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A Hypothesis of Amitrole Action Based on its Behavior Toward Free Radical Generating Systems

Published online by Cambridge University Press:  12 June 2017

Paul Castelfranco  and
Milford S. Brown
Paul Castelfranco
Affiliation:
University of California, Davis, California
Milford S. Brown
Affiliation:
University of California, Davis, California
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Abstract

This paper extends previous observations on the interaction of 3-amino-1,2,4-triazole (amitrole) and riboflavin leading to the decomposition of amitrole to include the protective effect of amitrole on the photodestruction of flavins and flavoproteins. Photoactivation of radioactive amitrole in the presence of riboflavin causes the labeling of inert protein. Cupric ion, ascorbic acid, and oxygen can replace riboflavin and light in the destruction of amitrole and in the labeling of inert protein. The inhibition of fatty acid peroxidase previously reported by Castelfranco has been shown to be irreversible and to require the presence of a hydrogen peroxide generating system. The authors suggest a hypothesis for the mode of action of amitrole which depends upon the ability of this compound to interact with free radical generating systems, presumably by undergoing a one-electron oxidation.

Type
Research Article
Information
Weeds , Volume 11 , Issue 2 , April 1963 , pp. 116 - 124
Copyright
Copyright © 1963 Weed Science Society of America 

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References

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