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Optimization of sperm freezability in Bactrian camel using various dilution rates and equilibration times

Published online by Cambridge University Press:  30 September 2019

Vahid Vahedi Open the ORCID record for Vahid Vahedi [Opens in a new window] ,
Mohsen Mostafaei ,
Hossein Vaseghi Dodaran  and
Nemat Hedayat Evrigh
Vahid Vahedi*
Affiliation:
Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran
Mohsen Mostafaei
Affiliation:
Research Centre for Agriculture and Natural Resources, Ardabil 56491-11169, Iran
Hossein Vaseghi Dodaran
Affiliation:
Department of Animal Science, College of Agriculture, University of Tabriz, Tabriz, Iran
Nemat Hedayat Evrigh*
Affiliation:
Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil, Iran
*
Address for correspondence: Vahid Vahedi. Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. Tel: +98 45 32715417. Mobile: +98 914 4322355. Fax: +98 45 32715417. E-mail: vahediv@uma.ac.ir Nemat Hedayat Evrigh. Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil , Iran Fax: +98 45 33512204. E-mail: nhedayat@uma.ac.ir
Address for correspondence: Vahid Vahedi. Department of Animal Science, Moghan College of Agriculture and Natural Resources, University of Mohaghegh Ardabili, Ardabil, Iran. Tel: +98 45 32715417. Mobile: +98 914 4322355. Fax: +98 45 32715417. E-mail: vahediv@uma.ac.ir Nemat Hedayat Evrigh. Department of Animal Science, Faculty of Agricultural Sciences, University of Mohaghegh Ardabili, Ardabil , Iran Fax: +98 45 33512204. E-mail: nhedayat@uma.ac.ir
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Summary

The effect of different dilution rates and equilibration times on the cryopreservation of Bactrian camel spermatozoa was evaluated in the current study. Semen samples from four healthy adult males were collected, processed and pooled. They were then subjected to a completely randomized 4×2 factorial design including four dilution rates (DR; 1:1, 1:2, 1:4 or 1:8; v:v with SHOTOR diluent) and two equilibration times (ET; 1 or 2 h at 5ºC). After freezing and thawing, sperm kinematic parameters as well as viability, plasma membrane integrity, abnormality and seminal malondialdehyde level were assessed. According to the results, four-fold diluted samples recorded significantly higher values (P < 0.05) for sperm total (39.58 vs 31.83 and 33.33,%) and progressive motility (19.50 vs 14.00 and 14.25,%), viability (55.37 vs 43.50 and 48.75,%) and plasma membrane integrity (46.75 vs 37.25 and 37.37,%) than those of both less (1:1) and high (1:8) concentrated samples, respectively. By contrast, the percentage of abnormal spermatozoa and the concentration of seminal malondialdehyde were comparable among all treated groups. Moreover, ET revealed that 1 h equilibration had significantly higher sperm motility (37.04 vs 33.33%), linearity (42.29 vs 32.26%), beat cross-frequency (13.15 vs 8.70 Hz), plasma membrane integrity (42.25 vs 39.75%) and viability (51.37 vs 48.12%) compared with 2 h of ET (P < 0.05). Taken together, a four-fold dilution along with 1 h equilibration can be an optimal procedure to cryopreserve Bactrian camel sperm.

Keywords

Bactrian camelDilution rateEquilibration timeSemen cryopreservation
Type
Research Article
Information
Zygote , Volume 27 , Issue 6 , December 2019 , pp. 362 - 366
Copyright
© Cambridge University Press 2019 

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References

Abd El-Salaam, A (2013) Freezability of camel spermatozoa as affected by cryoprotective agents and equilibration periods added with pentoxifylline. Egypt J Basic Appl Physiol 12, 17–48.Google Scholar
Ahmad, M, Nasrullah, R and Ahmad, N (2015) Effect of cooling rate and equilibration time on pre-freeze and post-thaw survival of buck sperm. Cryobiology 70, 233–8.10.1016/j.cryobiol.2015.03.002CrossRefGoogle ScholarPubMed
Alvarez, M, Tamayo-Canul, J, Anel, E, Boixo, JC, Mata-Campuzano, M, Martinez-Pastor, F, Anel, L and de Paz, P (2012) Sperm concentration at freezing affects post-thaw quality and fertility of ram semen. Theriogenology 77, 1111–8.10.1016/j.theriogenology.2011.10.013CrossRefGoogle ScholarPubMed
Austin, A and Natarajan, N (1991) Effect of buffers and dilution rates on motility percent of chicken spermatozoa. Ind J Poult Sci 26, 34–8.Google Scholar
Blesbois, E and De Reviers, M (1992) Effect of different fractions of seminal plasma on the fertilizing ability of fowl spermatozoa stored in vitro . J Reprod Infertil 95, 263–8.10.1530/jrf.0.0950263CrossRefGoogle ScholarPubMed
Bravo, PW, Skidmore, JA and Zhao, XX (2000) Reproductive aspects and storage of semen in Camelidae. Anim Reprod Sci 62, 173–93.10.1016/S0378-4320(00)00158-5CrossRefGoogle ScholarPubMed
Chatterjee, S and Gagnon, C (2001) Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing. Mol Reprod Dev 59, 451–8.10.1002/mrd.1052CrossRefGoogle ScholarPubMed
Daskin, A, Kulaksiz, R, Ergun, A and Halil, E (2011) The effect of different dilution rates of Angora buck semen frozen with Bioxcell® extender on the post-thaw quality. J Fac Vet Med Univ Erciyes 8, 23–6.Google Scholar
Davila, MP, Munoz, PM, Bolanos, JM, Stout, TA, Gadella, BM, Tapia, JA, da Silva, CB, Ferrusola, CO and Pena, FJ (2016) Mitochondrial ATP is required for the maintenance of membrane integrity in stallion spermatozoa, whereas motility requires both glycolysis and oxidative phosphorylation. Reproduction (Camb.) 152, 683–94.CrossRefGoogle Scholar
Deen, A, Vyas, S, Jain, M and Sahani, M (2004) Refrigeratory preservation of camel semen. J Camel Pract Res 11, 137–9.Google Scholar
Elliot, F (1961) Artificial insemination of a Bactrian camel (Camelus bactrianus). Int Zoo Year Book 3, 94–5.CrossRefGoogle Scholar
Esterbauer, H and Cheeseman, KH (1990) Determination of aldehydic lipid peroxidation products: malonaldehyde and 4-hydroxynonenal. Methods Enzymol 186, 407–21.CrossRefGoogle ScholarPubMed
Fahy, GM, Lilley, TH, Linsdell, H, Douglas, MS and Meryman, HT (1990) Cryoprotectant toxicity and cryoprotectant toxicity reduction: in search of molecular mechanisms. Cryobiology 27, 247–68.10.1016/0011-2240(90)90025-YCrossRefGoogle ScholarPubMed
Fleisch, A, Malama, E, Witschi, U, Leiding, C, Siuda, M, Janett, F and Bollwein, H (2017) Effects of an extension of the equilibration period up to 96 h on the characteristics of cryopreserved bull semen. Theriogenology 89, 255–62.10.1016/j.theriogenology.2016.10.018CrossRefGoogle Scholar
Fowler, M (1997) Evolutionary history and differences between camelids and ruminants. J Camel Pract Res 4, 99–105.Google Scholar
Garner, DL, Thomas, CA, Gravance, CG, Marshall, CE, DeJarnette, JM and Allen, CH (2001) Seminal plasma addition attenuates the dilution effect in bovine sperm. Theriogenology 56, 31–40.CrossRefGoogle ScholarPubMed
Kaka, A, Wahid, H, Rosnina, Y, Yimer, N, Khumran, A, Sarsaifi, K, Behan, AA, Kaka, U and Ebrahimi, M (2015) α-Linolenic acid supplementation in Bioxcell® extender can improve the quality of post-cooling and frozen–thawed bovine sperm. Anim Reprod Sci 153, 1–7.CrossRefGoogle ScholarPubMed
Leite, TG, Do Vale Filho, VR, De Arruda, RP, De Andrade, AF, Emerick, LL, Zaffalon, FG, Martins, JA and De Andrade, VJ (2010) Effects of extender and equilibration time on post-thaw motility and membrane integrity of cryopreserved bull semen evaluated by CASA and flow cytometry. Anim Reprod Sci 120, 31–8.CrossRefGoogle ScholarPubMed
Long, JA (2006) Avian semen cryopreservation: what are the biological challenges? Poult Sci 85, 232–6.CrossRefGoogle ScholarPubMed
Malo, C, Crichton, EG and Skidmore, JA (2017) Optimization of the cryopreservation of Dromedary camel semen: cryoprotectants and their concentration and equilibration times. Cryobiology 74, 141–7.10.1016/j.cryobiol.2016.11.001CrossRefGoogle ScholarPubMed
Malo, C, Elwing, B, Soederstroem, L, Lundeheim, N, Morrell, JM and Skidmore, JA (2019) Effect of different freezing rates and thawing temperatures on cryosurvival of Dromedary camel spermatozoa. Theriogenology 125, 43–8.CrossRefGoogle ScholarPubMed
Morton, K, Gibb, Z, Bertoldo, M and Maxwell, WC (2009) Effect of diluent, dilution rate and storage temperature on longevity and functional integrity of liquid stored alpaca (Vicugna pacos) semen. J Camelid Sci 2, 15–25.Google Scholar
Morton, KM, Evans, G and Maxwell, WM (2010) Effect of glycerol concentration, Equex STM supplementation and liquid storage prior to freezing on the motility and acrosome integrity of frozen–thawed epididymal alpaca (Vicugna pacos) sperm. Theriogenology 74, 311–6.10.1016/j.theriogenology.2010.02.015CrossRefGoogle ScholarPubMed
Niasari-Naslaji, A, Mosaferi, S, Bahmani, N, Gerami, A, Gharahdaghi, AA, Abarghani, A and Ghanbari, A (2007) Semen cryopreservation in Bactrian camel (Camelus bactrianus) using SHOTOR diluent: effects of cooling rates and glycerol concentrations. Theriogenology 68, 618–25.CrossRefGoogle ScholarPubMed
Niasari-Naslaji, A, Mosaferi, S, Bahmani, N, Gharahdaghi, AA, Abarghani, A, Ghanbari, A and Gerami, A (2006) Effectiveness of a Tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen. Cryobiology 53, 12–21.10.1016/j.cryobiol.2006.03.006CrossRefGoogle ScholarPubMed
Niasari-Naslaji, A, Nikjou, D, Skidmore, JA, Moghiseh, A, Mostafaey, M, Razavi, K and Moosavi-Movahedi, AA (2009) Interspecies embryo transfer in camelids: the birth of the first Bactrian camel calves (Camelus bactrianus) from Dromedary camels (Camelus dromedarius). Reprod Fertil Dev 21, 333–7.CrossRefGoogle Scholar
Perez-Crespo, M, Moreira, P, Pintado, B and Gutierrez-Adan, A (2008) Factors from damaged sperm affect its DNA integrity and its ability to promote embryo implantation in mice. J Androl 29, 47–54.10.2164/jandrol.107.003194CrossRefGoogle ScholarPubMed
Pradiee, J, O’Brien, E, Esteso, MC, Castaño, C, Toledano-Díaz, A, López-Sebastián, A, Marcos-Beltran, JL, Vega, RS, Guillamon, FG, Martinez-Nevado, E, Guerra, R and Santiago-Moreno, J (2016) Effect of shortening the prefreezing equilibration time with glycerol on the quality of chamois (Rupicapra pyrenaica), ibex (Capra pyrenaica), mouflon (Ovis musimon) and aoudad (Ammotragus lervia) ejaculates. Anim Reprod Sci 171, 121–8.10.1016/j.anireprosci.2016.06.007CrossRefGoogle ScholarPubMed
Santiago-Moreno, J, Castaño, C, Toledano-Díaz, A, Coloma, MA, López-Sebastián, A, Prieto, MT and Campo, JL (2011) Semen cryopreservation for the creation of a Spanish poultry breeds cryobank: optimization of freezing rate and equilibration time. Poult Sci 90, 2047–53.CrossRefGoogle ScholarPubMed
Schafer, S and Holzmann, A (2000) The use of transmigration and spermac stain to evaluate epididymal cat spermatozoa. Anim Reprod Sci 59, 201–11.10.1016/S0378-4320(00)00073-7CrossRefGoogle ScholarPubMed
Seifi-Jamadi, A, Kohram, H, Shahneh, AZ, Ansari, M and Macías-García, B (2016) Quercetin ameliorate motility in frozen–thawed turkmen stallions sperm. J Equine Vet Sci 45, 73–7.10.1016/j.jevs.2016.06.078CrossRefGoogle Scholar
Shah, SA, Andrabi, SM and Qureshi, IZ (2016) Effect of equilibration times, freezing, and thawing rates on post-thaw quality of buffalo (Bubalus bubalis) bull spermatozoa. Andrology 4, 972–6.CrossRefGoogle ScholarPubMed
Viveiros, AT and Leal, MC (2016) Sperm dilution ratio affects post-thaw motility rate and velocity of Prochilodus lineatus (Characiformes) sperm. Zygote 24, 662–7.CrossRefGoogle ScholarPubMed
Zhao, XX, Huang, YM and Chen, BX (1994) Artificial insemination and pregnancy diagnosis in the Bactrian camel (Camelus bactrianus). J Arid Environ 26, 61–5.10.1006/jare.1994.1009CrossRefGoogle Scholar