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Expression and localization of Nlrp4g in mouse preimplantation embryo

Published online by Cambridge University Press:  11 November 2014

Hui Peng ,
Xiujiao Lin ,
Wenhao Li  and
Wenchang Zhang
Hui Peng
Affiliation:
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China.
Xiujiao Lin
Affiliation:
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China.
Wenhao Li
Affiliation:
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China.
Wenchang Zhang*
Affiliation:
College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China.
*
All correspondence to: Wenchang Zhang. College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou, Fujian, 350002, People's Republic of China. Tel: +86 591 83758852. Fax: +86 591 83758565. e-mail: zwcfz1221@126.com
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Summary

The Nlrp gene family contains 20 members and plays a pivotal role in the innate immune and reproductive systems in the mouse. During evolution, seven Nlrp4 gene copies (named from Nlrp4a to Nlrp4g). Nlrp4a–Nlrp4g have arisen that display specific or preferential ovarian expression patterns. However, the expression pattern and localization of Nlrp4g in mouse preimplantation embryo development are unknown. Here we report that Nlrp4g was highly expressed in mature oocytes and zygotes, then downregulated and not detected after the 2-cell embryo stage. NLRP4G protein remained present through the blastocyst stage and was mainly localized in the cytoplasm. Furthermore, overexpression of Nlrp4g in zygotes did not affect normal development in terms of the rate of blastocyst formation. These results provide the first evidence that NLRP4G is a maternal factor that may play essential role during zygotic genome activation in the mouse.

Keywords

ExpressionLocalizationMouseNlrp4gPreimplantation embryo
Type
Research Article
Information
Zygote , Volume 23 , Issue 6 , December 2015 , pp. 846 - 851
Copyright
Copyright © Cambridge University Press 2014 

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