Hostname: page-component-78c5997874-g7gxr Total loading time: 0 Render date: 2024-11-17T18:08:45.214Z Has data issue: false hasContentIssue false
  • English
  • Français

A Diagnostic Assay to Detect Herbicide Resistance in Annual Bluegrass (Poa annua)

Published online by Cambridge University Press:  21 July 2017

James T. Brosnan ,
Jose J. Vargas ,
Eric H. Reasor ,
Roberto Viggiani ,
Gregory K. Breeden  and
John M. Zobel
James T. Brosnan*
Affiliation:
Associate Professor, Research Associate, Graduate Research Assistant, Visiting Scholar, and Extension Specialist, Department of Plant Sciences, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
Jose J. Vargas
Affiliation:
Associate Professor, Research Associate, Graduate Research Assistant, Visiting Scholar, and Extension Specialist, Department of Plant Sciences, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
Eric H. Reasor
Affiliation:
Associate Professor, Research Associate, Graduate Research Assistant, Visiting Scholar, and Extension Specialist, Department of Plant Sciences, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
Roberto Viggiani
Affiliation:
Associate Professor, Research Associate, Graduate Research Assistant, Visiting Scholar, and Extension Specialist, Department of Plant Sciences, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
Gregory K. Breeden
Affiliation:
Associate Professor, Research Associate, Graduate Research Assistant, Visiting Scholar, and Extension Specialist, Department of Plant Sciences, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
John M. Zobel
Affiliation:
Assistant Professor, Department of Forestry, Wildlife, and Fisheries, University of Tennessee Institute of Agriculture, 2431 Joe Johnson Drive, Knoxville, TN 37996
*
*Corresponding author’s E-mail: jbrosnan@utk.edu
Get access Rights & Permissions [Opens in a new window]

Abstract

Turfgrass managers currently have few readily available means of evaluating herbicide resistance in annual bluegrass during the growing season. Research was conducted to determine if agar-based diagnostic tests developed for agronomic weeds could be used to reliably confirm herbicide resistance in annual bluegrass harvested from golf course turf. Annual bluegrass phenotypes with target-site resistance to acetolactate synthase (ALS; R3, R7), enolpyruvylshikimate-3-phosphate synthase (EPSPS; R5), and photosystem II (PSII; R3, R4) inhibiting herbicides were included in experiments along with an herbicidal susceptible phenotype (S). Single tiller plants were washed free of soil and transplanted into autoclavable polycarbonate plant culture boxes filled with plant tissue culture agar amended with a murashigee-skoog medium and trifloxysulfuron (6.25, 12.5, 25, 50, 75, 100, or 150 μM), glyphosate (0, 6, 12, 25, 50, 100, 200, or 400 μM), or simazine (0, 6, 12, 25, 50, 100, 200, or 400 μM). Mortality in agar was assessed 7 to 10 days after treatment (depending on herbicide) and compared to responses observed after treating individual plants of each phenotype with trifloxysulfuron (28 g ai ha-1), glyphosate (1120 g ae ha-1), or simazine (1120 g ai ha-1) in an enclosed spray chamber. Fisher’s exact test (α = 0.05) determined that mortality in agar with 12.5 μM trifloxysulfuron and 100 μM glyphosate was not significantly different than treating whole plants via traditional spray application. Mortality with all concentrations of simazine in agar was significantly different than that observed after treating resistant and susceptible phenotypes via traditional spray application. Our findings indicate that an agar-based diagnostic assay can be used to detect annual bluegrass resistance to ALS- or EPSPS-inhibiting herbicides in less than 10 days; however, additional research is needed to refine this assay for use with PSII-inhibiting herbicides.

Keywords

Glyphosatesimazinetrifloxysulfuronannual bluegrass, Poa annua L.Diagnosticsgolf courseresistance managementsod productionturfturfgrass.
Type
Weed Management-Other Crops/Areas
Information
Weed Technology , Volume 31 , Issue 4 , August 2017 , pp. 609 - 616
Copyright
© Weed Science Society of America, 2017 

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Footnotes

Associate Editor for this paper: Scott McElroy, Auburn University.

References

Literature Cited

Anonymous (2014) Princep Liquid product label. Greensboro, NC: Syngenta Crop Protection Google Scholar
Anonymous (2016) Xonerate product label. Greensboro, NC: Arysta LifeSciences Google Scholar
Brosnan, JT, Vargas, JJ, Breeden, GK, Boggess, SL, Staton, MA, Wadl, PA, Trigiano, RN (2017) Controlling herbicide resistant annual bluegrass (Poa annua L.) with methiozolin. Weed Technol 31:470–476 CrossRefGoogle Scholar
Brosnan, JT, Vargas, JJ, Breeden, GK, Grier, L, Aponte, RA, Tresch, S, LaForest, M (2016) A new amino acid substitution (Ala-205-Phe) in acetolactate synthase (ALS) confers broad spectrum resistance to ALS-inhibiting herbicides. Planta 243:149–159 CrossRefGoogle ScholarPubMed
Burgos, NR, Tranel, PJ, Streibig, JC, Davis, VM, Shaner, D, Norsworthy, JK, Ritz, C (2013) Confirmation of resistance to herbicides and evaluation of resistance levels. Weed Sci 61:4–20 CrossRefGoogle Scholar
Cross, RB, McCarty, LB, Tharayil, N, Whitwell, T, Bridges, WC (2013) Detecting annual bluegrass (Poa annua) resistance to ALS-inhibiting herbicides using a rapid diagnostic assay. Weed Sci 61:384–389 CrossRefGoogle Scholar
Dayan, FE, Trindade, MLB, Velini, EV (2009) Amicarbazone: a new photosystem II inhibitor. Weed Sci 57:579–583 CrossRefGoogle Scholar
Dèlye, C, Duhoux, A, Pernin, F, Riggins, CW, Tranel, PJ (2015) Molecular mechanisms of herbicide resistance. Weed Sci (Spec. Issue): 91–115 CrossRefGoogle Scholar
Duhoux, A, Carrère, S, Gouzy, J, Bonin, L, Dèlye, C (2015) RNA-Seq analysis of rye-grass transcriptomic response to an herbicide inhibiting acetolactate synthase identifies transcripts linked to non-target site based resistance. Plant Mol Biol 87:473–487 CrossRefGoogle Scholar
Heap, I (2016) International Survey of Herbicide Resistant Weeds. http://www.weedscience.org. Accessed March 9, 2017Google Scholar
Jackson, L, Hutchings, SJ, Harris, S, Dale, R, Mcindoe, E, Kaundun, SS (2015) The Syngenta RISQ test: a universal early season method for detecting weed resistance to herbicides. Page 57 in Proceedings of Resistance 15. Hertfordshire, UK: Rothamsted Research, HarpendenGoogle Scholar
Kaundun, SS, Hutchings, SJ, Dale, RP, Baily, GC, Glanfield, P (2011) Syngenta ‘RISQ’ test: a novel in-season method for detecting resistance to postemergence ACCase and ALS inhibitor herbicides in grass weeds. Weed Res 51:284–293 CrossRefGoogle Scholar
Kaundun, SS, Hutchings, SJ, Harris, SC, Jackson, LV, Shashi-Kiran, R, Dale, RP, McIndoe, E (2014) A simple in-season bioassay for detecting glyphosate resistance in grass and broadleaf weeds prior to herbicide application in the field. Weed Sci 62:597–607 CrossRefGoogle Scholar
Perry, DH, McElroy, JS, Dane, F, van Santen, E, Walker, RH (2012) Triazine-resistant annual bluegrass (Poa annua) populations with Ser264 mutation are resistant to amicarbazone. Weed Sci 60:355–359 CrossRefGoogle Scholar
Svyantek, AW, Aldahir, P, Chen, S, Flessner, ML, McCullough, PE, Sidhu, SS, McElroy, JS (2016) Target and nontarget resistance mechanisms induce annual bluegrass (Poa annua) resistance to atrazine, amicarbazone, and diuron. Weed Technol 30:773–782 CrossRefGoogle Scholar