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Sperm DNA fragmentation measured by sperm chromatin dispersion impacts morphokinetic parameters, fertilization rate and blastocyst quality in ICSI treatments

Published online by Cambridge University Press:  26 May 2021

Shikai Wang Open the ORCID record for Shikai Wang [Opens in a new window] ,
Weihong Tan ,
Yueyue Huang ,
Xianbao Mao ,
Zhengda Li ,
Xiaohui Zhang ,
Pingpin Wei  and
Lintao Xue
Shikai Wang
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Weihong Tan
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Yueyue Huang
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Xianbao Mao
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Zhengda Li
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Xiaohui Zhang
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Pingpin Wei
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
Lintao Xue*
Affiliation:
Centre of Reproductive Medicine and Genetic, the People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China
*
Author for correspondence: Lintao Xue. Department of Reproductive Medicine and Genetics Centre, People’s Hospital of Guangxi Zhuang Autonomous Region, Nanning, Guangxi, China, Taoyuan Road 6 Nanning530021, China. Tel: +86 771 2186483. E-mail: ltxgxh@163.com
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Summary

To determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

Keywords

Cleavage patternEmbryo qualityICSIMorphokinetic parametersSperm DNA fragmentation
Type
Research Article
Information
Zygote , Volume 30 , Issue 1 , February 2022 , pp. 72 - 79
Copyright
© The Author(s), 2021. Published by Cambridge University Press

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