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Oxidative stress induced by methomyl exposure reduces the quality of early embryo development in mice

Published online by Cambridge University Press:  10 May 2021

Daohong He Open the ORCID record for Daohong He [Opens in a new window] ,
Guobo Han ,
Xiaomeng Zhang ,
Jingyu Sun ,
Yongnan Xu ,
Qingguo Jin  and
Qingshan Gao
Daohong He
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Guobo Han
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Xiaomeng Zhang
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Jingyu Sun
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Yongnan Xu
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Qingguo Jin
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
Qingshan Gao*
Affiliation:
College of Agriculture, Yanbian University, Yanji, 133000China Engineering Research Center of North-East Cold Region Beef Cattle Science & Technology Innovation, Ministry of Education, Yanji133002, China
*
Author for correspondence: Qingshan Gao, College of Agriculture, Yanbian University, Yanji, China. E-mail: qsgao@ybu.edu.cn
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Summary

Methomyl is a widely used carbamate insecticide and environmental oestrogen that has adverse effects on the reproductive system. However, there have been no reports on the effect of methomyl on early embryos in mammals. In this study, we explored the effect of methomyl exposure on the quality of early embryonic development in mice and the possible mechanisms. During in vitro culture, different concentrations of methomyl (10, 20, 30 and 35 μM) were added to mouse zygote medium. The results showed that methomyl had an adverse effect on early embryonic development. Compared with the control group, the addition of 30 μM methomyl significantly reduced the rate of early embryo blastocyst formation. Methomyl exposure can increase oxidative stress and impair mitochondrial function, which may be the cause of blastocyst formation. In addition, we found that methomyl exposure promoted apoptosis and autophagy in mouse blastocysts. The toxic effect of methomyl on early embryos may be the result of oxidative stress induction. Taken together, our results indicate that methomyl can cause embryonic development defects in mice, thereby reducing the quality of early embryo development.

Keywords

Embryo developmentMethomylMitochondriaMouseOxidative stress
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 57 - 64
Copyright
© The Author(s), (2021). Published by Cambridge University Press

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