Hostname: page-component-848d4c4894-v5vhk Total loading time: 0 Render date: 2024-06-29T19:25:41.716Z Has data issue: false hasContentIssue false
  • English
  • Français

The developmental potential of mouse somatic cell nuclear-transferred oocytes treated with trichostatin A and 5-aza-2′-deoxycytidine

Published online by Cambridge University Press:  01 May 2009

Yuta Tsuji ,
Yoko Kato  and
Yukio Tsunoda
Yuta Tsuji
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
Yoko Kato
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara, Japan.
Yukio Tsunoda*
Affiliation:
Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara 631-8505, Japan.
*
All correspondence to: Yukio Tsunoda. Laboratory of Animal Reproduction, College of Agriculture, Kinki University, Nara 631-8505, Japan. e-mail: tsunoda@nara.kindai.ac.jp
Get access Rights & Permissions [Opens in a new window]

Summary

To facilitate nuclear reprogramming, somatic cells or somatic cell nuclear-transferred (SCNT) oocytes have been treated with the histone deacetylase inhibitor trichostatin A (TSA), or the DNA methyltransferase inhibitor, 5-aza-2′-deoxycytidine (5-aza-dC), to relax epigenetic marks of differentiated somatic cells. TSA-treated SCNT oocytes have increased developmental potential, but the optimal treatment period is unknown. Reduced methylation levels in somatic cells have no positive effect on SCNT oocytes, but the treatment of SCNT embryos with 5-aza-dC has not been investigated. We examined the effect of TSA treatment duration on the developmental potential of mouse SCNT oocytes and the effect of 5-aza-dC treatment on their in vitro and in vivo developmental potential. To determine the effects of TSA treatment duration, nuclear-transferred (NT) oocytes were cultured for 0 to 26 h with 100 nM TSA. SCNT oocytes treated with TSA for 8 to 12 h had the higher rate of development to blastocysts and full-term fetuses were obtained after treatment for 8 to 12 h. When oocytes were treated for 14 h and 26 h, blastocyst rates were significantly decreased and fetuses were not obtained. To examine the effect of 5-aza-dC, 2-cell stage SCNT embryos were cultured with 10 or 100 nM 5-aza-dC for 48 h to the morula stage and transferred. The potential of embryos treated with 5-aza-dC to develop into blastocysts was decreased and no fetuses were obtained after transfer. The findings demonstrated that long-term TSA treatment of SCNT mouse oocytes and treatment with 5-aza-dC inhibit the potential to develop into blastocysts and to fetuses after transfer.

Keywords

5-Aza-dCNuclear transferSomatic cellsTSA
Type
Research Article
Information
Zygote , Volume 17 , Issue 2 , May 2009 , pp. 109 - 115
Copyright
Copyright © Cambridge University Press 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Cervoni, N. & Szyf, M. (2001). Demethylase activity is directed by histone acetylation. J. Biol. Chem. 276, 40778–87.CrossRefGoogle ScholarPubMed
Enright, B.P, Kubota, C., Yang, X. & Tian, X.C. (2003). Epigenetic characteristics and development of embryos cloned from donor cells treated by trichostatin A or 5-aza-2′-deoxycytidine. Biol. Reprod. 69, 896–901.CrossRefGoogle ScholarPubMed
Enright, B.P., Sung, L.Y., Chang, C.C., Yang, X. & Tian, X.C. (2005). Methylation and acetylation characteristics of cloned bovine embryos from donor cells treated with 5-aza-2′-deoxycytidine. Biol. Reprod. 72, 944–8.Google Scholar
Erbach, G.T., Lawitts, J.A., Papaioannou, V.E. & Biggers, J.D. (1994). Differential growth of the mouse preimplantation embryo in chemically defined media. Biol. Reprod. 50, 1027–33.CrossRefGoogle ScholarPubMed
Flach, G., Johson, M.H., Brade, P.R., Taylor, R.A. & Bolton, V.N. (1982). The transition from maternal to embryonic control in the 2-cell mouse embryo. EMBO J. 1, 681–6.Google Scholar
Fulton, B.P. & Whittingham, D.G. (1978). Activation of mammalian oocytes by intracellular injection of calcium. Nature 273, 149–51.CrossRefGoogle ScholarPubMed
Geiman, T.M. & Robertson, K.D. (2002). Chromatin remodeling, histone modifications and DNA methylation-how does it all fit together? J. Cell Biochem. 87, 117–25.Google Scholar
Jones, K.L., Hill, J., Shin, T.Y., Lui, L. & Westhusin, M. (2001). DNA hypomethylation of karyoplasts for bovine nuclear transfer. Mol. Reprod. Dev. 60, 208–13.CrossRefGoogle Scholar
Kim, J.M., Liu, S.H., Tazaki, M., Nagata, M. & Aoki, F. (2003). Change in histone acetylation during mouse oocyte meiosis. J. Cell Biol. 162, 37–46.Google Scholar
Kishigami, S., Mizutani, E., Ohta, H., Hikichi, T., Thuan, N.V., Wakayama, S. & Wakayama, T. (2006a). Significant improvement of mouse cloning technique by treatment with trichostatin A after somatic nuclear transfer. Biochem. Biophys. Res. Commun. 340, 183–9.Google Scholar
Kishigami, S., Thuan, N.V., Hikichi, T., Ohta, H., Wakayama, S., Mizutani, E., Wakayama, T. (2006b). Epigenetic abnormalities of the mouse paternal zygotic genome associated with microinsemination of round spermatids. Dev. Biol. 289, 195–205.Google Scholar
Kumar, B.M., Jin, H.F., Kim, J.G., Song, H.J., Hong, Y., Balasubramanian, S., Choe, S.Y. & Rho, G.J. (2006). DNA methylation levels in porcine fetal fibroblasts induced by an inhibitor of methylation, 5-azacytidine. Cell Tissue Res. 325, 445–54.CrossRefGoogle Scholar
Li, X.P., Kato, Y. & Tsunoda, Y. (2005). Comparative analysis of development-related gene expression in mouse preimplantation embryos with different developmental potential. Mol. Reprod. Dev. 72, 152–60.Google Scholar
Li, X.P., Kato, Y. & Tsunoda, Y. (2006). Comparative studies on the mRNA expression of development-related genes in an individual mouse blastocysts with different developmental potential. Cloning Stem Cells 8, 214–24.CrossRefGoogle Scholar
Li, X.P., Kato, Y., Tsuji, Y. & Tsunoda, Y. (2008). The effect of trichostatin A on mRNA expression of chromatin structure-, DNA methylation- and development-related genes in cloned mouse blastocysts. Cloning Stem Cells 10, 133–42.Google Scholar
Oswald, J., Engemann, S., Lane, N., Mayer, W., Olek, A., Hundele, R., Dean, W., Reik, W. & Walter, J. (2000). Active demethylation of the paternal genome in the mouse zygote. Curr. Biol. 10, 475–8.CrossRefGoogle ScholarPubMed
Rybouchkin, A., Kato, Y. & Tsunoda, Y. (2006). Role of histone acetylation in reprogramming of somatic nuclei following nuclear transfer. Biol. Reprod. 74, 1083–9.CrossRefGoogle ScholarPubMed
Shi, W., Zakhartchenko, V. & Wolf, E. (2003). Epigenetic reprogramming in mammalian nuclear transfer. Differentiation 71, 91–113.Google Scholar
Takahashi, K. & Yamanaka, S. (2006). Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663–76.Google Scholar
Tsunoda, Y. & Kato, Y. (1995). Development of enucleated mouse oocytes receiving PDGF- and FGF-treated fetal male germ cells after activation with electrical stimulation. J. Reprod. Dev. 41, 71–5.CrossRefGoogle Scholar
Tsunoda, Y. & McLaren, A. (1983). Effect of various procedures on the viability of mouse embryos containing half the normal number of blastomeres. J. Reprod. Fertil. 69, 315–22.Google Scholar
Vignon, X., Zhou, Q. & Renard, J.P. (2002). Chromatin as a regulative architecture of the early developmental functions of mammalian embryos after fertilization or nuclear transfer. Cloning Stem Cells 4, 363–77.Google Scholar
Wade, P.A. & Kikyo, N. (2002). Chromatin remodeling in nuclear cloning. Eur. J. Biochem. 269, 2284–7.Google Scholar
Wakayama, T., Perry, A.C., Zuccotti, M., Johnson, K.R. & Yanagimachi, R. (1998). Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei. Nature 394, 369–74.Google Scholar
Wee, G., Shim, J.J., Koo, D.B., Chae, J.I., Lee, K.K. & Han, Y.M. (2007). Epigenetic alteration of the donor cells does not recapitulate the reprogramming of DNA methylation in cloned embryos. Reproduction 134, 781–7.Google Scholar
Yabuuchi, A., Yasuda, Y., Kato, Y. & Tsunoda, Y. (2004). Effects of nuclear transfer procedures on ES cell cloning efficiency in the mouse. J. Reprod. Dev. 50, 263–8.Google Scholar
Yang, X., Smith, S.L, Tian, X.C., Lewin, H.A., Renard, J.P. & Wakayama, T. (2007). Nuclear reprogramming of cloned embryos and its implications for therapeutic cloning. Nat. Genet. 39, 295–300.Google Scholar
Zhang, Y., Li, J., Villemoes, K., Pedersen, A.M., Purup, S. & Vajta, G. (2007). An epigenetic modifier results in improved in vitro blastocyst production after somatic cell nuclear transfer. Cloning Stem Cells 9, 357–63.CrossRefGoogle ScholarPubMed