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Bacillus thuringiensis crystal protein toxicity against plant-parasitic nematodes

Published online by Cambridge University Press:  27 June 2008

Yu Zi-Quan ,
Wang Qian-Lan ,
Liu Bin ,
Zou Xue ,
Yu Zi-Niu  and
Sun Ming
Yu Zi-Quan
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Wang Qian-Lan
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Liu Bin
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Zou Xue
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Yu Zi-Niu
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
Sun Ming*
Affiliation:
State Key Laboratory of Agricultural Microbiology, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070, China
*
*Corresponding author. E-mail: m98sun@mail.hzau.edu.cn
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Abstract

A bioassay method was developed to use the parasporal crystal protein of Bacillus thuringiensis against plant-parasitic nematodes. Using this method, the parasporal crystal proteins of ten Bt strains showed activity against plant-parasitic nematodes. The toxicity of YBT-021 against Meloidogyne hapla, Pratylenchus scribneri, Tylenchorhynchus sp., Ditylenchus destructor and Aphelenchoides sp. was also assayed. The resulting LC50 values were 35.62 μg/ml, 75.65 μg/ml, 94.31 μg/ml, 215.21 μg/ml and 128.76 μg/ml, respectively.

Keywords

Bacillus thuringiensisparasporal crystal proteinbioassayplant-parasitic nematodes
Type
Research Papers
Information
Chinese Journal of Agricultural Biotechnology , Volume 5 , Issue 1 , April 2008 , pp. 13 - 17
Copyright
Copyright © China Agricultural University 2008

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Footnotes

First published in Journal of Agricultural Biotechnology 2007, 15(5): 867–871

References

Bone, LW, Bottjer, KP and Gill, SS (1985) Trichostrongylus colubriformis: egg lethality due to Bacillus thuringiensis crystal toxin. Experimental Parasitology 60(3): 314–322.CrossRefGoogle ScholarPubMed
Bone, LW, Bottjer, KP and Gill, SS (1986) Trichostrongylus colubriformis: isolation and characterization of ovicidal activity from Bacillus thuringiensis israelensis. Experimental Parasitology 62(2): 247–253.CrossRefGoogle ScholarPubMed
Bone, LW, Bottjer, KP and Gill, SS (1987) Alteration of Trichostrongylus colubriformis egg permeability by Bacillus thuringiensis israelensis toxin. Journal of Parasitology 73(2): 295–299.CrossRefGoogle ScholarPubMed
Bradfish, GA, Hickle, LA, Flores, R and Schwab, G (1991) Nematocidal Bacillus thuringiensis toxins: Opportunities in animal health and plant protection. First International Symposium on Bacillus thuringiensis, Abstracts, Oxford, p. 33.Google Scholar
Bradford, MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 72(1): 248–254.CrossRefGoogle ScholarPubMed
Cappello, M, Bungiro, RD, Harrison, LM, et al. (2006) A purified Bacillus thuringiensis crystal protein with therapeutic activity against the hookworm parasite Ancylostoma ceylanicum. Proceedings of the National Academy of Sciences of the USA 103(41): 15154–15159.CrossRefGoogle ScholarPubMed
Chen, PS (2001) The main types, characteristics and action mechanisms of nematicides. Pesticide Science and Administration 22(2): 33–35.Google Scholar
Duan, YX and Wu, G (2002) Control of Plant Nematode Disease. Beijing: China Agriculture Press.Google Scholar
Ignoffo, CM and Dropin, VH (1977) Deleterious effects of thermostable toxin of Bacillus toxin of Bacillus thuringiensis on species of soil-inhabiting myceliophagous and plant-parasitic nematodes. Journal of the Kansas Entomology Society 50(3): 394–398.Google Scholar
Prasad, SSV, Tilsk, KVR and Gollakota, KG (1972) Role of Bacillus thuringiensis var. thuringiensis on the larval survivability and egg hatching of Meloidogyne spp. the causative agent of root-knot disease. Journal of Invertebrate Pathology 20(3): 377–378.CrossRefGoogle Scholar
Sambrook, J, Fritsch, EF and Maniatis, T (2002) Molecular Cloning: A Laboratory Manual, 2nd ed. New York: Cold Spring Harbor Laboratory Press.Google Scholar
Sasser, JN and Freckman, DW (1987) A world perspective on nematology. In: Veech, JA and Dickson, DW (editors) Vistas on Nematology. Hyattsville, MD: Society of Nematologists, pp. 7–14.Google Scholar
Schnepf, E, Crickmore, N, Rie, JV, et al. (1998) Bacillus thuringiensis and its pesticidal crystal proteins. Microbiology Molecular Biology Reviews 62(3): 775–806.CrossRefGoogle ScholarPubMed
Sun, M, Luo, X, Dai, J, et al. (1996) Evaluation of Bacillus sphaericus strains from Chinese soils toxic to mosquito larvae. Journal of Invertebrate Pathology 68(1): 74–77.CrossRefGoogle ScholarPubMed
Wei, JZ, Hale, K, Carta, L, et al. (2003) Bacillus thuringiensis crystal protein that target nematodes. Proceedings of the National Academy of Sciences of the USA 100(5): 2760–2765.CrossRefGoogle ScholarPubMed
Yu, ZQ, Zhou, Y, Sun, M and Yu, ZN (2004) Progress of research on activity of Bacillus thuringiensis against plant-parasitic nematodes. Acta Phytophylacica Sinica 31(4): 418–424.Google Scholar
Zou, X, Yu, ZQ, Sun, M and Yu, ZN (2004) Screening new Bacillus thuringiensis strains with activity to root-knot nematode by a staining method. Journal of Wuhan University (Natural Science Edition) 50(S2): 127–130.Google Scholar