Hostname: page-component-848d4c4894-p2v8j Total loading time: 0 Render date: 2024-06-08T11:19:03.890Z Has data issue: false hasContentIssue false
  • English
  • Français

The effect of temperature fluctuations on the cytoskeletal organisation and chromosomal constitution of the human oocyte

Published online by Cambridge University Press:  26 September 2008

Paula A. Almeida  and
Virginia N. Bolton
Paula A. Almeida
Affiliation:
Assisted Conception unit, King's College School of Medicine and Dentistry, London, UK.
Virginia N. Bolton*
Affiliation:
Assisted Conception unit, King's College School of Medicine and Dentistry, London, UK.
*
V.N. Bolton, Assisted Conception Unit, Department of Obstetrics and Gynaecology, King's College School of Medicine and Dentistry, Denmark Hill, London SE5 8RX, UK. Telephone: + 44 (0171)-346 3158. Fax: + 44 (0171)-274 3242. e-mail: RCJB190@BAY.CC.KCL.AC.UK.
Rights & Permissions [Opens in a new window]

Summary

Core share and HTML view are not available for this content. However, as you have access to this content, a full PDF is available via the ‘Save PDF’ action button.

The effect of temperature fluctuation on spindle integrity and chromosomal organisation in the human oocyte, and the consequences of such effects on the chromosomal constitution of resulting parthenotes, were investigated. A total of 340 oocytes were stained immunocytochemically with an antibody to α-tubulin, and 502 were activated parthenogenetically. Exposure of oocytes to room temperature for 2, 10 or 30 min caused disruption of the spindle in 77%(n = 26),72%(n = 18) and 89% (n = 19) of cases respectively, with evidence of chromosomal dispersal in 50%,56% and 52.6% respectively. These effects were reversed when oocytes were returned to 37°C after exposure to room temperature for 2 min, but not after 10 min or 30 min: Temperature reduction affected rates of parthenogenetic activation of oocytes (2 min: 67%, n = 27; 10 min: 68%, n = 28; 30 min; 54%, n = 35) and cleavege of resulting parthenotes, but only if oocytes were exposed to room temperature for 30 min (30 min: 53%, n = 19).There is a direct association between temperature-induced spindle damage in the oocyte(70%, 50 of 63) and chromosomal abnormalities in parthenotes developed from oocytes exposed to room temperature (56%,23 of 41; P < 0.01).

Keywords

Chromosomal abnormalitiesCytoskeletonHumanIVFOocyteSpindleTemperature changes
Type
Article
Information
Zygote , Volume 3 , Issue 4 , November 1995 , pp. 357 - 365
Copyright
Copyright © Cambridge University Press 1995

References

Almeida, P.A. & Bolton, V.N. (1993). Immaturity and chromosomal abnormalities in oocytes that fail to develop pronuclei following insemination in vitro. Hum. Reprod. 8, 229–32.CrossRefGoogle ScholarPubMed
Bolton, V.N., Hawes, S.M., Taylor, C.T. & Parsons, J.H. (1989). Development of spare human preimplantation embryos in vitro: an analysis of the correlations among gross morphology, cleavage rates, and development to the blastocyst. J. IVF and ET 6, 30–5.Google Scholar
Dustin, P. (1984). Microtubules, 2ndedn. Berlin: Springer.CrossRefGoogle Scholar
Eichenlaub-Ritter, U., Stahl, A. & Luciani, J.M. (1988). The microtubular cytoskeleton and chromosomes of unfertilised human oocytes aged in vitro. Hum. Genet. 80, 259–64.CrossRefGoogle Scholar
Gifford, D.J., Fleetham, J.A., Mahadevan, M.M., Taylor, P.J. & Schultz, G.A. (1987). Protein synthesis in mature human oocytes. Gamete Res. 18, 97–107.CrossRefGoogle ScholarPubMed
Kola, I., Kirby, C.Shaw, J., Davey, A. & Trounson, A. (1988). Vitrification of mouse oocytes results in aneuploid zygotes and malformed fetuses. Teratology 38, 467–74.CrossRefGoogle ScholarPubMed
Ma, S., Kalousek, D.K., Zouves, C., Yuen, B.H., Gomel, V. & Moon, Y.S. (1990). The chromosomal complements of cleaved human embryos resulting from in vitro fertilisation. J. IVF and ET. 7, 16–21.Google Scholar
Maro, B., Howlett, S.K., & Webb, M. (1985). Non-spindle microtubule organizing centres in metaphase II-arrested mouse oocytes. J. Cell Biol. 101, 1665–72.CrossRefGoogle ScholarPubMed
Ortiz, M.E., Salvatierra, A.M. & Lopel, J., Fernandez, E. & Croxatto, H.B. (1982),Post-ovulatory ageing of human ova.I.Light microscopic observations. Gamete Res. 6, 11–17.CrossRefGoogle Scholar
Pellestor, F., Girardet, A., Andreo, B., Arnal, F. & Humeau, C. (1994). Relationship between morphology and chromosomal constitution in human preimplantation embryos. Mol Reprod.Dev. 39, 141–6.CrossRefGoogle Scholar
Pickering, S.J., Johnson, M.H. (1987). The influence of cooling on the organisation of the meiotic spindle of the mouse oocyte. Hum. Reprod. 2, 207–16.CrossRefGoogle ScholarPubMed
Pickering, S.J., Johnson, M.H., Braude, P.R. & Houliston, E. (1988). Cytoskeletal organisation in fresh, aged and spontaneously activated human oocytes. Hum Reprod. 3, 978–98.CrossRefGoogle ScholarPubMed
Pickering, S.J., Cant, A., Braude, P.R., Currie, J. & Johnson, M.H. (1990). Transient cooling to room temperature can cause irreversible disruption of the meiotic spindle in the human oocyte. Feril. Steril. 54, 102–8.CrossRefGoogle ScholarPubMed
Plachot, M., Veiga, A., Montagut, J., de Grouchy, J., Calderon, G., Lepretre, S., Junca, A.M., Santalo, J., Carles, E., Mandelbaum, J., Barri, P., Degoy, J., Cohen, J., Egozcue, J., Sabatier, J.C. & Salat-Baroux, J. (1988). Are clinical and biological IVF parameters correlated with chromosomal disorders in early life: a multicentric study. Hum. Reprod. 3, 627–35.CrossRefGoogle ScholarPubMed
Sathananthan, A.H., Trounson, A., Freeman, L. & Brady, T. (1988). The effects of cooling human oocytes. Hum. Reprod. 3, 968–77.CrossRefGoogle ScholarPubMed
Sathananthan, A.H., Kirby, C., Peura, A. & Trounson, A. (1992). Mouse oocyte cooling. J. Assist. Reprod. Genet. 9, 139–48.CrossRefGoogle ScholarPubMed
Tarín, J.J., Gómez, E. & Pellicer, A. (1991). Chromosome anomalies in human oocytes in vitro. Fertil. Steril. 55, 964–9.CrossRefGoogle ScholarPubMed
Waterstone, J.J. & Parsons, J.H. (1992). A prospective study to investigate the value of flushing follicles during transvaginal ultrasound-directed follicle aspiration. Fertil Steril. 57, 221–3.CrossRefGoogle ScholarPubMed