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Syngenta Quick-Test: A Rapid Whole-Plant Test for Herbicide Resistance

Published online by Cambridge University Press:  20 January 2017

Peter Boutsalis
Peter Boutsalis*
Affiliation:
Syngenta Crop Protection, WST-149, Stein 4332, Switzerland
*
E-mail: peter.boutsalis@syngenta.com.
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Abstract

A diagnostic test (Syngenta Quick-Test, QT) used for testing grass weed survivors to herbicides in the field for resistance was evaluated. Cuttings from grass weeds were transplanted into pots to regenerate new leaves, then treated with herbicide. In greenhouse experiments, resistance of known herbicide-resistant blackgrass biotypes to the aryloxyphenoxypropanoate herbicides CGA 184927 and fenoxaprop-ethyl and to the phenylurea herbicide isoproturon was verified by the QT. The findings were similar to those for seedlings grown from seed. Rigid ryegrass from suspect resistant fields in South Australia was sampled and sent by post to Switzerland for QT analysis. Resistance was confirmed in less than 4 wk, which verified resistance as responsible for the field failures. The added features of the QT over current resistance tests suggest a likely fit for in-season testing of surviving weeds and possible follow-up action.

Keywords

CGA 184927, (propynyl (R)-2-[4-(5-chloro-3-fluoro-2-pyridyloxy)phenoxy]-propanoate)fenoxaprop-ethylisoproturonblackgrass, Alopecurus myosuroides Huds. #3 ALOMYrigid ryegrass, Lolium rigidum Gaudin # LOLRIResistance testaryloxyphenoxypropanoatephenylureaACCase, acetyl coenzyme A carboxylase (EC 6.4.1.2)APP, aryloxyphenoxypropanoateCHD, cyclohexanedioneDAT, days after treatmentPU, phenylureaQT, Syngenta Quick-TestSU, sulfonylurea
Type
Research
Information
Weed Technology , Volume 15 , Issue 2 , June 2001 , pp. 257 - 263
Copyright
Copyright © Weed Science Society of America 

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References

Literature Cited

Cocker, K. M., Moss, S. R., and Coleman, J.O.D. 1999. Multiple mechanisms of resistance to fenoxaprop-P-ethyl in United Kingdom and other European populations of herbicide-resistant Alopecurus myosuroides (Black-Grass). Pestic. Biochem. Physiol. 65: 169–180.CrossRefGoogle Scholar
De Prado, R., Menendez, J., Tena, M., Caseley, J., and Tabener, A. 1991. Response to substituted ureas, triazines and chloroacetanilides in a biotype of Alopecurus myosuroides resistant to chlorotoluron. In Proc. Brighton Crop Prot. Conf.—Weeds. pp. 1065–1070.Google Scholar
Devine, M. D. and Shimabukuro, R. H. 1994. Resistance to acetyl coenzyme A carboxylase inhibiting herbicides. In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers. pp. 141–170.Google Scholar
Gerwick, B. C., Mireles, L. C., and Eilers, R. J. 1993. Rapid diagnosis of ALS/AHAS-resistant weeds. Weed Technol. 7: 519–524.CrossRefGoogle Scholar
Gill, G. S. 1990. Evaluation of the Petri-dish assay for screening diclofop-methyl resistance in partially resistant populations of annual ryegrass, Lolium rigidum. In Proc. 9th Aust. Weeds Conf., Adelaide, South Australia. pp. 220–223.Google Scholar
Hall, L. M., Holtum, J.A.M., and Powles, S. B. 1994. Mechanisms responsible for cross resistance and multiple resistance. In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers. pp. 243–262.Google Scholar
Harker, K. N. and Dekker, J. 1988. Effects of phenology on translocation patterns of several herbicides in quackgrass, Agropyron repens . Weed Sci. 36: 463–472.CrossRefGoogle Scholar
Heap, I. 1999. International Survey of Herbicide Resistant Weeds. Online. Internet. January 6th, 2000. Available at www.weedscience.com.Google Scholar
Heap, I. and Knight, R. 1986. The occurrence of herbicide cross-resistance in a population of annual ryegrass, Lolium rigidum, resistant to diclofop-methyl. Aust. J. Agric. Res. 37: 149–156.Google Scholar
Hensley, J. R. 1981. A method for identification of triazine resistant and susceptible biotypes of several weeds. Weed Sci. 29: 70–73.CrossRefGoogle Scholar
Holm, L. G., Plucknett, D. L., Pancho, J. V., and Herberger, J. P. 1977. The World's Worst Weeds. Distribution and Biology. Honolulu: University Press of Hawaii. pp. 107–110.Google Scholar
James, E. H., Kemp, M. S., and Moss, S. R. 1995. Phytotoxicity of trifluoromethyl- and methyl-substituted dinitroaniline herbicides on resistant and susceptible populations of black-grass (Alopecurus myosuroides). Pestic. Sci. 43: 273–277.CrossRefGoogle Scholar
Letouzé, A., Gasquez, J., Vaccara, D., Orlando, D., Leterrier, J. L., Roy, C., and Bouvard-Derieux, E. 1997. Development of new reliable quick tests and state of grass-weed herbicide resistance in France. In Proc. Brighton Crop Prot. Conf.—Weeds. pp. 325–330.Google Scholar
Maneechote, C., Holtum, J.A.M., Preston, C., and Powles, S. B. 1994. Resistant acetyl-CoA carboxylase is a mechanism of herbicide resistance in a biotype of Avena sterilis ssp. ludoviciana . Plant Cell Physiol. 35: 627–635.Google Scholar
Maneechote, C., Preston, C., and Powles, S. B. 1997. A diclofop-methyl resistant Avena sterilis biotype with a herbicide-resistant acetyl-coenzyme A carboxylase and enhanced metabolism of diclofop-methyl. Pestic. Sci. 49: 105–114.3.0.CO;2-3>CrossRefGoogle Scholar
Matthews, J. M., Holtum, J.A.M., Liljegren, D. R., Furness, B., and Powles, S. B. 1990. Cross-resistance to herbicides in annual ryegrass (Lolium rigidum). I. Properties of the herbicide target enzymes acetyl-coenzyme A carboxylase and acetolactate synthase. Plant Physiol. 94: 1180–1186.Google ScholarPubMed
Maxwell, B. D. and Mortimer, A. M. 1994. Selection for herbicide resistance. In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers. pp. 1–26.Google Scholar
McAlister, F. M., Holtum, J.A.M., and Powles, S. B. 1995. Dinitroaniline herbicide resistance in rigid ryegrass (Lolium rigidum). Weed Sci. 43: 55–62.Google Scholar
Menendez, J. and De Prado, R. 1996. Diclofop-methyl cross-resistance in a chlortoluron-resistant biotype of Alopecurus myosuroides . Pestic. Biochem. Physiol. 56: 123–133.CrossRefGoogle Scholar
Menendez, J. and De Prado, R. 1997. Detoxification of chlortoluron in a chlorotoluron-resistant biotype of Alopecurus myosuroides . Comparison between cell cultures and whole plants. Physiol. Plant. 99: 97–104.Google Scholar
Monaghan, N. M. 1980. The biology and control of Lolium rigidum as a weed of wheat. Weed Res. 20: 117–121.Google Scholar
Moss, S. R. and Clarke, J. H. 1995. Inheritance of herbicide resistance in Black-grass (Alopecurus myosuroides) and responses of the weed to a range of herbicides. In Home-Grown Cereals Authority Project Report 116. p. 71.Google Scholar
Moss, S. R. 2000. The Rothamsted rapid resistance test for detecting herbicide-resistance in annual grass-weeds. Weed Sci. Soc. Am. 102:40.Google Scholar
Murray, B. G., Friesen, L. F., Beaulieu, K. J., and Morrison, I. N. 1996. A seed bioassay to identify acetyl-CoA carboxylase inhibitor resistant wild oat (Avena fatua) populations. Weed Technol. 10: 85–89.Google Scholar
O'Donovan, J. T., Rashid, A., Van Nguyen, H., Newman, J. C., Khan, A., Johnson, C. I., Blackshaw, R. E., and Harker, K. N. 1996. A seedling bioassay for assessing the response of wild oat (Avena fatua) populations to triallate. Weed Technol. 10: 931–935.Google Scholar
Preston, C., Tardif, F., Christopher, J. T., and Powles, S. B. 1996. Multiple resistance to dissimilar herbicide chemistries in a biotype of Lolium rigidum due to enhanced activity of several herbicide degrading enzymes. Pestic. Biochem. Physiol. 54: 2, 123-134.CrossRefGoogle Scholar
Price, L. J., Harwood, J. L., Cole, D. J., and Moss, S. R. 1997. Characteristics of acetyl-CoA carboxylases from graminicide-tolerant grasses. In Proc. Brighton Crop Prot. Conf.—Weeds. pp. 783–788.Google Scholar
Richter, J. and Powles, S. B. 1993. Pollen expression of herbicide target site resistance genes in annual ryegrass (Lolium rigidum). Plant Physiol. 102: 1037–1041.CrossRefGoogle ScholarPubMed
Saari, L. L., Cotterman, J. C., and Thill, D. C. 1994. Resistance to acetolactate synthase inhibiting herbicides. In Powles, S. B. and Holtum, J.A.M., eds. Herbicide Resistance in Plants: Biology and Biochemistry. Boca Raton, FL: Lewis Publishers. pp. 83–140.Google Scholar
Smeda, R. J., Barrentine, W. L., Snipes, C. E., and Rippee, J. H. 1995. Assays to identify graminicide resistance in johnsongrass (Sorghum halepense (L.) Pers.). In Proc. South Weed Sci. Soc., Stoneville, MS. pp. 170–171.Google Scholar
Smeda, R. J., Vaughn, K. C., and Morrison, I. N. 1992. A novel pattern of herbicide cross-resistance in a trifluralin-resistant biotype of green foxtail (Setaria viridis (L.) Beauv.). Pestic. Biochem. Physiol. 42: 227–241.Google Scholar
Tardif, F. J., Holtum, J.A.M., and Powles, S. B. 1993. Occurrence of a herbicide-resistant acetyl-coenzyme A carboxylase mutant in annual ryegrass (Lolium rigidum) selected by sethoxydim. Planta. 190: 176–181.Google Scholar
Tardif, F. J., Preston, C., Holtum, J.A.M., and Powles, S. B. 1996. Resistance to acetyl-coenzyme A carboxylase-inhibiting herbicides endowed by a single major gene encoding a resistant target site in a biotype of Lolium rigidum . Aust. J. Plant Physiol. 23: 15–23.Google Scholar
Thurston, J. M. 1961. The effect of depth of burying and frequency of cultivation on survival and germination of seeds of wild oats (Avena fatua L. and Avena ludoviciana Dur.). Weed Res. 1: 19–31.CrossRefGoogle Scholar
Van Oorschot, J.L.P. and Van Leeuwen, P. H. 1992. Use of fluorescence induction to diagnose resistance of Alopecurus myosuroides Huds. (blackgrass) to chlortoluron. Weed Res. 32: 473–482.CrossRefGoogle Scholar
Walsh, M. J., Duane, R. D., and Powles, S. B. 2001. High frequency of chlorsulfuron resistant wild radish (Raphanus raphanistrum L) populations across the Western Australian wheatbelt. Weed Technol. In press.Google Scholar
Wellington, P. S. and Hitchings, S. 1965. Germinations and seedling establishment of blackgrass (Alopecurus myosuroides Huds.). J. Natl. Inst. Agric. Bot. 10: 262–273.Google Scholar
Wellington, P. S. and Hitchings, S. 1966. Seed dormancy and the winter annual habit in blackgrass (Alopecurus myosuroides Huds.). J. Natl. Inst. Agric. Bot. 10: 628–643.Google Scholar
Yates, F. 1934. Contingency tables involving small numbers and the chi squared test. J. R. Stat. Soc. Suppl. I:217–235.CrossRefGoogle Scholar