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Morphological assessment of the luminal surface of olfactory epithelium in mice deficient in tissue plasminogen activator following bulbectomy

Published online by Cambridge University Press:  19 September 2012

N Makino ,
S Ookawara ,
S Madoiwa ,
Y Ohta ,
T Ishikawa ,
K Katoh ,
S Takigami ,
T Kanazawa ,
O Matsuo  and
M Ichikawa
...Show all authors
N Makino*
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Ookawara
Affiliation:
Department of Anatomy, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Madoiwa
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
Y Ohta
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
T Ishikawa
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
K Katoh
Affiliation:
Department of Anatomy, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
S Takigami
Affiliation:
Laboratory of Anatomy and Cellular Biology, Department of Medical Technology, Faculty of Health Sciences, Kyorin University, Hatioji, Tokyo, Japan
T Kanazawa
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
O Matsuo
Affiliation:
Department of Physiology IIy, Kinki University School of Medicine, Osakasayama City, Japan
M Ichikawa
Affiliation:
Laboratory of Anatomy and Cell Biology, Department of Neuroscience Basic Technology, Tokyo Metropolitan Institute for Neuroscienceo, Fuchu, Toky, Japan
J Mimuro
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
Y Sakata
Affiliation:
Division of Cell and Molecular Medicine, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
K Ichimura
Affiliation:
Department of Otolaryngology–Head and Neck Surgery, Center for Molecular Medicine, Jichi Medical University School of Medicine, Shimotsuke, Tochigi, Japan
*
Address for correspondence: Dr Nobuko Makino, Department of Otolaryngology – Head and Neck Surgery, Jichi Medical University School of Medicine, 3311-1 Yakushiji, Shimotsuke, Tochigi 329-0498, Japan Fax: +81 285 44 5547 E-mail: n-makino@jichi.ac.jp
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Abstract

Objective:

This study aimed to investigate the function of tissue plasminogen activator in the olfactory epithelium of mice following neural injury.

Method:

Transmission electron microscopy was used to study the changes in the morphology of the olfactory epithelium 1–7 days after surgical ablation of the olfactory bulb (bulbectomy).

Results:

Prior to bulbectomy, a uniformly fine material was observed within some regions of the olfactory epithelium of mice deficient in tissue plasminogen activator. At 2–3 days after bulbectomy, there were degenerative changes in the olfactory epithelium. At 5–7 days after bulbectomy, we noted drastic differences in olfactory epithelium morphology between mice deficient in tissue plasminogen activator and wild-type mice (comparisons were made using findings from a previous study). The microvilli seemed to be normal and olfactory vesicles and receptor neuron dendrites were largely intact in the olfactory epithelium of mice deficient in tissue plasminogen activator.

Conclusion:

The tissue plasminogen activator plasmin system may inhibit the regeneration of the olfactory epithelium in the early stages following neural injury.

Keywords

MouseMicroscopyElectronTransmissionPlasminogen ActivatorsOlfactionPathophysiologyRegeneration
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 126 , Issue 11 , November 2012 , pp. 1114 - 1120
Copyright
Copyright © JLO (1984) Limited 2012

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