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Structural and functional differentiation of follicular and oviductal mouse oocytes visualised with FITC-protein conjugates

Published online by Cambridge University Press:  26 September 2008

Haekwon Kim  and
Allen W. Schuetz
Haekwon Kim
Affiliation:
The Johns Hopkins University, School of Hygiene and Public Health, Departement of population Dynamics, Baltimore, Maryland, USA.
Allen W. Schuetz*
Affiliation:
The Johns Hopkins University, School of Hygiene and Public Health, Departement of population Dynamics, Baltimore, Maryland, USA.
*
Allen W. Schuetz, PhD, The Johns Hopkins University, Department of Population Dyamics, 615 N. Wolfe Street, Baltimore, MD 21205, USA. Teoephone: 301-955-3117. Fax: 301-955-0792.
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Summary

The fluroscence labelling characteristics of mouse oocytes were examined at various stages of periovulatory differentiation using FITC-protein conjugates. The zona pellucida perivitelline space and plasma membrane underwent visible changes which were developmentally and environmentally related. Following exposure to fluorescein isothiocyanate (FITC)-casein conjugates, the zona pellucida (ZP) of germinal vesicle stage (GV) ovarian oocytes exhibited a bright, amorphous, mesh-like staining pattern (immature type). In contrast, mature polar body stage (PB) oocytes, either ovarian or oviductal, displayed faint, spotty fluorescence labelling of the ZP (mature type). The perivitelline space (PVS) of mature ovarian oocytes (12 h post-hCG) failed to label, whereas approximately 50% of oviductal oocytes showed PVS labelling. The incidence of PVS staining increased with postovulatory age, possibly as a result of the accumulation of materials secreted by the oviduct. Following in vivo or in vitro fertilisation of oocytes, a characteristic pattern of plasma membrane (PM) labelling was observed. Similar patterns of PM labelling were seen in oocytes parthenogenetically activated with ethanol or ionophore (A23187) but not in control oocytes. The pattern of PM labelling observed with FITC-protein conjugates was strikingly similar to that observed with FITC-labelled lectins, which are thought to interact with glycoconjugates released from cortical granules. Immature type of ZP staining also occurred when GV oocytes were treated with FITC alone or with a variety of FITC-protein conjugates. Thus, protein may not be required for labelling of the ZP by FITC-protein conjugates as previously thought. FITCconjugated proteins including casein, bovine serum albumin, peroxidase and non-immune immunoglobulin G (IgG), all labelled the PM of activated oocytes; however, FITC-IgG failed to label the PVS. Results demonstrate for the first time that various components of viable mouse oocytes exhibit and undergo characteristic structural and functional changes during periovulatory differentiation as evidenced by their interaction with one or more FITC-protein conjugates and/or FITC. On the basis of these results the intrafollicular and oviductal mechanisms mediating these changes are discussed as is the possibility that the fluorescent molecule attached to conjugates may play a role in oocyte labelling.

Keywords

Oocyte maturationPervitelline spacePlasma membraneZona pellucida
Type
Article
Information
Zygote , Volume 1 , Issue 4 , November 1993 , pp. 297 - 307
Copyright
Copyright © Cambridge University Press 1993

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References

Alexandre, H., Van Cauwenberge, A. & Mulnard, J. (1989). Involvement of mimicrotubules and microfilaments in the control of the nuclear movement during maturation of mouse oocytes. Dev. Biol.. 136, 311–320.CrossRefGoogle Scholar
Bleil, J.D. & Wassarman, P.M. (1980). Structure and function of the zona pellucida: identification and characterisation of the proteins of the mouse oocyte's zona pellucida. Dev. Biol. 76, 185–202.CrossRefGoogle ScholarPubMed
Canipari, R., Bevilacqua, A., Colonna, R., De Felici, M. & Mangia, F. (1988). Actin synthesis is not regulated by granulosa cells in mouse growing and preovulatory oocytes. Gamete Res. 20, 115–124.CrossRefGoogle Scholar
Cherr, G.N., Drobins, E.A. & Katz, D.F. (1988). Localisation of cortical granule constituents before and after exocytosis in the hamster egg. J. Exp. Zool. 246, 81–93.CrossRefGoogle ScholarPubMed
Cuthbertson, K.S.R. (1983). Parthenogenetic activation of mouse oocytes in vitro. J. Exp. Zool. 226, 311–314.CrossRefGoogle ScholarPubMed
De Felici, M., Dolci, S. & Siracusa, G. (1989). Influence of cumulus cell processes on oolemma permeability and lethality of isolated mouse oocytes cultured in Ca2+-free medium. Gamete Res. 23, 245–253.CrossRefGoogle ScholarPubMed
Ducibella, T., Anderson, E., Albertini, D.F., Aalberg, J. & Rangarajan, S. (1988). Quantitative studies of changes in cortical granule number and distribution in the mouse oocyte during meiotic maturation. Dev. Biol. 130, 184–197.CrossRefGoogle ScholarPubMed
Ducibella, T., Kurasawa, S., Rangarajan, S., Kopf, G.S. & Schultz, R.M. (1990). Precocious loss of cortcial granules during mouse oocytes meiotic maturation and correlation with an egg-induced modification of the zona pellucida. Dev. Biol. 137, 46–55.CrossRefGoogle ScholarPubMed
East, I.J. & Dean, J. (1984). Monoclonal antibodies as probes of the distribution of ZP-2, the major sulfated glycoprotein of the murine zona pellucida. J. Cell. Biol. 98, 795–800.CrossRefGoogle ScholarPubMed
Eppig, J.J. (1979). Gonadotropin stimulation of the expansion of cumulus oophori isolated from mice: general conditions for expansioin in vitro. J. Exp. Zool. 208, 111–120.CrossRefGoogle ScholarPubMed
Fowler, R.E. & Grainge, C. (1985). A histochemical study of the changes occurring in the protein-carbohydrate composition of the cumulus-oocyte complex and zona pellucida in immature mice in response to gonadotropin stimulation. Histochem. J. 17, 1235–1249.CrossRefGoogle Scholar
Gilula, N.B., Epstein, M.L. & Beers, W.H. (1978). Cell-to-cell communication. a study of the cumulus-oocyte complex. J. Cell Biol. 78, 58–75.CrossRefGoogle ScholarPubMed
Greve, J.M., Salzmann, G.S., Roller, R.J. & Wassarman, P.M. (1982). Biosynthesis of the major Zona pellucida glycoprotein secreted by oocytes during mammalian oogenesis. Cell 31, 749–759.CrossRefGoogle ScholarPubMed
Gulyas, B.J. & Schmell, E.D. (1980). Ovoperoxidase activity in ionophore treated mouse eggs. I. Electron microscopic localisation. Gamete Res. 3, 267–278.CrossRefGoogle Scholar
Gulyas, B.J. & Yuan, L.C. (1985). Cortical reaction and zona hardening in mouse oocytes following exposure to ethanol. J. Exp. Zool. 233, 269–276CrossRefGoogle Scholar
Kapur, R.P. & Johnson, L.V. (1985). An oviductal fluid glycoprotrein associated with ovulated mouse ova and early embryos. Dev. Biol. 112, 89–93.CrossRefGoogle ScholarPubMed
Kapur, R.P. & Johnson, L.V. (1986). Selective sequestration of an oviductal fluid glycorotein in the perivitelline space of mouse oocytes and embryos. J. Exp. Zool. 238, 249–60.CrossRefGoogle Scholar
Kapur, R.P. & Johnson, L.V. (1988). Ultrastructural evidence that specialized regions of the murine oviduct contribute a glycoprotein to the extracellular matrix of mouse oocytes. Anat. Rec. 221, 720–729.CrossRefGoogle Scholar
Kaufman, M.H., Fowler, R.E., Barratt, E. & McDougall, R.D. (1989). Ultrastructural and histochemical changes in the murine zona pellucida during the final stages of oocyte maturation prior to ovulation. Gamete Res. 24, 35–48.CrossRefGoogle ScholarPubMed
Kim, H. & Schuetz, A.W. (1991). Regulation of parthenogenetic activation of metaphase II mouse oocytes in vitro by pyruvate. J. Exp. Zool. 251, 375–385.CrossRefGoogle Scholar
Lee, S.H., Ahuja, K.K., Gilburt, D.J. & Whittingham, D.J. (1988). The appearance of glycoconjugates associated with cortical granule release during mouse fertilisation. Development 102, 595–604.CrossRefGoogle Scholar
Maro, B., Johnson, M.H.Webb, M. & Flach, G. (1986). Mechansism of polar body formation in the mouse oocyte:an interaction between the chromosomes, the cytoskeleton and the plasma membrane. J. Embryol. Exp. Morphol. 92, 11–32.Google Scholar
Nicholson, G.L., Yanagimachi, R. & Yanagimachi, H. (1975). Ultrastructural localization of lectin-binding sites on the zona-pellucidae and plasma membranes of mammalian eggs. J. Cell Biol. 66, 263–274.CrossRefGoogle Scholar
Nicosia, S.V., Wolf, D.P. & Inoue, M. (1977). Cortical granule distribution and cell surface characteristics in mouse eggs. Dev. Biol. 57, 56–4.CrossRefGoogle ScholarPubMed
Schatten, G., Simerly, C. & Schatten, H. (1985). Microtubule configuration during fertilisation, mitosis and early development in the mouse. Proc. Natl. Acad. Sci. USA 82, 4152–4156.CrossRefGoogle ScholarPubMed
Schuetz, A.W. 1985. Local control mechanisms during oogenesis and folliculogenesis. In: ‘Developmental Biology’ ed. Browder, L.W., Vol. 1, pp. 3–83. New York: Plenum Press.Google Scholar
Van Blerkom, J. & Bell, H. (1986). Regulation of development in the fully grown mouse oocye: chromosomemediated temporal and spatial differentiation of the cytoplasm and plasma membrane. J. Embryol. Exp. Morphol. 93, 213–238.Google Scholar
Wassarman, P.M. 1988, Zona pellucida glycoproteins. Annu. Rev. Biochem. 57, 414–442.CrossRefGoogle ScholarPubMed
Yanagimachi, R. (1988). Mammalian fertilisation. In: The physiology of Reproduction, ed. Knobil, E. & Neill, J.D., vol. 1, pp. 135–185. New York: Raven Press.Google Scholar