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Target site–based penoxsulam resistance in barnyardgrass (Echinochloa crus-galli) from China

Published online by Cambridge University Press:  18 March 2019

Jiapeng Fang ,
Tingting Liu ,
Yuhua Zhang ,
Jun Li  and
Liyao Dong Open the ORCID record for Liyao Dong [Opens in a new window]
Jiapeng Fang
Affiliation:
Ph.D Candidate, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Tingting Liu
Affiliation:
Graduate Student, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Yuhua Zhang
Affiliation:
Ph.D Candidate, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Jun Li
Affiliation:
Associate Professor, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
Liyao Dong*
Affiliation:
Professor, College of Plant Protection, Nanjing Agricultural University, Nanjing, People’s Republic of China; State and Local Joint Engineering Research Center of Green Pesticide Invention and Application, Nanjing, People’s Republic of China
*
Author for correspondence: Liyao Dong, Email: dly@njau.edu.cn
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Abstract

Barnyardgrass [Echinochloa crus-galli (L.) P. Beauv.] is acknowledged to be the most troublesome weed in rice fields in Anhui and Jiangsu provinces of China. It cannot be effectively controlled using certain acetolactate synthase (ALS)-inhibiting herbicides, including penoxsulam. Echinochloa crus-galli samples with suspected resistance to penoxsulam were collected to identify the target site–based mechanism underlying this resistance. Populations AXXZ-2 and JNRG-2 showed 33- and 7.3-fold resistance to penoxsulam, respectively, compared with the susceptible JLGY-3 population. Cross-resistance to other ALS inhibitors was reported in AXXZ-2 but not in JNRG-2, and occasionally showed higher sensitivity than JLGY-3. In vitro ALS activity assays revealed that penoxsulam concentrations required to inhibit 50% of ALS activity were 11 and 5.2 times greater in AXXZ-2 and JNRG-2, respectively, than in JLGY-3. DNA and predicted amino acid sequence analyses of ALS revealed Ala-205-Val and Ala-122-Gly substitutions in AXXZ-2 and JNRG-2, respectively. Our results indicate that these substitutions in ALS are at least partially responsible for resistance to penoxsulam.

Keywords

Patrick J. Tranel, University of IllinoisAcetolactate synthase (ALS)cross-resistancetarget-site mutant
Type
Research Article
Information
Weed Science , Volume 67 , Issue 3 , May 2019 , pp. 281 - 287
Copyright
© Weed Science Society of America, 2019 

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References

Altop, EK, Mennan, H, Streibig, JC, Budak, U, Ritz, C (2014) Detecting ALS and ACCase herbicide tolerant accession of Echinochloa oryzoides (Ard.) Fritsch in rice (Oryza sativa L.) fields. Crop Prot 65:202–206CrossRefGoogle Scholar
Ashigh, J, Tardif, FJ (2017) An Ala205Val substitution in acetohydroxyacid synthase of eastern black nightshade (Solanum ptychanthum) reduces sensitivity to herbicides and feedback inhibition. Weed Sci 55:558–565CrossRefGoogle Scholar
Beckie, HJ, Tardif, FJ (2012) Herbicide cross resistance in weeds. Crop Prot 35:15–28CrossRefGoogle Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254CrossRefGoogle ScholarPubMed
Brosnan, JT, Vargas, JJ, Breeden, GK, Grier, L, Aponte, RA, Tresch, S, Laforest, M (2016) A new amino acid substitution (Ala-205-Phe) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS-inhibiting herbicides. Planta 243:149–159CrossRefGoogle ScholarPubMed
Chen, GQ, Wang, Q, Yao, ZW, Zhu, LF, Dong, LY (2016) Penoxsulam-resistant barnyardgrass (Echinochloa crus-galli) in rice fields in China. Weed Biol Manag 16:16–23CrossRefGoogle Scholar
Duggleby, RG, McCourt, JA, Guddat, LW (2008) Structure and mechanism of inhibition of plant acetohydroxyacid synthase. Plant Physiol Biochem 46:309–324CrossRefGoogle ScholarPubMed
Duggleby, RG, Pang, SS, Yu, HQ, Guddat, LW (2003) Systematic characterization of mutations in yeast acetohydroxyacid synthase—interpretation of herbicide-resistance data. Eur J Biochem 270:2895–2904CrossRefGoogle ScholarPubMed
Feng, Y, Gao, Y, Zhang, Y, Dong, L, Li, J (2017) Mechanisms of resistance to pyroxsulam and ACCase inhibitors in Japanese foxtail (Alopecurus japonicus). Weed Sci 64:695–704CrossRefGoogle Scholar
Han, H, Yu, Q, Purba, E, Li, M, Walsh, M, Friesen, S, Powles, SB (2012) A novel amino acid substitution Ala-122-Tyr in ALS confers high-level and broad resistance across ALS-inhibiting herbicides. Pest Manag Sci 68:1164–1170CrossRefGoogle ScholarPubMed
Iwakami, S, Hashimoto, M, Matsushima, K, Watanabe, H, Hamamura, K, Uchino, A (2015) Multiple-herbicide resistance in Echinochloa crus-galli var. formosensis, an allohexaploid weed species, in dry-seeded rice. Pestic Biochem Physiol 119:1–8CrossRefGoogle ScholarPubMed
Iwakami, S, Shimono, Y, Manabe, Y, Endo, M, Shibaike, H, Uchino, A, Tominaga, T (2017) Copy number variation in acetolactate synthase genes of thifensulfuron-methyl resistant Alopecurus aequalis (shortawn foxtail) accessions in Japan. Front Plant Sci 8:254CrossRefGoogle Scholar
Iwakami, S, Uchino, A, Watanabe, H, Yamasue, Y, Inamura, T (2012) Isolation and expression of genes for acetolactate synthase and acetyl-CoA carboxylase in Echinochloa phyllopogon, a polyploid weed species. Pest Manag Sci 68:1098–1106CrossRefGoogle ScholarPubMed
Kaloumenos, NS, Chatzilazaridou, SL, Mylona, PV, Polidoros, AN, Eleftherohorinos, IG (2013) Target-site mutation associated with cross-resistance to ALS-inhibiting herbicides in late watergrass (Echinochloa oryzicola Vasing.). Pest Manag Sci 69:865–873CrossRefGoogle Scholar
Krysiak, M, Gawroński, S, Adamczewski, K, Kierzek, R (2011) ALS gene mutations in Apera spica-venti confer broad-range resistance to herbicides. J Plant Prot Res 51:261–267CrossRefGoogle Scholar
Le, DT, Yoon, MY, Kim, YT, Choi, JD (2005) Two consecutive aspartic acid residues conferring herbicide resistance in tobacco acetohydroxy acid synthase. Biochim Biophys Acta 1749:103–112CrossRefGoogle ScholarPubMed
Matzenbacher, FO, Bortoly, ED, Kalsing, A, Merotto, A (2015) Distribution and analysis of the mechanisms of resistance of barnyardgrass (Echinochloa crus-galli) to imidazolinone and quinclorac herbicides. J Agric Sci 153:1044–1058CrossRefGoogle Scholar
Matzrafi, M, Lazar, TW, Sibony, M, Rubin, B (2015) Conyza species: distribution and evolution of multiple target-site herbicide resistances. Planta 242:259–267CrossRefGoogle ScholarPubMed
McNaughton, KE, Letarte, J, Lee, EA, Tardif, FJ (2005) Mutations in ALS confer herbicide resistance in redroot pigweed (Amaranthus retroflexus) and Powell amaranth (Amaranthus powellii). Weed Sci 53:17–22CrossRefGoogle Scholar
Ni, H, Moody, K, Robles, RP, Paller, EC Jr, Lales, JS, Cosico, WC (1996) Effect of nitrogen rate on competition of two rice cultivars against Echinochloa crus-galli. Philippine J Weed Sci 24:53–62Google Scholar
Norsworthy, JK, Wilson, MJ, Scott, RC, Gbur, EE (2014) Herbicidal activity on acetolactate synthase-resistant barnyardgrass (Echinochloa crus-galli) in Arkansas, USA. Weed Biol Manag 14:50–58CrossRefGoogle Scholar
Panozzo, S, Scarabel, L, Rosan, V, Sattin, M (2017) A new Ala-122-Asn amino acid change confers decreased fitness to ALS-resistant Echinochloa crus-galli. Front Plant Sci 8:13CrossRefGoogle ScholarPubMed
Panozzo, S, Scarabel, L, Tranel, PJ, Sattin, M (2013) Target-site resistance to ALS inhibitors in the polyploid species Echinochloa crus-galli. Pestic Biochem Physiol 105:93–101CrossRefGoogle Scholar
Powles, SB, Yu, Q (2010) Evolution in action: plants resistant to herbicides. Annu Rev Plant Biol 61:317–347CrossRefGoogle ScholarPubMed
Ray, TB (1984) Site of action of chlorsulfuron: inhibition of valine and isoleucine biosynthesis in plants. Plant Physiol 75:827–831CrossRefGoogle ScholarPubMed
Riar, DS, Norsworthy, JK, Srivastava, V, Nandula, V, Bond, JA, Scott, RC (2013) Physiological and molecular basis of acetolactate synthase-inhibiting herbicide resistance in barnyardgrass (Echinochloa crus-galli). J Agric Food Chem 61:278–289CrossRefGoogle Scholar
Song, JS, Lim, SH, Yook, MJ, Kim, JW, Kim, DS (2017) Cross-resistance of Echinochloa species to acetolactate synthase inhibitor herbicides. Weed Biol Manag 17:91–102CrossRefGoogle Scholar
Tranel, PJ, Wright, TR, Heap, IM (2018) Mutations in herbicide-resistant weeds to ALS inhibitors. www.weedscience.com. Accessed: March 12, 2018Google Scholar
Xu, HL, Li, J, Zhang, D, Cheng, Y, Jiang, Y, Dong, LY (2014) Mutations at codon position 1999 of acetyl-CoA carboxylase confer resistance to ACCase-inhibiting herbicides in Japanese foxtail (Alopecurus japonicus). Pest Manag Sci 70:1894–1901CrossRefGoogle Scholar
Yu, Q, Ahmad-Hamdani, MS, Han, H, Christoffers, MJ, Powles, SB (2013) Herbicide resistance-endowing ACCase gene mutations in hexaploid wild oat (Avena fatua): insights into resistance evolution in a hexaploid species. Heredity 110:220–231CrossRefGoogle Scholar
Yu, Q, Friesen, LJS, Zhang, XQ, Powles, SB (2004) Tolerance to acetolactate synthase and acetyl-coenzyme A carboxylase inhibiting herbicides in Vulpia bromoides is conferred by two co-existing resistance mechanisms. Pestic Biochem Physiol 78:21–30CrossRefGoogle Scholar
Yu, Q, Powles, SB (2014) Resistance to AHAS inhibitor herbicides: current understanding. Pest Manag Sci 70:1340–1350CrossRefGoogle ScholarPubMed
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