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Thermodynamic analysis of ion effects on the binding and conformational equilibria of proteins and nucleic acids: the roles of ion association or release, screening, and ion effects on water activity

Published online by Cambridge University Press:  17 March 2009

M. Thomas Record Jr ,
Charles F. Anderson  and
Timothy M. Lohman
M. Thomas Record Jr
Affiliation:
Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706
Charles F. Anderson
Affiliation:
Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706
Timothy M. Lohman
Affiliation:
Department of Chemistry, University of Wisconsin, Madison, Wisconsin 53706
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Extract

The purpose of this review is to examine the various effects of low- molecular-weight electrolytes on the associations and interactions of proteins and nucleic acids. Our primary interest is in general electrostatic effects, rather than chemical effects (specific interactions) of particular ions (e.g. transition metals, protons). We consider those interactions in which a variation in salt concentration has a significant effect on the macromolecular equilibrium, and analyse the effects of salt in these situations in terms of (i) direct participation of ions in the biopolymer reaction, (ii) Debye—Hückel screening by salt ions of the charge interactions on the biopolymers, and (iii) the reduction in water activity brought about at high salt concentrations.

Type
Research Article
Information
Quarterly Reviews of Biophysics , Volume 11 , Issue 2 , May 1978 , pp. 103 - 178
Copyright
Copyright © Cambridge University Press 1978

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