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Plasminogen activatior inhibitor type 1 in rat spermatozoa: localisation in the tail, in the acrosome and on the surface of the head

Published online by Cambridge University Press:  26 September 2008

Marianne Manske ,
Beate Welte ,
Karen Hitschke ,
Andreas Wack  and
Ernesto G. Bade
Marianne Manske*
Affiliation:
Arbeitsgruppe Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Konstanz,Germany.
Beate Welte
Affiliation:
Arbeitsgruppe Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Konstanz,Germany.
Karen Hitschke
Affiliation:
Arbeitsgruppe Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Konstanz,Germany.
Andreas Wack
Affiliation:
Arbeitsgruppe Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Konstanz,Germany.
Ernesto G. Bade
Affiliation:
Arbeitsgruppe Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Konstanz,Germany.
*
M. Manske, AG Zell-und Tumorbiologie, Fakultät für Biologie, Universität Konstanz, Postfach 5560600, D-78434 Konstanz, Germany. Tel:07531-883638. Fax: 07531-882966.
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Summary

Isolated seminiferous tubules of rat testis contain considerable urokinase-inhibiting activity. An immunohistological analysis revealed the presence of plasminogen activator inhibitor type 1 (PAI-1) in the basement membrane as well as in the interior of the tubules. Distribution and intensity of the intratubular immunoreactivity depends on the tubules. Distribution and intensity of the intratubular immunoreactivity depends on the stage of the seminiferous cycle. A relatively weak signal is present around elongated nuclei of spermatids at the beginning of chromatin condensation. The signal intensity increases in the course of differentiation until a maximum is reached at stages VII-VIII. In these stages PAI-1 immunoreactivity is localised around the nuclei of the late spermatids as well as along their tails. Spermatozoa in the ductus epididymis also strongly react with the PAI-1-specific antiserum, suggesting that the inhibitor remains associated with the germ cells after spermiation and during maturation in the epididymis. In intact mature spermatozoa isolated from epididymis cauda by ‘swimming-up’ in non-capacitation medium, PAI-1 antigen is localised on the plasma membrane surrounding the head. In addition, in fixed and permeabilised cells the immunoreactivity is detectable in the acrosome and in the tail. Possible functions of PAI-1 in spermatogenesis, sperm motility and sperm-egg interaction are discussed.

Keywords

MotilityPlasminogen activator inhibitor type 1SpermatogenesisSpermatozoonSperm-egg interaction
Type
Article
Information
Zygote , Volume 2 , Issue 3 , August 1994 , pp. 243 - 252
Copyright
Copyright © Cambridge University Press 1994

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