many crop species, the widespread use of glyphosate in agriculture, and the weed's potential to develop decreased sensitivity to multiple herbicide sites of action. The mechanism that confers common lambsquarters tolerance to glyphosate is not known. Therefore, we conducted experiments to determine the mechanism of tolerance to glyphosate in an accession of common lambsquarters from Indiana relative to a sensitive accession from Wisconsin. The ED50 (the effective dose that reduced shoot mass 50% relative to nontreated plants) value for the tolerant accession (1.6 kg ae ha−1 ± 0.4 standard error of the mean [SEM]) was eightfold greater than the ED50 for the sensitive accession (0.2 kg ae ha−1 ± 0.2 SEM) 28 d after treatment. The glyphosate target-site (EPSPS) DNA sequence at proline 106, shikimate accumulation as an estimate of EPSPS sensitivity, and EPSPS protein abundance did not differ between accessions. Absorption of 14C-glyphosate was slightly greater in the tolerant accession than it was in the sensitive accession at 48 and 72 h after treatment (HAT). However, the tolerant accession translocated a smaller percentage of absorbed 14C-glyphosate to the tissue above the treated leaf, which included the shoot apical meristem, at 24, 48, and 72 HAT (P ≤ 0.05, 0.01, and 0.10, respectively). These results suggest an important role of reduced translocation in conferring tolerance of common lambsquarters to glyphosate.

Keywords

EPSPS, 5-enolpyruvylshikimate-3-phosphate synthaseglyphosatecommon lambsquarters, Chenopodium album L. CHEALHerbicidemechanism of actionresistance
Type
Physiology, Chemistry, and Biochemistry
Information
Weed Science , Volume 61 , Issue 3 , September 2013 , pp. 353 - 360
Copyright
Copyright © Weed Science Society of America 

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