Hostname: page-component-7bb8b95d7b-2h6rp Total loading time: 0 Render date: 2024-09-05T09:17:34.918Z Has data issue: false hasContentIssue false
  • English
  • Français

Morphokinetics and in vitro developmental potential of monopronucleated ICSI zygotes until the blastocyst stage

Published online by Cambridge University Press:  11 March 2020

Silvia Mateo Open the ORCID record for Silvia Mateo [Opens in a new window] ,
Francesca Vidal ,
Beatriz Carrasco ,
Ignacio Rodríguez ,
Buenaventura Coroleu ,
Anna Veiga  and
Montserrat Boada
Silvia Mateo*
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain
Francesca Vidal
Affiliation:
Cell Biology Unit, Faculty of Biosciences, Universitat Autònoma de Barcelona, Bellatera, Spain
Beatriz Carrasco
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain
Ignacio Rodríguez
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain
Buenaventura Coroleu
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain
Anna Veiga
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain Barcelona Stem Cell Bank, Centre of Regenerative Medicine in Barcelona, Hospital Duran i Reynals, Gran Via de l’Hospitalet 199, 08908 Hospitalet de Llobregat, Barcelona, Spain
Montserrat Boada
Affiliation:
Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Barcelona, Spain
*
Author for correspondence: Silvia Mateo. Reproductive Medicine Service, Department of Obstetrics, Gynaecology and Reproduction, Women’s Health Dexeus, Gran Via Carles III, 71–75, 08028, Barcelona, Spain. Tel: +34 93 2274700. E-mail: silmat@dexeus.com
Get access Rights & Permissions [Opens in a new window]

Summary

The aim of this study was to provide a more comprehensive understanding of 1PN intracytoplasmic sperm injection (ICSI) zygotes. To achieve this objective, we assessed whether all 1PN-derived embryos showed a similar morphokinetic pattern, and if the morphokinetic behaviour of 1PN-derived embryos was comparable with that of 2PN-derived embryos. In total, 149 1PN ICSI zygotes (study group) and 195 2PN ICSI zygotes (control group) were included in the study. Embryo development potential was evaluated in terms of blastocyst rate. Morphokinetic parameters, including the pronucleus diameter and kinetics of in vitro development, were also analyzed. Embryos derived from 1PN ICSI zygotes showed impaired development compared with 2PN-derived embryos, with blastocyst rates of 28.9% and 67.2%, respectively. The diameter of the pronucleus of 1PN zygotes was larger than that of 2PN zygotes. When compared with 2PN-derived embryos, those derived from 1PN zygotes had a visible pronucleus for a shorter time, in addition to a longer syngamy time and slower kinetic behaviour from two to nine cells. When 1PN-derived blastocysts and 2PN-derived blastocysts were compared, the developmental kinetics were similar in both groups, except for a delayed and longer duration of the compaction phase in 1PN-derived embryos. In conclusion, monopronucleated ICSI zygotes present differences in developmental capacity and morphokinetic behaviour compared with 2PN ICSI zygotes, showing particular morphokinetic parameters related to pronucleus formation. Only the 1PN ICSI-derived embryos that reached the blastocyst stage have similar morphokinetic development to blastocysts from 2PN zygotes.

Keywords

ICSI1PN zygotesMonopronucleated zygotesMorphokineticsTime-lapse
Type
Research Article
Information
Zygote , Volume 28 , Issue 3 , June 2020 , pp. 217 - 222
Copyright
© Cambridge University Press 2020

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aguilar, J, Motato, Y, Escribá, MJ, Ojeda, M, Muñoz, E and Meseguer, M (2014) The human first cell cycle: Impact on implantation. Reprod Biomed Online 28, 475–84.CrossRefGoogle ScholarPubMed
Araki, E, Itoi, F, Honnma, H, Asano, Y, Oguri, H and Nishikawa, K (2018) Correlation between the pronucleus size and the potential for human single pronucleus zygotes to develop into blastocysts. J Assist Reprod Genet 35, 817–23.CrossRefGoogle ScholarPubMed
ASEBIR (2015) Cuadernos de embriología clínica. Criterios ASEBIR de Valoración Morfológica de Oocitos, Embriones Tempranos y Blastocistos Humanos. 3a Edición.Google Scholar
Athayde Wirka, K, Chen, AA, Conaghan, J, Ivani, K, Gvakharia, M, Behr, B, Suraj, V, Tan, L and Shen, S (2014) Atypical embryo phenotypes identified by time-lapse microscopy: High prevalence and association with embryo development. Fertil Steril 101, 1637–48.e5.CrossRefGoogle ScholarPubMed
Azzarello, A, Hoest, T and Mikkelsen, AL (2012) The effect of pronuclei morphology and dynamicity on live birth outcome after time-lapse culture. Hum Reprod 27, 2649–57.CrossRefGoogle Scholar
Boada, M, Gil, Y, Mateo, S, Jorda, L, Barri, PN and Veiga, A (2012) Morphokinetic differences on embryo development after normal or abnormal fertilization. American Society for Reproductive Medicine, Abstract, P-163.CrossRefGoogle Scholar
Bradley, CK, Traversa, M, Hobson, N, Gee, AJ and McArthur, SJ (2017) Clinical use of monopronucleated zygotes following blastocyst culture and preimplantation genetic screening, including verification of biparental chromosome inheritance. Reprod Biomed Online 34, 567–74.CrossRefGoogle ScholarPubMed
Campbell, A, Fishel, S, Bowman, N, Duffy, S, Sedler, M and Hickman, CFL (2013) Modelling a risk classification of aneuploidy in human embryos using non-invasive morphokinetics. Reprod Biomed Online 26, 477–85.CrossRefGoogle ScholarPubMed
Capalbo, A, Treff, N, Cimadomo, D, Tao, X, Ferrero, S, Vaiarelli, A, Colamaria, S, Maggiulli, R, Orlando, G, Scarica, C, Scott, R, Ubaldi, FM and Rienzi, L (2017) Abnormally fertilized oocytes can result in healthy live births: improved genetic technologies for preimplantation genetic testing can be used to rescue viable embryos in in vitro fertilization cycles. Fertil Steril 108, 1007–15.e3.CrossRefGoogle ScholarPubMed
Carrasco, B, Arroyo, G, Gil, Y, Gómez, MJ, Rodríguez, I, Barri, PN and Veiga, A (2017) Selecting embryos with the highest implantation potential using data mining and decision tree based on classical embryo morphology and morphokinetics. J Assist Reprod Genet 34, 983–90.CrossRefGoogle ScholarPubMed
Chatzimeletiou, K, Morrison, EE, Prapas, N, Prapas, Y and Handyside, AH (2005) Spindle abnormalities in normally developing and arrested human preimplantation embryos in vitro identified by confocal laser scanning microscopy. Hum Reprod 20, 672–82.CrossRefGoogle ScholarPubMed
Ciray, HN, Campbell, A, Agerholm, IE, Aguilar, J, Chamayou, S, Esbert, M and Sayed, S (2014) Proposed guidelines on the nomenclature and annotation of dynamic human embryo monitoring by a time-lapse user group. Hum Reprod 29, 2650–60.CrossRefGoogle ScholarPubMed
Coticchio, G, Mignini Renzini, M, Novara, P V, Lain, M, Ponti, E De, Turchi, D, Fadini, R and Dal Canto, M (2018) Focused time-lapse analysis reveals novel aspects of human fertilization and suggests new parameters of embryo viability. Hum Reprod 33, 23–31.CrossRefGoogle ScholarPubMed
Cruz, M, Garrido, N, Herrero, J, Pérez-Cano, I, Muñoz, M and Meseguer, M (2012) Timing of cell division in human cleavage-stage embryos is linked with blastocyst formation and quality. Reprod Biomed Online 25, 371–81.CrossRefGoogle ScholarPubMed
Destouni, A, Dimitriadou, E, Masset, H, Debrock, S, Melotte, C, Van Den, Bogaert K, Zamani Esteki, M, Ding, J, Voet, T, Denayer, E, de Ravel, T, Legius, E, Meuleman, C, Peeraer, K and Vermeesch, JR (2018) Genome-wide haplotyping embryos developing from 0PN and 1PN zygotes increases transferrable embryos in PGT-M. Hum Reprod 33, 2302–11.Google ScholarPubMed
Fabozzi, G, Rega, E, Starita, MF, Amendola, MG, Colicchia, A, Giannini, P and Piscitelli, C (2019) The influence of clinical and laboratory factors on the formation of monopronucleated zygotes after intracytoplasmic sperm injection (ICSI). Zygote 27, 64–8.CrossRefGoogle Scholar
Grau, N, Escrich, L, Galiana, Y, Meseguer, M, García-Herrero, S, Remohí, J and Escribá, M-J (2015) Morphokinetics as a predictor of self-correction to diploidy in tripronucleated intracytoplasmic sperm injection; derived human embryos. Fertil Steril 104, 728–35.CrossRefGoogle ScholarPubMed
Hashimoto, S, Kato, N, Saeki, K and Morimoto, Y (2012) Selection of high-potential embryos by culture in poly(dimethylsiloxane) microwells and time-lapse imaging. Fertil Steril 97, 332–7.CrossRefGoogle ScholarPubMed
Igashira, C, Otsuki, J, Furuhashi, K, Katada, Y, Sumimoto, T, Kishi, K, Matsuura, M, Mukai, M, Sumi, C, Tsuji, Y, et al. (2016) The difference in size between single pronuclei after ICSI and after IVF. American Society for Reproductive Medicine, Abstract, P. O-42.CrossRefGoogle Scholar
Itoi, F, Asano, Y, Shimizu, M, Honnma, H and Murata, Y (2015) Birth of nine normal healthy babies following transfer of blastocysts derived from human single-pronucleate zygotes. J Assist Reprod Genet 32, 1401–7.CrossRefGoogle ScholarPubMed
Iwata, K and Mio, Y (2016) Observation of human embryonic behavior in vitro by high-resolution time-lapse cinematography. Reprod Med Biol 15, 145–54.CrossRefGoogle ScholarPubMed
Joergensen, MW, Agerholm, I, Hindkjaer, J, Bolund, L, Sunde, L, Ingerslev, HJ and Kirkegaard, K (2014) Altered cleavage patterns in human tripronuclear embryos and their association to fertilization method: a time-lapse study. J Assist Reprod Genet 31, 435–42.CrossRefGoogle ScholarPubMed
Jukam, D, Shariati, SAM and Skotheim, JM (2017) Zygotic genome activation in vertebrates. Dev Cell 42, 316–32.CrossRefGoogle ScholarPubMed
Kai, Y, Iwata, K, Iba, Y and Mio, Y (2015) Diagnosis of abnormal human fertilization status based on pronuclear origin and/or centrosome number. J Assist Reprod Genet 32, 1589–95.CrossRefGoogle ScholarPubMed
Lagalla, C, Tarozzi, N, Sciajno, R, Wells, D, Santo, M Di, Nadalini, M, Distratis, V and Borini, A (2017) Embryos with morphokinetic abnormalities may develop into euploid blastocysts. Reprod Biomed Online 34, 137–46.CrossRefGoogle ScholarPubMed
Levron, J, Munné, S, Willadsen, S, Rosenwaks, Z and Cohen, J (1995) Male and female genomes associated in a single pronucleus in human zygotes. Biol Reprod 52, 653–7.CrossRefGoogle Scholar
Liao, H, Zhang, S, Cheng, D, Ouyang, Q, Lin, G, Gu, Y, Lu, C, Gong, F and Lu, G (2009) Cytogenetic analysis of human embryos and embryonic stem cells derived from monopronuclear zygotes. J Assist Reprod Genet 26, 583–9.CrossRefGoogle ScholarPubMed
Lim, AST, Goh, VHH, Su, CL and Yu, SL (2000) Microscopic assessment of pronuclear embryos is not definitive. Hum Genet 107, 62–8.CrossRefGoogle Scholar
Liu, Y, Chapple, V, Roberts, P, Ali, J and Matson, P (2014a) Time-lapse videography of human oocytes following intracytoplasmic sperm injection: Events up to the first cleavage division. Reprod Biol 14, 249–256.CrossRefGoogle ScholarPubMed
Liu, Y, Chapple, V, Roberts, P and Matson, P (2014b) Prevalence, consequence, and significance of reverse cleavage by human embryos viewed with the use of the embryoscope time-lapse video system. Fertil Steril 102, 1295–300.e2.CrossRefGoogle ScholarPubMed
Martinez, F, Rodríguez, I, Devesa, M, Buxaderas, R, Gómez, MJ and Coroleu, B (2016) Should progesterone on the human chorionic gonadotropin day still be measured? Fertil Steril 105, 86–92.CrossRefGoogle ScholarPubMed
Mateo, S, Parriego, M, Boada, M, Vidal, F, Coroleu, B and Veiga, A (2013) In vitro development and chromosome constitution of embryos derived from monopronucleated zygotes after intracytoplasmic sperm injection. Fertil Steril 99, 897–902.CrossRefGoogle ScholarPubMed
Mateo, S, Vidal, F, Parriego, M, Rodríguez, I, Montalvo, V, Veiga, A and Boada, M (2017) Could monopronucleated ICSI zygotes be considered for transfer? Analysis through time-lapse monitoring and PGS. J Assist Reprod Genet 34, 905–11.CrossRefGoogle ScholarPubMed
Meseguer, M, Herrero, J, Tejera, A, Hilligsøe, KM, Ramsing, NB and Remoh, J (2011) The use of morphokinetics as a predictor of embryo implantation. Hum Reprod 26, 2658–71.CrossRefGoogle ScholarPubMed
Milewski, R, Ku, P, Kuczyska, A, Stankiewicz, B, Ukaszuk, K and Kuczyski, W (2015) A predictive model for blastocyst formation based on morphokinetic parameters in time-lapse monitoring of embryo development. J Assist Reprod Genet 32, 571–9.CrossRefGoogle ScholarPubMed
Mio, Y, Iwata, K, Yumoto, K and Maeda, K (2014) Human embryonic behaviour observed with time-lapse cinematography. J Heal Med Informat 5, 143.Google Scholar
Montag, M, Liebenthron, J and Köster, M (2011) Which morphological scoring system is relevant in human embryo development? Placenta 32, S252–6.CrossRefGoogle ScholarPubMed
Otsu, E, Sato, A, Nagaki, M, Araki, Y and Utsunomiya, T (2004) Developmental potential and chromosomal constitution of embryos derived from larger single pronuclei of human zygotes used in in vitro fertilization. Fertil Steril 81, 723–4.CrossRefGoogle ScholarPubMed
Ottolini, CS, Kitchen, J, Xanthopoulou, L, Gordon, T, Summers, MC and Handyside, AH (2017) Tripolar mitosis and partitioning of the genome arrests human preimplantation development in vitro. Sci Rep 7, 1–10.CrossRefGoogle ScholarPubMed
Payne, D, Flaherty, SP, Barry, MF and Matthews, CD (1997) Preliminary observations on polar body extrusion and pronuclear formation in human oocytes using time-lapse video cinematography. Hum Reprod 12, 532–41.CrossRefGoogle ScholarPubMed
Rubio, I, Kuhlmann, R, Agerholm, I, Kirk, J, Herrero, J, Escribá, M-J, Bellver, J and Meseguer, M (2012) Limited implantation success of direct-cleaved human zygotes: a time-lapse study. Fertil Steril 98, 1458–63.CrossRefGoogle ScholarPubMed
Staessen, C and Van Steirteghem, AC (1997) The chromosomal constitution of embryos developing from abnormally fertilized oocytes after intracytoplasmic sperm injection and conventional in vitro fertilization. Hum Reprod 12, 321–7.CrossRefGoogle ScholarPubMed
Sultan, KM, Munné, S, Palermo, GD, Alikani, M and Cohen, J (1995) Chromosomal status of uni-pronuclear human zygotes following in vitro fertilization and intracytoplasmic sperm injection. Hum Reprod 10, 132–6.CrossRefGoogle ScholarPubMed
Tanaka, A, Yumoto, K, Iwata, K, Mizoguchi, M, Tsuneto, M and Mio, Y (2016) Dynamic analysis of human parthenogenetic zygotes induced by artificial oocyte activation. American Society for Reproductive Medicine, Abstract, P-215.CrossRefGoogle Scholar
Van Der Heijden, GW, Van Den, Berg IM, Baart, EB, Derijck, AAHA, Martini, E and Boer, P De (2009) Parental origin of chromatin in human monopronuclear zygotes revealed by asymmetric histone methylation patterns, differs between IVF and ICSI. Mol Reprod Dev 76, 101–8.CrossRefGoogle ScholarPubMed
Wong, CC, Loewke, KE, Bossert, NL, Behr, B, Jonge, CJ De, Baer, TM and Reijo Pera, RA (2010) Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol 28, 1115–1121.CrossRefGoogle ScholarPubMed
Xie, P-Y, Tang, Y, Hu, L, Ouyang, Q, Gu, Y-F, Gong, F, Leng, L-Z, Zhang, S-P, Xiong, B, Lu, G-X and Lin, G (2018) Identification of biparental and diploid blastocysts from monopronuclear zygotes with the use of a single-nucleotide polymorphism array. Fertil Steril 110, 545–54.e5.CrossRefGoogle ScholarPubMed
Yamaguchi, K, Takayanagi, T, Minamoto, H, Suzuki, N and Ando, H (2013) Time-lapse analysis of human embryos developing from mononuclear (1PN) oocytes after conventional in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). Fertil Steril 100, S385.CrossRefGoogle Scholar
Supplementary material: File

Mateo et al. supplementary material

Tables S1-S2

Download Mateo et al. supplementary material(File)
File 25.3 KB