Hostname: page-component-77c89778f8-gvh9x Total loading time: 0 Render date: 2024-07-17T06:32:12.292Z Has data issue: false hasContentIssue false
  • English
  • Français

Comparison of the proteins of middle ear effusion with human mast cell proteins

Published online by Cambridge University Press:  29 June 2007

Yoseph Rakover ,
Amir Shneyour ,
Gabriel Rosen  and
Yaacov Lensky
Yoseph Rakover
Affiliation:
Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel
Amir Shneyour
Affiliation:
Department of the Clinical Immunology Laboratory, Central Emek Hospital, Afula, Israel
Gabriel Rosen
Affiliation:
Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel
Yaacov Lensky*
Affiliation:
Hebrew University, Faculty of Agriculture, Israel.
*
Y. Rakover, Department of Otorhinolaryngology, Central Emek Hospital, Afula, Israel.
Get access Rights & Permissions [Opens in a new window]

Abstract

In order to clarify the role of mast cells in the aetiology of secretory otitis media (SOM), we compared the protein components of middle ear effusion (MEE) with human mast cells using acrylamide gradient gel electrophoresis and electrofocusing methods. This first direct comparison between the proteins of MEE and human mast cells has been made possible by a method developed in our laboratory for cultivation of human mast cells in tissue culture.

On electrophoresis, we found that out of 12 bands of MEE proteins that were different from the serum, seven (58 per cent) had a similar electrophoretic migration rate (Rx) to mast cells. On electrofocusing, three of the four bands of MEE had a similar Rx to the mast cells. We have shown that proteins of mast cells and MEE had similar Rxs. Therefore, our study supports previous studies which suggests that mast cells play an important role in the aetiology of SOM.

Keywords

Otitis media with effusionMast cells
Type
Main Articles
Information
The Journal of Laryngology & Otology , Volume 109 , Issue 12 , December 1995 , pp. 1146 - 1150
Copyright
Copyright © JLO (1984) Limited 1995

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

References

Aim, P. E., Bloom, G. D., Helistrom, S., Saien, B., Stenfors, L. E. (1982) The release of histamine from the pars flaccida mast cells — one cause of otitis media with effusion? Acta Oto-laryngologica 94: 517–522.Google Scholar
Bachert, C., Keilmann, A., Ganzer, U. (1989) Middle ear effusion and allergy in childhood. Laryngo-rhino-otologie 68: 201–203.Google Scholar
Berger, G., Hawke, M., Proops, SW., Ranadive, N. S., Wong, D. (1984) Histamine levels in middle ear effusions. Acta Oto-laryngologica 98: 385–390.Google Scholar
Bernstein, J. M. (1993) The role of IgE-mediated hypersensitivity in the development of otitis media with effusion: a review. Otolaryngology—Head and Neck Surgery 109: 611–620.Google Scholar
Bernstein, J. M., Steger, R., Bock, N. (1978) The kallikreinkinin system in otitis media with effusion. Otolaryngology—Head and Neck Surgery 86: 249–255.CrossRefGoogle ScholarPubMed
Biosvert, P., Wasserman, S. I., Schiff, M., Ryan, A. F. (1985) Histamine-induced middle ear effusion and mucosai histopathology in the guinea pig. Annals of Otology, Rhinology and Laryngology 94: 212–216.Google Scholar
Csaba, G. (1969) Mechanism of the formation of mast cells granules. Acta Biologica Academiae Scientiarum Hun garicae 20: 205–210.Google Scholar
Finotto, S., Dolovich, J., Denburg, J. A., Jordana, M., Marshall, J. S. (1994) Functional heterogeneity of mast cells isolated from different microenvironments within nasal polyp tissue. Clinical and Experimental Immunology 95: 343–350.Google Scholar
Hellstrom, S., Salen, B., Stenfors, L. E. (1982) The site of initial production and transport of effusion materials in otitis media serosa. Acta Oto-laryngologica 93: 435–440.Google Scholar
Hurst, D. S. (1990) Allergy management of refractory serous otitis media. Otolaryngology—Head and Neck Surgery 102: 664–669.CrossRefGoogle ScholarPubMed
Jung, T. T. K., Juhn, S. K. (1986) Leukotrienes and other lipoxygenase products in middle ear effusions. In Acute and Secretory Otitis Media. (Sade, J., ed.), Kugler Publications, Amsterdam/Berkeley, pp 269–273.Google Scholar
Karlsson, C., Davis, H., Dhman, J., Andersson, U. B. (1973) Analytical thin layer gel electrofocusing in polyacrylamide gel. Applied Note L.K.B. 75: 1–13.Google Scholar
Kitamura, Y., Kasugai, T., Arizono, N., Matsuda, H. (1993) Development of mast cells and basophils: processes and regulation mechanisms. (Review) American Journal of the Medical Sciences 306: 185–191.Google Scholar
Lensky, Y., Rakover, Y. (1983) Separate protein body compartment of the worker honey bee (Apis mellifera L.). Comparative Biochemistry and Physiology 75B: 607–615.Google Scholar
Mogi, G., Tomonaga, K., Watanabe, T., Chen, T. (1992) The role of type I allergy in secretory otitis media and mast cells in the middle ear mucosa. Acta Oto-laryngologica (suppl) 493: 155–163.Google Scholar
Nakata, J., Suzuki, M., Kawauchi, H., Mogi, G. (1992) Experimental otitis media with effusion induced by middle ear effusion. Laryngoscope 102: 1037–1042.CrossRefGoogle ScholarPubMed
Rhee, C. K., Jung, T. T. K., Miller, S., Weeks, D. (1993) Experimental otitis media with effusion induced by platelet- activating factor. Annals of Otology, Rhinology and Laryngology 102: 600–605.Google Scholar
Salen, B., Hellstrom, S., Stenfors, L. E. (1984) Experimentally induced otitis media with effusion. Ada Oto-laryngologica (suppl) 414: 67–70.CrossRefGoogle ScholarPubMed
Widemar, L., Bloom, G. D., Hellstrom, S., Stenfors, L. E. (1984) Mast cells in the pars flaccida of the tympanic membrane. Acta Oto-laryngologica (suppl) 414: 115–117.CrossRefGoogle ScholarPubMed