Hostname: page-component-cd9895bd7-hc48f Total loading time: 0 Render date: 2024-12-30T14:31:58.540Z Has data issue: false hasContentIssue false
  • English
  • Français

Expression of dendritic cell phenotypic antigens in cervical lymph nodes of patients with hypopharyngeal and laryngeal carcinoma

Published online by Cambridge University Press:  22 May 2009

X Li ,
Y Takahashi ,
K Sakamoto  and
T Nakashima
X Li
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shanghai Children's Hospital, Shanghai Jiaotong University, China Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
Y Takahashi
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
K Sakamoto
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
T Nakashima*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume, Japan
*
Address for correspondence: Dr Tadashi Nakashima, Department of Otolaryngology-Head and Neck Surgery, Kurume University School of Medicine, Kurume 830-0011, Japan. Fax: +81 942 37 1200 E-mail: orlkaku@med.kurume-u.ac.jp
Get access Rights & Permissions [Opens in a new window]

Abstract

Background:

The purpose of this study was to assess the presence of dendritic cell phenotypic antigens in the cervical lymph nodes of patients with hypopharyngeal and laryngeal carcinoma, and to assess the significance of such antigens in the tumour immune reaction.

Methods:

Immunohistochemical staining of cervical lymph nodes was performed using antibodies against cell surface markers such as S-100 protein and cluster of differentiation 1a and 83 glycoproteins. Two hundred and seventy-four cervical lymph nodes obtained at surgery from 37 patients with hypopharyngeal carcinoma and 31 patients with laryngeal carcinoma were thus evaluated.

Results:

The number of dendritic cells positive for each phenotypic antigen was significantly greater in non-metastatic lymph nodes than in metastatic lymph nodes. In the metastatic lymph nodes, cluster of differentiation 1a glycoprotein positive dendritic cells were predominantly detected in the cancer ‘nest’, whereas mature dendritic cells staining for cluster of differentiation 83 glycoprotein were prominent in the peritumour area. In the metastatic lymph nodes, in contrast to the cluster of differentiation 1a glycoprotein positive dendritic cells, the degree of infiltration of cluster of differentiation 83 glycoprotein positive dendritic cells was significantly higher in the peritumour area than in the cancer nest. There was a significant difference in survival status, comparing patients with different degrees of dendritic cell infiltration for each type of phenotypic antigen.

Conclusions:

Dendritic cells may play different roles in tumour immunity against hypopharyngeal and laryngeal carcinoma. The phenotypic antigens of dendritic cells may thus constitute important indices with which to predict the prognosis of patients with hypopharyngeal and laryngeal carcinoma.

Keywords

Dendritic CellCD1aCD83S-100Lymph Node
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 123 , Supplement S31 , May 2009 , pp. 5 - 10
Copyright
Copyright © JLO (1984) Limited 2009

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1Banchereau, J, Steinman, RM. Dendritic cells and the control of immunity. Nature 1998;392:245–52CrossRefGoogle ScholarPubMed
2Caux, C, Massacrier, C, Vanbervliet, B, Dubois, B, de Saint-Vis, B, ezutter-Dambuyant, C, Jacquet, C, Schmitt, D, Banchereau, J. CD34+ hematopoietic progenitors from human cord blood differentiate along two independent dendritic cell pathways in response to GM-CSF + TNF alpha. Adv Exp Med Biol 1997;417:21–5CrossRefGoogle ScholarPubMed
3Chang, CC, Wright, A, Punnonen, J. Monocyte-derived CD1a+ and CD1a- dendritic cell subsets differ in their cytokine production profiles, susceptibilities to transfection and capacities to direct Th cell differentiation. J Immunol 2000;165:3584–91CrossRefGoogle ScholarPubMed
4Pawele, CG, Zeuthen, J, Kiessling, R. Escape from host-antitumor immunity. Crit Rev Oncog 1997;8:111–41CrossRefGoogle Scholar
5Sogn, JA. Tumor immunology: the glass is half full. Immunity 1998;9:757–63CrossRefGoogle ScholarPubMed
6Schuler, G, Steinman, RM. Dendritic cells as adjuvant for immune mediated resistance to tumor. J Exp Med 1997;186:1183–8CrossRefGoogle Scholar
7Girolomoni, G, Ricciardi-Castagnoli, P. Dendritic cells hold promise for immunotherapy. Immunol Today 1997;18:102–4CrossRefGoogle ScholarPubMed
8Steinman, RM. The dendritic cell system and its role in immunogenicity. Ann Rev Immunol 1991;9:271–96CrossRefGoogle ScholarPubMed
9Menetrier-Caux, C, Montmain, G, Dieu, MC et al. Inhibition of the differentiation of dendritic cells from CD34(+) progenitor by tumor cells: role of interleukin-6 and macrophage colony-stimulating factor. Blood 1998;92:4778–91CrossRefGoogle ScholarPubMed
10Pawelec, G, Zeuthen, J, Kiessling, R. Escape from host-antitumor immunity. Crit Rev Oncog 1997;8:111–14CrossRefGoogle ScholarPubMed
11Wolenski, M, Cramer, SO, Ehrlich, S, Steeg, C, Grossschupff, G, Tenner-Racz, K, Racz, P, Fleischer, B, von Bonin, A. Expression of CD83 in the murine immune system. Med Microbiol Immunol (Berl) 2003;4:189–92CrossRefGoogle Scholar
12Xia, CQ, Kao, KJ. Monocyte-derived CD1a+ dendritic cells generated in two different culture systems: immunophenotypic and functional comparison. Scand J Immunol 2003;57:324–32CrossRefGoogle ScholarPubMed
13Peh, WC, Shek, TW, Ng, IO, Lo, CM, Fan, S, Ngan, H. Imaging of follicular dendritic cell tumours of the liver. J Gastroenterol Hepatol 1998;13:1146–51CrossRefGoogle ScholarPubMed
14Tsujitani, S, Kakeji, Y, Watanabe, A et al. Infiltration of dendritic cells in relation to tumor invasion and lymph node metastasis in human gastric cancer. Cancer 1990;66:2012–163.0.CO;2-N>CrossRefGoogle ScholarPubMed
15Maluccio, MA, Rao, J, Sharma, V, Lagman, M, Suthanthiran, M. Dendritic cells armed with anti-CD3 mAbs reduce pulmonary metastases, prolong survival, and engender anti-tumor effector cells demonstrable by adoptive transfer. Ann Surg Oncol 2000;7:771–6CrossRefGoogle Scholar
16Sprinzl, GM, Hussl, B, Obrist, P, Yoneda, K, Thumfaft, WF, Romani, N, Schrott-Fischer, A. Dendritic cells in precancerous lesions of the larynx. Laryngoscope 2000;110:13–18CrossRefGoogle ScholarPubMed
17Dong, P, Li, X, Zhu, Z, Yu, Z, Lu, G, Sun, Z, Wang, S. Application of tissue microarray: evaluation of the expression of S-100-positive dendritic cells, tumor suppressor gene p63 and tissue inhibitor of metalloproteinase-1 in laryngeal carcinoma. Acta Otolaryngol 2004;124:1204–7CrossRefGoogle ScholarPubMed
18Karakök, M, Bayazit, YA, Ucak, R, Ozer, E, Kanlikama, M, Mumbuc, S, Sari, I. Langerhans cell related inflammatory reaction in laryngeal squamous cell carcinoma. Auris Nasus Larynx 2003;30:81–4CrossRefGoogle ScholarPubMed
19Yilmaz, T, Gedikoglu, G, Celik, A, Onerci, M, Turan, E. Prognostic significance of Langerhans cell infiltration in cancer of the larynx. Otolaryngol Head Neck Surg 2005;132:309–16CrossRefGoogle ScholarPubMed
20Nancy, JP, Aysegul, S, Kelly, KH, Elizabeth, AG. Analysis of dendritic cells in tumor-free and tumor-containing sentinel lymph nodes from patients with breast cancer. Breast Cancer Res 2004;6:408–15Google Scholar