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Morphological evaluation of adult domestic cat testicular biopsy after vitrification

Published online by Cambridge University Press:  13 May 2024

Julyne Vivian Guimarães de Carvalho Open the ORCID record for Julyne Vivian Guimarães de Carvalho [Opens in a new window] ,
Airton Renan Bastos Soares Open the ORCID record for Airton Renan Bastos Soares [Opens in a new window] ,
Inara Tayná Alves Evangelista Open the ORCID record for Inara Tayná Alves Evangelista [Opens in a new window] ,
Danuza Leite Leão Open the ORCID record for Danuza Leite Leão [Opens in a new window] ,
Regiane Rodrigues dos Santos Open the ORCID record for Regiane Rodrigues dos Santos [Opens in a new window]  and
Sheyla Farhayldes Souza Domingues Open the ORCID record for Sheyla Farhayldes Souza Domingues [Opens in a new window]
Julyne Vivian Guimarães de Carvalho
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Programme in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil
Airton Renan Bastos Soares
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Inara Tayná Alves Evangelista
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Danuza Leite Leão
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Regiane Rodrigues dos Santos
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil
Sheyla Farhayldes Souza Domingues*
Affiliation:
Laboratory of Wild Animal Biotechnology and Medicine, Faculty of Veterinary Medicine, Federal University of Pará, Castanhal, Pará, Brazil Postgraduate Programme in Animal Health and Production in the Amazon, Federal Rural University of Amazonia, Belém, Pará, Brazil
*
Corresponding author: Sheyla Farhayldes Souza Domingues; Email: shfarha@ufpa.br
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Summary

Testicular biopsies (9 mm3) from domestic cats (n = 10) submitted to orchiectomy were submitted to equilibrium vitrification in the presence of ethylene glycol (EG) alone or combined with dimethylsulfoxide (DMSO) as intracellular cryoprotectants, and sucrose or trehalose as extracellular cryoprotectants. The samples were vitrified with 40% EG or 20% EG + 20% DMSO, plus 0.1 M or 0.5 M of sucrose or trehalose. The study was divided into Step 1 and Step 2. In Step 1, intratubular cells (spermatogonia, spermatids, spermatocytes, and Sertoli cells) were quantified and classified as intact or degenerated (pyknotic and/or vacuolated cells). Cryodamage of seminiferous cords was determined by spermatogonia and Sertoli cell scoring of nuclei alterations, tubular basement membrane detachment, epithelium shrinkage, and tubular measures (total area, epithelium area, larger and smaller diameter, and height of the epithelium). In Step 2, Hoechst 33342 stain and propidium iodide (PI) fluorescent stain were used to assess the cell viability of the four best experimental groups in Step 1. The effect of treatments on all analyses was accessed using analysis of variance (ANOVA), and Fisher’s post hoc test at P < 0.05 significance was considered. In Step 1, the mean percentage of spermatogonia and Sertoli cells morphological integrity did not show a difference when using both sugars at different concentrations, but their morphology was more affected when DMSO was used. EG use associated with 0.1 M of sucrose or trehalose positively affected spermatocyte and spermatid morphology, respectively. The larger diameter and epithelium height of seminiferous tubules were increased using DMSO plus 0.5 M sucrose and DMSO plus 0.1 M trehalose. The changes in spermatogonial/Sertoli nucleoli visualization were best scored in the EG groups, while the nuclei condensation was lower with sucrose. The basement membrane was satisfactorily preserved with 0.1 M sucrose. In Step 2, the percentage of cell viability was higher when EG plus 0.1 M sucrose was used. Therefore, DMSO’s negative effect on the vitrification of testicular biopsies of adult domestic cats was evident. The EG plus 0.1 M of sucrose or trehalose associations are the most suitable CPAs to preserve the testicular histology structure of adult domestic cats in vitrification.

Keywords

CryopreservationCryoprotectantsDomestic catTesticular tissueVitrification
Type
Research Article
Information
Zygote , First View , pp. 1 - 8
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Copyright
© The Author(s), 2024. Published by Cambridge University Press

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