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From biogas energy, biotechnology to new agriculture

Published online by Cambridge University Press:  31 July 2012

J.C.H. SHIH*
Affiliation:
North Carolina State University, Raleigh, North Carolina, USA / BioResource International, Inc., Morrisville, North Carolina, USA / Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan, ROC
*
Corresponding author: jasonshih.bri@gmail.com
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Abstract

Agriculture in the 21st Century is facing new challenges and transformation according to five general trends: commercialisation, internationalisation, science and technology, environment and energy production. In this laboratory, a series of research, development and commercialisation of biogas and enzyme technologies reflect these trends. Anaerobic digestion is a microbial process that converts organic waste into biogas, containing 60-70% methane. A simple thermophilic anaerobic digestion (TAnD) system for poultry manure was developed from the lab to the farm. TAnD at 50 to 60°C produces biogas at high rates and, as a result, the process is efficient and the digester is a compact unit. A pilot plug-flow TAnD was first constructed and operated at NCSU research farm. Multiple benefits of the system were demonstrated. Supported by UNDP, a TAnD to process five tons of manure daily (from 50,000 hens) was constructed in China and has been operating for 20 years. Large scale digesters processing hundreds of tons of manure daily are increasingly popular in China and Europe. Biogas from large digesters can be a significant source of energy to generate electricity or to fuel transportation. Beyond energy production, the digester is a rich source of biomaterials. A feather-degrading bacterium was discovered and isolated from TAnD, and its keratinase enzyme and the gene encoding for the enzyme were subsequently isolated. Genetic manipulation for over-expression and up-scale fermentation have now made the industrial production of the enzyme possible. As a heat-stable protease, the keratinase was found to be useful in processing feather meal, in enhancing feed digestibility and was able to degrade prion proteins (implicated as the disease vector in bovine spongiform encephalopathy).

Type
Reviews
Copyright
Copyright © World's Poultry Science Association 2012

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References

BRENDELLI, A., DAROIT, D.J. and REFFEL, A. (2009) Biochemical features of microbial keratinases and their production and applications. Applied Microbiology and Biotechnology, DOI 10.1007/s00253-009-2395-5.Google Scholar
GUPTA, R. and RAMNANI, P. (2006) Microbial keratinases and their prospective applications: an overview. Applied Microbiology and Biotechnology 70:21-23.CrossRefGoogle ScholarPubMed
HUANG, J.J.H. and SHIH, J.C.H. (1981) The Potential of biological methane generation from chicken manure. Biotechnology and Bioengineering 23: 2307-2314.CrossRefGoogle Scholar
LANGEVELD, J.P.M., WANG, J.J., SHIH, G.C., BOSSERS, A., GARSSEN, J. and SHIH, J.C.H. (2003) Enzymatic inactivation of prion protein in brain stem from infected cattle and sheep. Journal of Infectious Diseases 188: 1782-1789.CrossRefGoogle ScholarPubMed
LIN, X., LEE, C.G., CASALE, E.S. and SHIH, J.C.H. (1992) Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis. Applied and Environmental Microbiology 85: 3271-3275.CrossRefGoogle Scholar
LIN, X., KELEMEN, D.W., MILLER, E.S. and SHIH, J.C.H. (1995) Nucleotide sequence and expression of kerA, the gene encoding a keratinolytic protease of Bacillus licheniformis PWD- 1. Applied Environmental Microbiology 61: 1469-1474.CrossRefGoogle ScholarPubMed
ODETALLAH, N.H., WANG, J.J., GARLICH, J.D. and SHIH, J.C.H. (2003) Effect of keratinase on growth performance of broiler chicks fed starter diets. Poultry Science 82: 664-670.CrossRefGoogle Scholar
ODETALLAH, N., WANG, J.J., GARLICH, J.D. and SHIH, J.C.H. (2005) Versazyme supplementation of broiler diets improves market growth performance. Poultry Science 84: 858-864.CrossRefGoogle ScholarPubMed
PRUSINER, S.B. (1991) Molecular biology of prion diseases. Science 252: 1515-1522.CrossRefGoogle ScholarPubMed
SHIH, J.C.H. (1985) Holistic farming: an energy, ecology, and health integrated poultry farm, p. 581-590, in Anaerobic Digestion 1985, China State Biogas Association, China.Google Scholar
SHIH, J.C.H. (1987) From anaerobic digestion to holistic farming, in: WISE, D.L. (Ed.) Bioenvironmental System, Vol. II, pp. 1-16 (CRC Press, Boca Raton, Florida).Google Scholar
SHIH, J.C.H. (1993) Recent development in poultry waste digestion and feather utilization, Poultry Science 72: 1617-1620.CrossRefGoogle Scholar
SHIH, J.C.H. and WANG, J.J. (2006) Keratinase technology: from feather degradation and feed additive, to prion destruction. CAB Reviews: Perspectives of Agriculture, Veterinarian Science, Nutrition, and Natural Resources. 1, No. 42, 1-6. Available online: http://www.cababstractsplus.org/cabreviewsCrossRefGoogle Scholar
STEINSBERGER, S.C. and SHIH, J.C.H. (1984) The construction and operation of a low-cost poultry waste digester. Biotechnology and Bioengineering 26: 537-543.CrossRefGoogle ScholarPubMed
WANG, J.J. and SHIH, J.C.H. (1999) Fermentation production of keratinase from Bacillus licheniformis PWD-1 and recombinant B. subtilis FDB-29. Journal of Industrial Microbiology and Biotechnology 22: 608-616.CrossRefGoogle ScholarPubMed
WANG, J.J., GARLICH, J.D. and SHIH, J.C.H. (2006) Versazyme, a keratinase feed additive, improves body weight, feed conversion and breast yield of broiler chickens. Journal of Applied Poultry Research 15: 544-550.CrossRefGoogle Scholar
WILLIAMS, C.M., RICHTER, C.S., MACKENZIE, J.M. JR. and SHIH, J.C.H. (1990) Isolation, identification and characterization of a feather-degrading bacterium. Applied Environmental Microbiology 56: 1509-1515.CrossRefGoogle ScholarPubMed