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Increase Transforming Growth Factor-Beta and its Receptors in Human Renal Tissue with Rejection

Published online by Cambridge University Press:  02 July 2020

H. Song ,
J. Papadimitriou ,
C. Drachenberg ,
M. R. Weir  and
C. Wei
H. Song
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
J. Papadimitriou
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Drachenberg
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
M. R. Weir
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
C. Wei
Affiliation:
Cardiorenal Molecular Research, Departments of Surgery, Medicine and Pathology, University of Maryland School of Medicine, Baltimore, MD, 21201
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Extract

Transforming growth factor-bate (TGF-β) is a growth-relating peptide that has been shown to enhance collagen production in vivo and in vitro. TGF-β isoforms include TGF-β 1, β2 and β3. TGF-β receptors subtypes include type I (TGFβRI) and type II (TGFβRII) receptors. Overproduction of TGF-β can lead to real damage.

Renal graft rejection is major problem after kidney transplantation with severe renal damage. We hypothesized that renal tissue level of TGF-β may increase in renal rejection after kidney transplantation. Therefore, the current study was designed to determine the TGF-β 1 isoform and its receptor TGFβRI in human renal tissue with rejection by immunohistochemical staining (IHCS) and compared with normal human renal tissue. The results of IHCS was evaluated by IHCS staining density scores (0, no staining; 1, minimal staining; 2, mild staining; 3, moderate staining; and 4, strong staining). The positive staining area (+%) in entire section was also determined. The sections treated with preabsorbed blocking peptide or nonimmune rabbit serum demonstrated no immunoperoxidase activity.

Type
Pathology
Information
Microscopy and Microanalysis , Volume 6 , Issue S2: Proceedings: Microscopy & Microanalysis 2000, Microscopy Society of America 58th Annual Meeting, Microbeam Analysis Society 34th Annual Meeting, Microscopical Society of Canada/Societe de Microscopie de Canada 27th Annual Meeting, Philadelphia, Pennsylvania August 13-17, 2000 , August 2000 , pp. 614 - 615
Copyright
Copyright © Microscopy Society of America

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References

References:

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5. This research was supported in part by grants from the NIH (HL03174 & HL61299, C. Wei), AHAMD, NKF and University of Maryland School of Medicine.Google Scholar