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High Pressure Freezing/Freeze Substituion: Comparison of Chemical Fixation Versus Cryoimmobilization of Candida Albicans Cultured in Cellulose Tubing

Published online by Cambridge University Press:  02 July 2020

S. Erlandsen ,
A Holzer ,
M. Gavin ,
C. Frethem  and
C. Wells
S. Erlandsen
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, MN55455
A Holzer
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, MN55455
M. Gavin
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, MN55455
C. Frethem
Affiliation:
Department of Cell Biology and Neuroanatomy, University of Minnesota, Minneapolis, MN55455
C. Wells
Affiliation:
Department of Lab Medicine and Pathology, University of Minnesota, Minneapolis, MN55455
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Extract

In the interactions of Candida albicans with host cells, the cell wall of the yeast may play important roles in the adhesion of yeast cells to tissues. The outer cell wall of yeast (e.g. Saccharomyces cerevisiae, C. albicans) has been shown to consist of a dense network of radially projecting fibrils composed of mannoproteins that are known as fimbriae and which previously have required cryopreservation either by jet propane freezing or by plunge freezeing for their visualization. High pressure freezing provides an advantage over jet or plunge freezing in terms of the higher consistancey in the quality of freezing, and the minimization of formation of ice I with this method. Hohenberg et al reported a method utilizing cellulose capillary tubes to cryoimmobilize suspensions of microoganisms by high pressure freezing (HPF) and freeze substitution (FS), and herein, we describe an adaptation of this method by culturing microorganisms within the tubing to increase cell density prior to high pressure freezing and freeze substution.

Type
Cryotechniques, Immunocytochemistry, and Electron Microscopy II. Cells and Tissues
Information
Microscopy and Microanalysis , Volume 5 , Issue S2: Proceedings: Microscopy & Microanalysis '99, Microscopy Society of America 57th Annual Meeting, Microbeam Analysis Society 33rd Annual Meeting, Portland, Oregon August 1-5, 1999 , August 1999 , pp. 434 - 435
Copyright
Copyright © Microscopy Society of America

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References

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