Hostname: page-component-cd9895bd7-p9bg8 Total loading time: 0 Render date: 2024-12-21T10:54:16.644Z Has data issue: false hasContentIssue false
  • English
  • Français

Involvement of focal adhesion kinase in cellular proliferation, apoptosis and prognosis of laryngeal squamous cell carcinoma

Published online by Cambridge University Press:  11 September 2012

D W Li ,
Y J Sun ,
Z F Sun  and
P Dong
D W Li
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, China
Y J Sun
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, China
Z F Sun
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, China
P Dong*
Affiliation:
Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai, China
*
Address for correspondence: Dr P Dong, Department of Otolaryngology-Head and Neck Surgery, Shanghai Jiaotong University Affiliated First People's Hospital, Shanghai 200080, China Fax: 86 21 63240825 E-mail: dongpin_lidawei@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Objective:

This study aimed to investigate the relationship between focal adhesion kinase expression and clinicopathological factors, cell apoptosis and proliferation, and overall survival, in laryngeal squamous cell carcinoma.

Methods:

Immunohistochemical staining was used to detect the expression of focal adhesion kinase and proliferating cell nuclear antigen in tissue samples. Apoptotic cells were assessed using the terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate nick end labelling (‘TUNEL’) method.

Results:

The proportion of focal adhesion kinase expression was 73.26 per cent in tumour tissues, significantly greater than in normal tissues (p < 0.05), and was significantly related to laryngeal cancer clinical stage, lymph node metastasis and overall survival (p < 0.05). In the tumour cases assessed, up-regulation of focal adhesion kinase expression was significantly associated with decreased cell apoptosis and increased proliferation (p < 0.05).

Conclusion:

These findings suggest that focal adhesion kinase may affect laryngeal squamous cell carcinoma progression through regulation of cell apoptosis and proliferation. Focal adhesion kinase expression is a major prognostic factor in laryngeal cancer patients.

Keywords

Laryngeal NeoplasmsCarcinoma, Squamous CellFocal Adhesion KinaseApoptosisCell ProliferationPrognosis
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1127 - 1133
Copyright
Copyright © JLO (1984) Limited 2012

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

1 Chu, EA, Kim, YJ. Laryngeal cancer: diagnosis and preoperative work-up. Otolaryngol Clin North Am 2008;41:673–95CrossRefGoogle ScholarPubMed
2 Tímár, J, Csuka, O, Remenár, E, Répássy, G, Kásler, M. Progression of head and neck squamous cell cancer. Cancer Metastasis Rev 2005;24:107–27CrossRefGoogle ScholarPubMed
3 Golubovskaya, VM. Focal adhesion kinase as a cancer therapy target. Anticancer Agents Med Chem 2010;10:735–41CrossRefGoogle ScholarPubMed
4 Wang, GJ, Ma, YP, Yang, Y, Zhang, N, Wang, W, Liu, SY et al. RNA interference-mediated silencing of focal adhesion kinase inhibits growth of human malignant glioma xenograft in nude mice. Cell Biol Int 2011;35:841–8CrossRefGoogle ScholarPubMed
5 Lei, K, Ye, L, Yang, Y, Wang, GJ, Jiang, QY, Jiang, Y et al. RNA interference-mediated silencing of focal adhesion kinase inhibits growth of human colon carcinoma xenograft in nude mice. J Biomed Nanotechnol 2010;6:272–8CrossRefGoogle ScholarPubMed
6 Huang, AL, Wan, Y, Liao, DY, Hu, HZ, Wei, L, Wang, XH et al. Suppression of human MDA-MB-435S tumor by U6 promoter-driven short hairpin RNAs targeting focal adhesion kinase. J Cancer Res Clin Oncol 2010;136:1229–42CrossRefGoogle ScholarPubMed
7 Xia, J, Lv, N, Hong, Y, Li, C, Tao, X, Chen, X et al. Increased expression of focal adhesion kinase correlates with cellular proliferation and apoptosis during 4-nitroquinoline-1-oxide-induced rat tongue carcinogenesis. J Oral Pathol Med 2009;38:524–9CrossRefGoogle ScholarPubMed
8 Beierle, EA, Ma, X, Trujillo, A, Kurenova, EV, Cance, WG, Golubovskaya, VM. Inhibition of focal adhesion kinase and src increases detachment and apoptosis in human neuroblastoma cell lines. Mol Carcinog 2010;49:224–34CrossRefGoogle ScholarPubMed
9 Park, JH, Lee, BL, Yoon, J, Kim, J, Kim, MA, Yang, HK et al. Focal adhesion kinase (FAK) gene amplification and its clinical implications in gastric cancer. Hum Pathol 2010;41:1664–73CrossRefGoogle ScholarPubMed
10 Cai, L, Han, J, Zhuo, X, Xiong, Y, Dong, J, Li, X. Overexpression and significance of focal adhesion kinase in hepatocellular carcinoma and its relationship with HBV infection. Med Oncol 2009;26:409–14CrossRefGoogle ScholarPubMed
11 Hayashi, A, Aishima, S, Inoue, T, Nakata, K, Morimatsu, K, Nagai, E et al. Decreased expression of focal adhesion kinase is associated with a poor prognosis in extrahepatic bile duct carcinoma. Hum Pathol 2010;41:859–66CrossRefGoogle ScholarPubMed
12 Canel, M, Secades, P, Rodrigo, JP, Cabanillas, R, Herrero, A, Suarez, C et al. Overexpression of focal adhesion kinase in head and neck squamous cell carcinoma is independent of FAK gene copy number. Clin Cancer Res 2006;12:3272–9CrossRefGoogle ScholarPubMed
13 Rodrigo, JP, Cabanillas, R, Secades, P, Canel, M, Chiara, MD, Suárez, C. Relationship between FAK and P53 expression in squamous cell carcinomas of the larynx [in Spanish]. Acta Otorrinolaringol Esp 2007;58:347–51Google ScholarPubMed
14 Rodrigo, JP, Dominguez, F, Suárez, V, Canel, M, Secades, P, Chiara, MD. Focal adhesion kinase and E-cadherin as markers for nodal metastasis in laryngeal cancer. Arch Otolaryngol Head Neck Surg 2007;133:145–50CrossRefGoogle ScholarPubMed
15 Hussein, MR, Cullen, K. Molecular biomarkers in HNSCC: prognostic and therapeutic implications. Expert Rev Anticancer Ther 2001;1:116–24CrossRefGoogle ScholarPubMed
16 Li, JJ, Yang, XM, Wang, SH, Tang, QL. Prognostic role of epidermal growth factor-like domain 7 protein expression in laryngeal squamous cell carcinoma. J Laryngol Otol 2011;125:1152–7CrossRefGoogle ScholarPubMed
17 Marioni, G, Blandamura, S, Lionello, M, Giacomelli, L, Staffieri, C, Fasanaro, E et al. Nuclear MASPIN expression relates to a better prognosis in elderly patients with laryngeal carcinoma. Acta Otolaryngol 2011;131:1220–5CrossRefGoogle ScholarPubMed
18 Chen, H, Zhou, L, Wan, G, Dou, T, Tian, J. BMI1 promotes the progression of laryngeal squamous cell carcinoma. Oral Oncol 2011;47:472–81CrossRefGoogle ScholarPubMed
19 Kamo, N, Naomoto, Y, Shirakawa, Y, Yamatsuji, T, Hirota, S, Fujiwara, Y et al. Involvement of focal adhesion kinase in the progression and prognosis of gastrointestinal stromal tumors. Hum Pathol 2009;40:1643–9CrossRefGoogle ScholarPubMed
20 Alenzi, FQ. Links between apoptosis, proliferation and the cell cycle. Br J Biomed Sci 2004;61:99–102 CrossRefGoogle ScholarPubMed
21 Hipfner, DR, Cohen, SM. Connecting proliferation and apoptosis in development and disease. Nat Rev Mol Cell Biol 2004;5:805–15CrossRefGoogle ScholarPubMed