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Penetration of concanavalin-A-treated Chinese hamster oocytes by golden hamster spermatozoa in vitro, and chromosome analysis of hybrid 1-cell zygotes

Published online by Cambridge University Press:  26 September 2008

Seiji Watanabe ,
Hiroyuki Tateno  and
Yujiroh Kamiguchi
Seiji Watanabe*
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
Hiroyuki Tateno
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
Yujiroh Kamiguchi
Affiliation:
Department of Biological Sciences, Asahikawa Medical College, Asahikawa, Japan
*
Seiji Watanabe, Department of Biological Sciences, Asahikawa Medical College, 4–5 Nishikagura, Asahikawa 078, Japan. Telephone: +81-166-68-2731,. Fax: +81-166-65-5803
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Summary

Pretreatment of zona-free Chinese hamster (CH) oocytes with three kinds of lectin – concanavalin A (Con-A), phytohaemagglutinin-P (PHA) and wheat germ agglutinin (WGA) – was attempted in order to improve penetration by golden hamster (GH) spermatozoa in vitro. Con-A had no significant effect on penetration at 2 μg/ml, adequately facilitated oocyte-sperm fusion at 4 μg/ml, and caused excessive sperm binding and resultant severe polyspermy at 10 μg/ml. Neither PHA nor WGA had positive effects on sperm penetration at any concentrations (2–10 μg/ml) examined. Using the Con-A (4 μg/ml)pretreatment, high rates of interspecific fertilisation and subsequent chromosome analysis of hybrid 1-cell zygotes were achieved. Among 258 CH oocytes used, 212 (82.2%) were fertilised and 153 (72.2% of fertilised ova) developed to the first cleavage metaphase. Eventually, 132 CH-derived chromosome complements and 153 GH-derived ones were successfully karyoanalysed. Incidences of aneuploidy and structural anomaly were 3.1% and 2.3% in CH complements, and 1.4% and 6.5% in GH complements, respectively. These incidences were not significantly different from those obtained by intraspecific in vivo fertilisation, suggesting that our interspecific in vitro fertilisation system does not cause chromosome aberrations.

Keywords

Chinese hamster oocytesChromosomesGolden hamster spermatozoaInterspecific in vitro fertilisationLectin
Type
Article
Information
Zygote , Volume 4 , Issue 3 , August 1996 , pp. 167 - 172
Copyright
Copyright © Cambridge University Press 1996

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