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The effect of temperature fluctuations on the cytoskeletal organisation and chromosomal constitution of the human oocyte

Published online by Cambridge University Press:  26 September 2008

Paula A. Almeida  and
Virginia N. Bolton
Paula A. Almeida
Affiliation:
Assisted Conception unit, King's College School of Medicine and Dentistry, London, UK.
Virginia N. Bolton*
Affiliation:
Assisted Conception unit, King's College School of Medicine and Dentistry, London, UK.
*
V.N. Bolton, Assisted Conception Unit, Department of Obstetrics and Gynaecology, King's College School of Medicine and Dentistry, Denmark Hill, London SE5 8RX, UK. Telephone: + 44 (0171)-346 3158. Fax: + 44 (0171)-274 3242. e-mail: RCJB190@BAY.CC.KCL.AC.UK.
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Summary

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The effect of temperature fluctuation on spindle integrity and chromosomal organisation in the human oocyte, and the consequences of such effects on the chromosomal constitution of resulting parthenotes, were investigated. A total of 340 oocytes were stained immunocytochemically with an antibody to α-tubulin, and 502 were activated parthenogenetically. Exposure of oocytes to room temperature for 2, 10 or 30 min caused disruption of the spindle in 77%(n = 26),72%(n = 18) and 89% (n = 19) of cases respectively, with evidence of chromosomal dispersal in 50%,56% and 52.6% respectively. These effects were reversed when oocytes were returned to 37°C after exposure to room temperature for 2 min, but not after 10 min or 30 min: Temperature reduction affected rates of parthenogenetic activation of oocytes (2 min: 67%, n = 27; 10 min: 68%, n = 28; 30 min; 54%, n = 35) and cleavege of resulting parthenotes, but only if oocytes were exposed to room temperature for 30 min (30 min: 53%, n = 19).There is a direct association between temperature-induced spindle damage in the oocyte(70%, 50 of 63) and chromosomal abnormalities in parthenotes developed from oocytes exposed to room temperature (56%,23 of 41; P < 0.01).

Keywords

Chromosomal abnormalitiesCytoskeletonHumanIVFOocyteSpindleTemperature changes
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 357 - 365
Copyright
Copyright © Cambridge University Press 1995

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